View More View Less
  • 1 New York Medical College Department of Medicine and Renal Research Institute BSB C-23 Valhalla NY 10595 USA
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $752.00

This brief overview of the potential diagnostic, prognostic and pathophysiological value of studies into the urine proteome describes hypothesis-driven investigations of individual proteins and proteome-wide search for urinary biomarkers of various diseases and their progression. It is intended to illustrate the recent progress in the area of urine proteomics and proselytize for the promise of this centuries-old technique of uroscopy, yet to reveal its secrets, using modern approaches.

  • Knepper MA: Proteomics and the kidney. J. Am. Soc. Nephrol. 13, 1398–1408 (2002)

    Knepper M.A. , 'Proteomics and the kidney ' (2002 ) 13 J. Am. Soc. Nephrol. : 1398 -1408.

  • Aebersold R, Mann M: Mass spectrometry-based proteomics. Nature 422, 198–207 (2003)

    Mann M. , 'Mass spectrometry-based proteomics ' (2003 ) 422 Nature : 198 -207.

  • Hewitt SM, Dear J, Star RA: Discovery of protein biomarkers for renal diseases. J. Am. Soc. Nephrol. 15, 1677–1689 (2004)

    Star R.A. , 'Discovery of protein biomarkers for renal diseases ' (2004 ) 15 J. Am. Soc. Nephrol. : 1677 -1689.

    • Search Google Scholar
  • Anderson NL, Polansky M, Pieper R, Gatlin T, Tirumalai RS, Conrads TP, Veenstra TD, Adkins JN, Pounds JG, Fagan R, Lobley A: The human plasma proteome: a nonredundant list developed by combination of four separate sources. Mol. Cell. Proteomics 3, 311–326 (2004)

    Lobley A. , 'The human plasma proteome: a nonredundant list developed by combination of four separate sources ' (2004 ) 3 Mol. Cell. Proteomics : 311 -326.

    • Search Google Scholar
  • Anderson NG, Anderson NL, Tollaksen SL: Proteins of human urine. I. Concentration and analysis by two-dimensional electrophoresis. Clin. Chem. 25, 1199–1210 (1979)

    Tollaksen S.L. , 'Proteins of human urine. I. Concentration and analysis by two-dimensional electrophoresis ' (1979 ) 25 Clin. Chem. : 1199 -1210.

    • Search Google Scholar
  • Pieper R et al.: Characterization of the human urinary proteome: a method for high-resolution display of urinary proteins on two-dimensional electrophoresis gels with a yield of nearly 1400 distinct protein spots. Proteomics 4, 1159–1174 (2004)

    Pieper R. , 'Characterization of the human urinary proteome: a method for high-resolution display of urinary proteins on two-dimensional electrophoresis gels with a yield of nearly 1400 distinct protein spots ' (2004 ) 4 Proteomics : 1159 -1174.

    • Search Google Scholar
  • Edwards JJ, Tollaksen SL, Anderson NG: Proteins of human urine. III. Identification and two-dimensional electrophoretic map positions of some major urinary proteins. Clin. Chem. 28, 941–948 (1982)

    Anderson N.G. , 'Proteins of human urine. III. Identification and two-dimensional electrophoretic map positions of some major urinary proteins ' (1982 ) 28 Clin. Chem. : 941 -948.

    • Search Google Scholar
  • Shaub S, Wilkins J, Weiler T, Sangster K, Rush D, Nickerson P: Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry. Kidney Int. 65, 323–332 (2004)

    Nickerson P. , 'Urine protein profiling with surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry ' (2004 ) 65 Kidney Int. : 323 -332.

    • Search Google Scholar
  • Riordan EO’, Orlova TN, Mei JJ, Butt K, Chander PM, Rahman S, Mya M, HU R, Momin J, Eng EW, Hampel DJ, Hartman B, Kretzler M, Delaney V, Goligorsky MS: Bioinformatic analysis of the urine proteome of acute allograft rejection. J. Am. Soc. Nephrol. 15, 3240–3248 (2004)

    Goligorsky M.S. , 'Bioinformatic analysis of the urine proteome of acute allograft rejection ' (2004 ) 15 J. Am. Soc. Nephrol. : 3240 -3248.

    • Search Google Scholar
  • Schaub S, Wilkins JA, Antonovici M, Krokhin O, Weiler T, Rush D, Nickerson P: Proteomic-based identification of cleaved urinary beta2-microglobulin as a potential marker for acute tubular injury in renal allografts. Am. J. Transplant. 5, 729–738 (2005)

    Nickerson P. , 'Proteomic-based identification of cleaved urinary beta2-microglobulin as a potential marker for acute tubular injury in renal allografts ' (2005 ) 5 Am. J. Transplant. : 729 -738.

    • Search Google Scholar
  • Hartmann H, Braedel H, Jutzler G: Detection of renal tubular lesions after abdominal aortography and selective renal arteriography by quantitative measurements of brush-border enzymes in the urine. Nephron 39, 95–101 (1985)

    Jutzler G. , 'Detection of renal tubular lesions after abdominal aortography and selective renal arteriography by quantitative measurements of brush-border enzymes in the urine ' (1985 ) 39 Nephron : 95 -101.

    • Search Google Scholar
  • Chew S, Lins R, Daelmans R, Nuyts G, DeBroe M: Urinary enzymes in acute renal failure. Nephrol. Dial. Transplant. 8, 507–511 (1993)

    DeBroe M. , 'Urinary enzymes in acute renal failure ' (1993 ) 8 Nephrol. Dial. Transplant. : 507 -511.

    • Search Google Scholar
  • Westhuyzen J, Endre Z, Reece G, Reith D, Saltissi D, Morgan T: Measurement of tubular enzymuria facilitates early detection of acute renal impairment in the intensive care unit. Nephrol. Dial. Transplant. 18, 543–551 (2003)

    Morgan T. , 'Measurement of tubular enzymuria facilitates early detection of acute renal impairment in the intensive care unit ' (2003 ) 18 Nephrol. Dial. Transplant. : 543 -551.

    • Search Google Scholar
  • Westhuizen J, Cochrane A, Tesar P, Mau T, Fleming S: Effect of supplementation with antioxidants (α-tocopherol and ascorbic acid) on markers of renal tubular injury in cardiac surgery patients. Nephrology 3, 535–539 (1997)

    Fleming S. , 'Effect of supplementation with antioxidants (α-tocopherol and ascorbic acid) on markers of renal tubular injury in cardiac surgery patients ' (1997 ) 3 Nephrology : 535 -539.

    • Search Google Scholar
  • Mishra J, Dent C, Tarabishi R, Mitsnefes M, Ma Q, Kelly C, Ruff S, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P: Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 365, 1231–1238 (2005)

    Devarajan P. , 'Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery ' (2005 ) 365 Lancet : 1231 -1238.

    • Search Google Scholar
  • Parikh C, Abraham E, Ancukiewicz M, Edelstein C: Urine IL-18 is an early diagnostic marker for acute kidney injury and predicts mortality in the intensive care unit. J. Am. Soc. Nephrol. 16, 3046–3052 (2005)

    Edelstein C. , 'Urine IL-18 is an early diagnostic marker for acute kidney injury and predicts mortality in the intensive care unit ' (2005 ) 16 J. Am. Soc. Nephrol. : 3046 -3052.

    • Search Google Scholar
  • Muramatsu Y, Tsujie M, Kohda Y, Pham B, Perantoni A, Zhao H, Jo S, Yuen PL, Craig, Hu X, Star R: Early detection of cysteine-rich protein 61 in urine following renal ischemic reperfusion injury. Kidney Internat. 62, 1601–1610 (2002)

    Star R. , 'Early detection of cysteine-rich protein 61 in urine following renal ischemic reperfusion injury ' (2002 ) 62 Kidney Internat. : 1601 -1610.

    • Search Google Scholar
  • Ichimura T, Bonventre J, Bailly V: Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is upregulated in renal cells after injury. J. Biol. Chem. 273, 4135–4142 (1998)

    Bailly V. , 'Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is upregulated in renal cells after injury ' (1998 ) 273 J. Biol. Chem. : 4135 -4142.

    • Search Google Scholar
  • Han W, Bailly V, Abichandani R, Thadhani R, Bonventre J: Kidney injury molecule-1 (KIM-1): a novel biomarker for human renal proximal tubule injury. Kidney Internat. 62, 237–244 (2002)

    Bonventre J. , 'Kidney injury molecule-1 (KIM-1): a novel biomarker for human renal proximal tubule injury ' (2002 ) 62 Kidney Internat. : 237 -244.

    • Search Google Scholar
  • Oh J et al.: Establishment of a near-standard two-dimensional human urine proteomic map. Proteomics 4, 3485–3497 (2004)

    Oh J. , 'Establishment of a near-standard two-dimensional human urine proteomic map ' (2004 ) 4 Proteomics : 3485 -3497.

    • Search Google Scholar
  • Smith G, Barratt D, Rowlinson R, Nickson J, Tonge R: Development of a high-throughput method for preparing human urine for two-dimensional electrophoresis. Proteomics 5(9), 2315–2318 (2005)

    Tonge R. , 'Development of a high-throughput method for preparing human urine for two-dimensional electrophoresis ' (2005 ) 5 Proteomics : 2315 -2318.

    • Search Google Scholar
  • Spahr CS et al.: Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry I. Profiling an unfractionated tryptic digest. Proteomics 1, 93–107 (2001)

    Spahr C.S. , 'Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry I. Profiling an unfractionated tryptic digest ' (2001 ) 1 Proteomics : 93 -107.

    • Search Google Scholar
  • Pisitkun T, Shen RF, Knepper MA: Identification and proteomic profiling of exosomes in human urine. Proc. Natl. Acad. Sci. USA 101, 13368–13373 (2004)

    Knepper M.A. , 'Identification and proteomic profiling of exosomes in human urine ' (2004 ) 101 Proc. Natl. Acad. Sci. USA : 13368 -13373.

    • Search Google Scholar
  • Norden AG et al.: Quantitative amino acid and proteomic analysis: very low excretion of polypeptides >750 Da in normal urine. Kidney Int. 66, 1994–2003 (2004)

    Norden A.G. , 'Quantitative amino acid and proteomic analysis: very low excretion of polypeptides >750 Da in normal urine ' (2004 ) 66 Kidney Int. : 1994 -2003.

    • Search Google Scholar
  • Cutillas PR et al.: The urinary proteome in Fanconi syndrome implies specificity in the reabsorption of proteins by renal proximal tubule cells. Am. J. Physiol. Renal Physiol. 287, F353–364 (2004)

    Cutillas P.R. , 'The urinary proteome in Fanconi syndrome implies specificity in the reabsorption of proteins by renal proximal tubule cells ' (2004 ) 287 Am. J. Physiol. Renal Physiol. : F353 -364.

    • Search Google Scholar
  • Clarke W et al.: Characterization of renal allograft rejection by urinary proteomic analysis. Ann. Surg. 237, 664–665 (2003)

    Clarke W. , 'Characterization of renal allograft rejection by urinary proteomic analysis ' (2003 ) 237 Ann. Surg. : 664 -665.

    • Search Google Scholar
  • Schaub S et al.: Proteomic-based detection of urine proteins associated with acute renal allograft rejection. J. Am. Soc. Nephrol. 15, 219–227 (2004)

    Schaub S. , 'Proteomic-based detection of urine proteins associated with acute renal allograft rejection ' (2004 ) 15 J. Am. Soc. Nephrol. : 219 -227.

    • Search Google Scholar
  • Weissinger EM, Wittke S, Kaiser T, Haller H, Bartel S, Krebs R, Golovko I, Rupprecht HD, Haubitz M, Hecker H, Mischak H, Fliser D: Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes. Kidney Int. 65, 2426–2434 (2004)

    Fliser D. , 'Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes ' (2004 ) 65 Kidney Int. : 2426 -2434.

    • Search Google Scholar
  • Mischak H et al.: Proteomic analysis for the assessment of diabetic renal damage in humans. Clin. Sci. (Lond.) 107, 485–495 (2004)

    Mischak H. , 'Proteomic analysis for the assessment of diabetic renal damage in humans ' (2004 ) 107 Clin. Sci. (Lond.) : 485 -495.

    • Search Google Scholar
  • Haubitz M et al.: Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy. Kidney Int. 67, 2313–2320 (2005)

    Haubitz M. , 'Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy ' (2005 ) 67 Kidney Int. : 2313 -2320.

    • Search Google Scholar
  • Hampel DJ et al.: Toward proteomics in uroscopy: urinary protein profiles after radiocontrast medium administration. J. Am. Soc. Nephrol. 12, 1026–1035 (2001)

    Hampel D.J. , 'Toward proteomics in uroscopy: urinary protein profiles after radiocontrast medium administration ' (2001 ) 12 J. Am. Soc. Nephrol. : 1026 -1035.

    • Search Google Scholar
  • Rogers MA et al.: Proteomic profiling of urinary proteins in renal cancer by surface enhanced laser desorption ionization and neural-network analysis: identification of key issues affecting potential clinical utility. Cancer Res. 63, 6971–6983 (2003)

    Rogers M.A. , 'Proteomic profiling of urinary proteins in renal cancer by surface enhanced laser desorption ionization and neural-network analysis: identification of key issues affecting potential clinical utility ' (2003 ) 63 Cancer Res. : 6971 -6983.

    • Search Google Scholar
  • Iwaki H et al.: Diagnostic potential in bladder cancer of a panel of tumor markers (calreticulin, gamma-synuclein, and catechol-o-methyltransferase) identified by proteomic analysis. Cancer Sci. 95, 955–961 (2004)

    Iwaki H. , 'Diagnostic potential in bladder cancer of a panel of tumor markers (calreticulin, gamma-synuclein, and catechol-o-methyltransferase) identified by proteomic analysis ' (2004 ) 95 Cancer Sci. : 955 -961.

    • Search Google Scholar
  • Kanno K, Sasaki S, Hirata Y, Ishikava S, Fushimi K, Nakanishi S, Bichet DG, Marumo F: Urinary excretion of aquaporin-2 in patients with diabetes insipidus. N. Engl. J. Med. 332(23), 1540–1545 (1995)

    Marumo F. , 'Urinary excretion of aquaporin-2 in patients with diabetes insipidus ' (1995 ) 332 N. Engl. J. Med. : 1540 -1545.

    • Search Google Scholar
  • Rai T, Sekine K, Kanno K, Hata K, Miura M, Mizushima A, Marumo F, Sasaki S: Urinary excretion of aquaporin-2 water channel protein in human and rat. J. Am. Soc. Nephrol. 8(9), 1357–1362 (1997)

    Sasaki S. , 'Urinary excretion of aquaporin-2 water channel protein in human and rat ' (1997 ) 8 J. Am. Soc. Nephrol. : 1357 -1362.

    • Search Google Scholar
  • Deen PM, Croes H, van Aubel RA, Ginsel LA, van Os CH: Water channels encoded by mutant aquaporin-2 genes in nephrogenic diabetes insipidus are impaired in their cellular routing. J. Clin. Invest. 95(5), 2291–2296 (1995)

    Os C.H. , 'Water channels encoded by mutant aquaporin-2 genes in nephrogenic diabetes insipidus are impaired in their cellular routing ' (1995 ) 95 J. Clin. Invest. : 2291 -2296.

    • Search Google Scholar
  • Noda Y, Sasaki S: Molecular mechanisms and drug development in aquaporin water channel diseases: molecular mechanism of water channel aquaporin-2 trafficking. J. Pharmacol. Sci. 96(3), 249–254 (2004)

    Sasaki S. , 'Molecular mechanisms and drug development in aquaporin water channel diseases: molecular mechanism of water channel aquaporin-2 trafficking ' (2004 ) 96 J. Pharmacol. Sci. : 249 -254.

    • Search Google Scholar
  • Causes of reversible nephrogenic diabetes insipidus: a systematic review. Am. J. Kidney Dis. 45, 626–637 (2005)

  • Murer LL, Addabbo F, Carmosino M, Procino G, Tamma G, Montini G, Rigamonti W, Zucchetta P, Della Vella M, Venturini A, Zacchello G, Svelto M, Valenti G: Selective decrease in urinary aquaporin 2 and increase in prostaglandin E2 excretion is associated with postobstructive polyuria in human congenital hydronephrosis, J. Am. Soc. Nephrol. 15, 2705–2712 (2004)

    Valenti G. , 'Selective decrease in urinary aquaporin 2 and increase in prostaglandin E2 excretion is associated with postobstructive polyuria in human congenital hydronephrosis ' (2004 ) 15 J. Am. Soc. Nephrol. : 2705 -2712.

    • Search Google Scholar
  • Pedersen RS, Bentze H, Bech JN, Nyvad O, Pedersen EB: Urinary aquaporin-2 in healthy humans and patients with liver cirrhosis and chronic heart failure during baseline conditions and after acute water load. Kidney Int. 63(4), 1417–1425 (2003)

    Pedersen E.B. , 'Urinary aquaporin-2 in healthy humans and patients with liver cirrhosis and chronic heart failure during baseline conditions and after acute water load ' (2003 ) 63 Kidney Int. : 1417 -1425.

    • Search Google Scholar
  • Xu DL, Martin PY, Ohara M, St John J, Pattison T, Meng X, Morris K, Kim JK, Schrier R: Upregulation of aquaporin-2 water channel expression in chronic heart failure rat. J. Clin. Invest. 99(7), 1500–1505 (1997)

    Schrier R. , 'Upregulation of aquaporin-2 water channel expression in chronic heart failure rat ' (1997 ) 99 J. Clin. Invest. : 1500 -1505.

    • Search Google Scholar
  • Valenti G, Laera A, Pace G, Aceto G, Lospalluti ML, Penza R, Selvaggi FP, Chiozza ML, Svelto M: Urinary aquaporin 2 and calciuria correlate with the severity of enuresis in children. J. Am. Soc. Nephrol. 11(10), 1873–1881 (2000)

    Svelto M. , 'Urinary aquaporin 2 and calciuria correlate with the severity of enuresis in children ' (2000 ) 11 J. Am. Soc. Nephrol. : 1873 -1881.

    • Search Google Scholar
  • Umenishi F, Summer SN, Cadnaphornchai M, Schrier RW: Comparison of three methods to quantify urinary aquaporin-2 protein. Kidney Int. 62, 2288–2293 (2002)

    Schrier R.W. , 'Comparison of three methods to quantify urinary aquaporin-2 protein ' (2002 ) 62 Kidney Int. : 2288 -2293.

    • Search Google Scholar
  • Valenti G, Fraszl W, Addabbo F, Tamma G, Procino G, Satta E, Cirillo M, De Santo NG, Drummer C, Bellini L, Kowoll R, Schlemmer M, Vogler S, Kirsch KA, Sveto M, Gunga HC: Water immersion is associated with an increase in aquaporin-2 excretion in healthy volunteers. Biochim. Biophys. Acta. Apr 24; PMID: 129, 44406 (2006)

    Gunga H.C. , 'Water immersion is associated with an increase in aquaporin-2 excretion in healthy volunteers ' (2006 ) 24 Biochim. Biophys. Acta. Apr : 44406 -.

    • Search Google Scholar
  • Weissinger EM et al.: Proteomics: a novel tool to unravel the patho-physiology of uraemia. Nephrol. Dial. Transplant. 19, 3068–3077 (2004)

    Weissinger E.M. , 'Proteomics: a novel tool to unravel the patho-physiology of uraemia ' (2004 ) 19 Nephrol. Dial. Transplant. : 3068 -3077.

    • Search Google Scholar
  • Molina H et al.: A proteomic analysis of human hemodialysis fluid. Mol. Cell. Proteomics 4, 637–650 (2005)

    Molina H. , 'A proteomic analysis of human hemodialysis fluid ' (2005 ) 4 Mol. Cell. Proteomics : 637 -650.

    • Search Google Scholar
  • Thongboonkerd V et al.: Alterations in the renal elastin-elastase system in type 1 diabetic nephropathy identified by proteomic analysis. J. Am. Soc. Nephrol. 15, 650–662 (2004)

    Thongboonkerd V. , 'Alterations in the renal elastin-elastase system in type 1 diabetic nephropathy identified by proteomic analysis ' (2004 ) 15 J. Am. Soc. Nephrol. : 650 -662.

    • Search Google Scholar
  • Kwapiszewska G et al.: Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS. BMC Biotechnol. 4, 30 (2004)

    Kwapiszewska G. , 'Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS ' (2004 ) 4 BMC Biotechnol. : 30 -.

    • Search Google Scholar
  • Chaurand P, Sanders ME, Jensen RA, Caprioli RM: Proteomics in diagnostic pathology: profiling and imaging proteins directly in tissue sections. Am. J. Pathol. 165, 1057–1068 (2004)

    Caprioli R.M. , 'Proteomics in diagnostic pathology: profiling and imaging proteins directly in tissue sections ' (2004 ) 165 Am. J. Pathol. : 1057 -1068.

    • Search Google Scholar
  • Brookes PS, Pinner A, Ramachandran A, Coward L, Barnes S, Kim H, Darley-Usmar VM: High throughput two-dimensional blue-native electrophoresis: a tool for functional proteomics of mitochondria and signaling complexes. Proteomics 2, 969–977 (2002)

    Darley-Usmar V.M. , 'High throughput two-dimensional blue-native electrophoresis: a tool for functional proteomics of mitochondria and signaling complexes ' (2002 ) 2 Proteomics : 969 -977.

    • Search Google Scholar
  • Bagshaw RD, Mahuran DJ, Callahan JW: A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle. Mol. Cell Proteomics 4, 133–143 (2005)

    Callahan J.W. , 'A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle ' (2005 ) 4 Mol. Cell Proteomics : 133 -143.

    • Search Google Scholar
  • O’Riordan E, Goligorsky MS: Emerging studies of the urinary proteome: the end of the beginning? Curr. Opin. Nephrol. Hypertens. 14(6), 579–585 (2005)

    Goligorsky M.S. , 'Emerging studies of the urinary proteome: the end of the beginning? ' (2005 ) 14 Curr. Opin. Nephrol. Hypertens. : 579 -585.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2020 1 4 0
Dec 2020 3 1 1
Jan 2021 0 0 0
Feb 2021 3 0 0
Mar 2021 4 0 0
Apr 2021 0 0 0
May 2021 0 0 0