Acta Physiologica Hungarica
Volume/Issue:
Volume 96: Issue 2
Co-administration of nerve growth factor and butyrate regulates vanilloid receptor 1 and substance P levels in cultures of rat dorsal root ganglion neurons
Authors:
Z. Liu Shandong University School of Medicine Department of Anatomy Jinan 250012 China

Search for other papers by Z. Liu in
Current site
Google Scholar
PubMed Close
,
F. Zhang Shandong University School of Medicine Department of Anatomy Jinan 250012 China

Search for other papers by F. Zhang in
Current site
Google Scholar
PubMed Close
,
H. Liu Shandong University Qilu Hospital Department of Rheumatology Jinan 250012 China

Search for other papers by H. Liu in
Current site
Google Scholar
PubMed Close
,
X. Yang Shandong University Qilu Hospital Department of Nephrology Jinan 250012 China

Search for other papers by X. Yang in
Current site
Google Scholar
PubMed Close
,
H. Wang Shandong University School of Medicine Department of Anatomy Jinan 250012 China

Search for other papers by H. Wang in
Current site
Google Scholar
PubMed Close
, and
Zhenzhong Li Shandong University School of Medicine Department of Anatomy Jinan 250012 China

Search for other papers by Zhenzhong Li in
Current site
Google Scholar
PubMed Close
Pages:
231–241
Online Publication Date:
12 May 2009
Publication Date:
01 Jun 2009
Article Category:
Journal Article
DOI:
https://doi.org/10.1556/aphysiol.96.2009.2.8
Keywords:
nerve growth factor; butyrate; vanilloid receptor 1; substance P; capsaicin; dorsal root ganglion; Wistar rat
Restricted access

The aim of the present study was to investigate whether co-administration of nerve growth factor (NGF) and butyrate regulates vanilloid receptor 1 (VR1) and substance P (SP) levels in cultures of rat dorsal root ganglion (DRG) neurons. DRG was dissected out from embryonic 15-day-old Wistar rat and cultured as dissociated cells for 2 days then exposed to NGF (10 ng/ml), butyrate (1 mmol/L), NGF (10 ng/ml) plus butyrate (1 mmol/L) for another 4 days. The neurons cultured continuously in media served as normal control. After that, the cultures were processed for detecting expression of mRNA for VR1 and SP in DRG neurons by RT-PCR, and expression of VR1 protein by Western blot. SP basal release levels were measured by radioimmunoassay (RIA). Capsaicin-evoked SP release was measured by RIA after stimulation with capsaicin (100 nmol/L) for 10 minutes. The neurons exposed to vehicle solution served as vehicle control. Either NGF (10 ng/ml) or butyrate (1 mmol/L) promoted expression of SP mRNA, VR1 mRNA, and VR1 protein in DRG neurons and capsaicin-evoked SP release from DRG neurons. Co-administration of NGF and butyrate showed a synergistic effect on expression of VR1 mRNA, and VR1 protein in DRG neurons and capsaicin-evoked SP release from DRG neurons and a ceiling effect on SP mRNA expression. The elevation of SP mRNA, VR1 mRNA, and VR1 protein promoted by NGF and/or butyrate may be associated with increases of SP release evoked by capsaicin. The mechanisms of the effects of co-administration of NGF and butyrate should be clarified by further study.

  • Collapse
  • Expand
Cover Acta Physiologica Hungarica
Acta Physiologica Hungarica
Print ISSN:
0231-424X
Online ISSN:
1588-2683

Senior editors

Editor(s)-in-Chief: Rosivall, László

Honorary Editor(s)-in-Chief): Monos, Emil

Managing Editor(s): Bartha, Jenő; Berhidi, Anna

Co-editor(s): Koller, Ákos; Lénárd, László; Szénási, Gábor

Assistant Editor(s): G. Dörnyei (Budapest), Zs. Miklós (Budapest), Gy. Nádasy (Budapest)

Hungarian Editorial Board

    1. Benedek, György (Szeged)
    1. Benyó, Zoltán (Budapest)
    1. Boros, Mihály (Szeged)
    1. Chernoch, László (Debrecen)
    1. Détári, László (Budapest)
    1. Hamar, János (Budapest)
    1. Hantos, Zoltán (Szeged)
    1. Hunyady, László (Budapest)
    1. Imre, Sándor (Debrecen)
    1. Jancsó, Gábor (Szeged)
    1. Karádi, Zoltán (Pécs)
    1. Kovács, László (Debrecen)
    1. Palkovits, Miklós (Budapest)
    1. Papp, Gyula (Szeged)
    1. Pavlik, Gábor (Budapest)
    1. Spät, András (Budapest)
    1. Szabó, Gyula (Szeged)
    1. Szelényi, Zoltán (Pécs)
    1. Szolcsányi, János (Pécs)
    1. Szollár, Lajos (Budapest)
    1. Szücs, Géza (Debrecen)
    1. Telegdy, Gyula (Szeged)
    1. Toldi, József (Szeged)
    1. Tósaki, Árpád (Debrecen)

International Editorial Board

    1. R. Bauer (Jena)
    1. W. Benjelloun (Rabat)
    1. A. W. Cowley Jr. (Milwaukee)
    1. D. Djuric (Belgrade)
    1. C. Fry (London)
    1. S. Greenwald (London)
    1. O. Hänninen (Kuopio)
    1. H. G. Hinghofer-Szalkay (Graz)
    1. Th. Kenner (Graz)
    1. Gy. Kunos (Richmond)
    1. M. Mahmoudian (Tehran)
    1. T. Mano (Seki, Gifu)
    1. G. Navar (New Orleans)
    1. H. Nishino (Nagoya)
    1. O. Petersen (Liverpool)
    1. U. Pohl (Münich)
    1. R. S. Reneman (Maastricht)
    1. A. Romanovsky (Phoenix)
    1. G. M. Rubanyi (Richmond)
    1. T. Sakata (Oita)
    1. A. Siddiqui (Karachi)
    1. Cs. Szabo (Beverly)
    1. E. Vicaut (Paris)
    1. N. Westerhof (Amsterdam)
    1. L. F. Zhang (Xi'an)

Editorial Office:
Akadémiai Kiadó Zrt.
Prielle Kornélia u. 21–35, H-1117 Budapest, Hungary

Editorial Correspondence:
Acta Physiologica Hungarica
Semmelweis University, Faculty of Medicine Institute of Pathophysiology
Nagyvárad tér 4, H-1089 Budapest, Hungary
Phone/Fax: +36-1-2100-100
E-mail: aph@semmelweis-univ.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • EMBASE/Excerpta Medica
  • Global Health
  • Index Copernicus
  • Index Medicus
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index Expanded
  • SCOPUS

 

Acta Physiologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0231-424X (Print)
ISSN 1588-2683 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 26 0 0
Dec 2024 11 0 0
Jan 2025 19 0 0
Feb 2025 35 0 0
Mar 2025 45 0 0
Apr 2025 11 0 0
May 2025 4 0 0