A number of human diseases and pathological conditions were found to be associated with increased oxidative stress. In the literature several techniques are available for the assessment of oxidative stress, but most of them are not applicable for a routine medical laboratory due to the complex methodology and/or financial reasons. We report here on a simple, inexpensive, kinetic assay for the determination of the oxidative stress biomarker, advanced oxidation protein products (AOPP) in the human blood plasma.
This study involved 70 patients (47M/23F; mean age: 64.6 y; range: 16–85) admitted to our Department with a wide range of cardiovascular and peripheral vascular diseases. Three critically ill patients were assigned for monitoring purposes. Plasma AOPP were simultaneously determined using an end-point assay as reference method and by a kinetic method developed in our laboratory. Plasma fibrinogen concentration was measured according to the Clauss method.
There was a highly significant correlation (r
<0.0001) between AOPP concentration (reference method) and AOPP reactivity (kinetic method). Both AOPP concentration and AOPP reactivity also significantly correlated with plasma fibrinogen concentration (r
<0.0001). The three representative cases presented appear to support the relevance of our novel method in the monitoring of critically ill patients.
This simple and inexpensive kinetic assay can be widely used in any routine laboratory interested in oxidative stress research. It is especially recommended for monitoring critically ill or other patients.
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Kahan A.Acute and sustained effects of dihydropyridine-type calcium channel antagonists on oxidative stress in systemic sclerosisAm. J. Med.2004116595600)| false
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Dominguez C.Biomarkers of diabetes — associated oxidative stress and antioxidant status in young diabetic patients with or without subclinical complicationsFree Radic. Biol. Med.20033415631574)| false
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Acsády G.Advanced oxidation protein products (AOPP) for monitoring oxidative stress in critically ill patients: a simple, fast and inexpensive automated techniqueClin. Chem. Lab. Med.200543294297)| false
Valli A, Suliman ME, Meert N, Vanholder R, Lindholm B, Stenvinkel P, Watanabe M, Barany P, Alvestrand A, Anderstam B: Overestimation of advanced oxidation protein products in uremic plasma due to the presence of triglycerides and other endogenous factors. Clin. Chim. Acta 379, 87–94 (2007)
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Anderstam B.Overestimation of advanced oxidation protein products in uremic plasma due to the presence of triglycerides and other endogenous factorsClin. Chim. Acta20073798794)| false
Co-editor(s): Koller, Ákos; Lénárd, László; Szénási, Gábor
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Benedek, György (Szeged)Benyó, Zoltán (Budapest)Boros, Mihály (Szeged)Chernoch, László (Debrecen)Détári, László (Budapest)Hamar, János (Budapest)Hantos, Zoltán (Szeged)Hunyady, László (Budapest)Imre, Sándor (Debrecen)Jancsó, Gábor (Szeged)Karádi, Zoltán (Pécs)Kovács, László (Debrecen)Palkovits, Miklós (Budapest)Papp, Gyula (Szeged)Pavlik, Gábor (Budapest)Spät, András (Budapest)Szabó, Gyula (Szeged)Szelényi, Zoltán (Pécs)Szolcsányi, János (Pécs)Szollár, Lajos (Budapest)Szücs, Géza (Debrecen)Telegdy, Gyula (Szeged)Toldi, József (Szeged)Tósaki, Árpád (Debrecen)
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