After oral administration several gut-binding lectins induce accumulation of liquor and amylase in the proximal small intestine (2). Orally administered Phaseolus vulgaris phytohaemagglutinin (PHA) was used to study the mediation of these effects in rats. The regulation of amylase secretion clearly differed from that of the liquor. The amylase activity was of pancreatic origin, in agreement with the known cholecystokinin-releasing effect of PHA. It appears that CCK exerts its effect both directly and by facilitating neural stimulatory pathways. Intestinal secretion was identified as the source of the liquor, without a contribution by other secretions. It was mediated by a local cholinergic reflex with the involvement of both muscarinic and nicotinic acetylcholine receptors. It is speculated that the observed enteric reflex may enable the gut to transport secreted antibacterial peptides or secretory antibodies from the crypts to adherent bacteria on adjacent villi.
Bardocz S, Grant G, Ewen SWB, Duguid TJ, Brown DS, Englyst K, Pusztai A: Reversible effect of phytohaemagglutinin on the growth and metabolism of rat gastrointestinal tract. Gut 37, 353-360 (1995)
'Reversible effect of phytohaemagglutinin on the growth and metabolism of rat gastrointestinal tract ' () 37 Gut : 353 -360.
Bardocz S, Grant G, Pusztai A, Franklin MF, de Carvalho A: The effect of phytohaemagglutinin at different dietary concentrations on the growth, body composition and plasma insulin of the rat. Br. J. Nutr. 76, 613-626 (1996)
'The effect of phytohaemagglutinin at different dietary concentrations on the growth, body composition and plasma insulin of the rat ' () 76 Br. J. Nutr. : 613 -626.
Baintner K, Kiss P, Pfüller U, Bardocz S, Pusztai A: Effect of orally and intraperitoncally administered plant lectins on food consumption of rats. Acta Physiol. Hung. 90, 97-107 (2003)
'Effect of orally and intraperitoncally administered plant lectins on food consumption of rats ' () 90 Acta Physiol. Hung. : 97 -107.
Baintner K, Kiss P, Bardocz S, Pusztai A: Effect orally administered plant lectins on intestinal liquor accumulation and amylase activity in rats. Acta Physiol. Hung. 91, 73-81 (2004)
'Effect orally administered plant lectins on intestinal liquor accumulation and amylase activity in rats ' () 91 Acta Physiol. Hung. : 73 -81.
Furness JB, Jones C, Nurgali K, Clerc N: Intrinsic primary afferent neurons and nerve circuits within the intestine. Progress in Neurobiology, in the press (2004)
'Intrinsic primary afferent neurons and nerve circuits within the intestine ' () Progress in Neurobiology .
Green BT, Brown DR: Active bicarbonate-dependent secretion evoked by 5-hydroxy-tryptamine in porcine ileal mucosa is mediated by opioid-sensitive enteric neurons. Eur. J. Pharmacol. 451, 185-190 (2002)
'Active bicarbonate-dependent secretion evoked by 5-hydroxy-tryptamine in porcine ileal mucosa is mediated by opioid-sensitive enteric neurons ' () 451 Eur. J. Pharmacol. : 185 -190.
Herzig KH, Bardocz S, Grant G, Nustede R, Folsch UR, Pusztai A: Red kidney bean lectin is a potent cholecystokinin releasing stimulus in the rat inducing pancreatic growth. Gut 41, 333-338 (1997)
'Red kidney bean lectin is a potent cholecystokinin releasing stimulus in the rat inducing pancreatic growth ' () 41 Gut : 333 -338.
Jancsó M, Jancsó-Gábor A, Szolcsányi J: Direct evidence for neurogenic inflammation and its prevention by denervation and pretreatment with capsaicin. Br. J. Pharmacol. Chemother. 31, 138-151 (1967)
'Direct evidence for neurogenic inflammation and its prevention by denervation and pretreatment with capsaicin ' () 31 Br. J. Pharmacol. Chemother. : 138 -151.
Jordinson M and Baintner K, unpublished.
Jordinson M, Playford RJ, Calam J: Effects of a panel of dietary lectins on cholecystokinin release in rats. Am. J. Physiol. 273, G946-G950 (1997)
'Effects of a panel of dietary lectins on cholecystokinin release in rats ' () 273 Am. J. Physiol. : G946 -G950.
Kimura H, Ito S, Ohta T, Asano T, Nakazato Y: Vasoactive intestinal peptide released by acetylcholine in the dog ileum. J. Autonomic Nervous System 48, 167-174 (1994)
'Vasoactive intestinal peptide released by acetylcholine in the dog ileum ' () 48 J. Autonomic Nervous System : 167 -174.
King TP, Pusztai A, Grant G, Slater D: Immunogold localization of ingested kidney bean (Phaseolus vulgaris) lectins in epithelial cells of the rat small intestine. Histochem. J. 18, 413-420 (1986)
'Immunogold localization of ingested kidney bean (Phaseolus vulgaris) lectins in epithelial cells of the rat small intestine ' () 18 Histochem. J. : 413 -420.
Kordás K, Burghardt B, Kisfalvi K, Bardocz S, Pusztai A, Varga G: Diverse effects of phytohaemagglutinin on gastrointestinal secretions in rats. J. Physiol. (Paris) 94, 31-36 (2000)
'Diverse effects of phytohaemagglutinin on gastrointestinal secretions in rats ' () 94 J. Physiol. (Paris) : 31 -36.
Marcon-Genty D, Krempf M, Tome D: Transmucosal transport of phytohemagglutinin, concanavalin A and alpha-lactalbumin across rabbit ileum in vitro. Gastroenterol. Clin. Biol. 15, 10-15 (1991)
'Transmucosal transport of phytohemagglutinin, concanavalin A and alpha-lactalbumin across rabbit ileum in vitro ' () 15 Gastroenterol. Clin. Biol. : 10 -15.
Pusztai A, Ewen SWB, Grant G, Peumans WJ, van Damme EJM, Rubio L, Bardocz S: The relationship between survival and binding of plant lectins during small intestinal passage and their effectiveness as growth factors. Digestion 46 (Suppl. 2), 308-316 (1990)
'The relationship between survival and binding of plant lectins during small intestinal passage and their effectiveness as growth factors ' () 46 Digestion : 308 -316.
Barthó L, Stein C, Herz A: Involvement of capsaicin-sensitive neurones in hyperalgesia and enhanced opioid antinociception in inflammation. Naunyn-Schmiedeberg's Arch. Pharmacol. 342, 666-670 (1990)
'Involvement of capsaicin-sensitive neurones in hyperalgesia and enhanced opioid antinociception in inflammation ' () 342 Naunyn-Schmiedeberg's Arch. Pharmacol. : 666 -670.
De Oliveira JTA, Pusztai A, Grant G: Changes in organs and tissues induced by feeding of purified kidney bean (Phaseolus vulgaris) lectins. Nutr. Res. 8, 943-947 (1988)
'Changes in organs and tissues induced by feeding of purified kidney bean (Phaseolus vulgaris) lectins ' () 8 Nutr. Res. : 943 -947.
Fehér E, Vajda J: Selective sympathetic denervation induced by 6-hydroxy-dopamine in the small intestine. Acta Morph. Acad. Sci. Hung. 24, 121-128 (1976)
'Selective sympathetic denervation induced by 6-hydroxy-dopamine in the small intestine ' () 24 Acta Morph. Acad. Sci. Hung. : 121 -128.
Pusztai A, Grant G, Brown DS, Ewen SWB, Bardocz S: Phaseolus vulgaris lectin induces growth and increases the polyamine content of rat small intestine in vivo. Med. Sci. Res. 16, 1283-1284 (1988)
'Phaseolus vulgaris lectin induces growth and increases the polyamine content of rat small intestine in vivo ' () 16 Med. Sci. Res. : 1283 -1284.
Pusztai A, Grant G, de Oliveira JTA: Local (gut) and systemic response to dietary lectins. IRCS Med. Sci. 14, 205-208 (1986)
'Local (gut) and systemic response to dietary lectins ' () 14 IRCS Med. Sci. : 205 -208.
Pusztai A, Greer F, Grant G: Specific uptake of dietary lectins into the systemic circulation of rats. Biochem. Soc. Transactions 17, 481-482 (1989)
'Specific uptake of dietary lectins into the systemic circulation of rats ' () 17 Biochem. Soc. Transactions : 481 -482.
Racké K, Reimann A, Schwörer H, Kilbinger H: Regulation of 5-HT release from enterochromaffin cells. Behavioural Brain Res. 73, 83-87 (1996)
'Regulation of 5-HT release from enterochromaffin cells ' () 73 Behavioural Brain Res. : 83 -87.
Schwert GW, Takenaka Y: A spectrophotometric determination of trypsin and chymotrypsin. Biochim. Biophys. Acta 16, 570-575 (1955)
'A spectrophotometric determination of trypsin and chymotrypsin ' () 16 Biochim. Biophys. Acta : 570 -575.
Volant K, Grishina O, Descroix-Vagne M, Pansu D: Guanylin- heat-stable enterotoxin of Escherichia coli- and vasoactive intestinal peptide-induced water and ion secretion in the rat intestine in vivo. Eur. J. Pharmacol. 328, 217-227 (1997)
'Guanylin- heat-stable enterotoxin of Escherichia coli- and vasoactive intestinal peptide-induced water and ion secretion in the rat intestine in vivo ' () 328 Eur. J. Pharmacol. : 217 -227.