Acta Physiologica Hungarica
Volume/Issue:
Volume 98: Issue 3
Exercise changes volatiles in exhaled breath assessed by an electronic nose
Authors: A. Bikov 1 , Zs Lazar 1 , K. Schandl 2 , B. Antus 3 , G. Losonczy 1 , and Ildiko Horvath
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  • 1 Semmelweis University Department of Pulmonology Budapest Hungary
  • 2 Semmelweis University Institute of Human Physiology and Clinical Experimental Research Budapest Hungary
  • 3 National Korányi Institute for TB and Pulmonology Department of Pathophysiology Budapest Hungary
  • 4 Diósárok u. 1/C H-1125 Budapest Hungary
DOI:
https://doi.org/10.1556/aphysiol.98.2011.3.9
Page Count:
321–328
Publication Date:
01 Sep 2011
Online Publication Date:
05 Sep 2011
Article Category:
Research Article
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Exercise-caused metabolic changes can be followed by monitoring exhaled volatiles; however it has not been previously reported if a spectrum of exhaled gases is modified after physical challenge. We have hypothesized that changes in volatile molecules assessed by an electronic nose may be the reason for the alkalization of the exhaled breath condensate (EBC) fluid following physical exercise.Ten healthy young subjects performed a 6-minute running test. Exhaled breath samples pre-exercise and post-exercise (0 min, 15 min, 30 min and 60 min) were collected for volatile pattern (“smellprint”) determination and pH measurements (at 5.33 kPa CO2), respectively. Exhaled breath smellprints were analyzed using principal component analysis and were related to EBC pH.Smellprints (p=0.04) and EBC pH (p=0.01) were altered during exercise challenge. Compared to pre-exercise values, smellprints and pH differed at 15 min, 30 min and 60 min following exercise (p<0.05), while no difference was found at 0 min post-exercise. In addition, a significant correlation was found between volatile pattern of exhaled breath and EBC pH (p=0.01, r=−0.34).Physical exercise changes the pattern of exhaled volatiles together with an increase in pH of breath. Changes in volatiles may be responsible for increase in EBC pH.

  • Ament W, Huizenga JR, Mook GA, Gips CH, Verkerke GJ: Lactate and ammonia concentration in blood and sweat during incremental cycle ergometer exercise. Int. J. Sports Med. 18, 35–39 (1997)

    Verkerke G.J. , 'Lactate and ammonia concentration in blood and sweat during incremental cycle ergometer exercise ' (1997 ) 18 Int. J. Sports Med. : 35 -39.

    • Search Google Scholar

  • Araneda OF, Garcia C, Lagos N, Quiroga G, Cajigal J, Salazar MP, Behn C: Lung oxidative stress as related to exercise and altitude. Lipid peroxidation evidence in exhaled breath condensate: a possible predictor of acute mountain sickness. Eur. J. Appl. Physiol. 95, 383–390 (2005)

    Behn C. , 'Lung oxidative stress as related to exercise and altitude. Lipid peroxidation evidence in exhaled breath condensate: a possible predictor of acute mountain sickness ' (2005 ) 95 Eur. J. Appl. Physiol. : 383 -390.

    • Search Google Scholar

  • Barreto M, Villa MP, Olita C, Martella S, Ciabattoni G, Montuschi P: 8-Isoprostane in exhaled breath condensate and exercise-induced bronchoconstriction in asthmatic children and adolescents. Chest 135, 66–73 (2009)

    Montuschi P. , '8-Isoprostane in exhaled breath condensate and exercise-induced bronchoconstriction in asthmatic children and adolescents ' (2009 ) 135 Chest : 66 -73.

    • Search Google Scholar

  • Dalton P, Gelperin A, Preti G: Volatile metabolic monitoring of glycemic status in diabetes using electronic olfaction. Diabetes Technol. Ther. 6, 534–544 (2004)

    Preti G. , 'Volatile metabolic monitoring of glycemic status in diabetes using electronic olfaction ' (2004 ) 6 Diabetes Technol. Ther. : 534 -544.

    • Search Google Scholar

  • Dragonieri S, Schot R, Mertens BJ, Le Cessie S, Gauw SA, Spanevello A, Resta O, Willard NP, Vink TJ, Rabe KF, Bel EH, Sterk PJ: An electronic nose in the discrimination of patients with asthma and controls. J. Allergy Clin. Immunol. 120, 856–862 (2007)

    Sterk P.J. , 'An electronic nose in the discrimination of patients with asthma and controls ' (2007 ) 120 J. Allergy Clin. Immunol. : 856 -862.

    • Search Google Scholar

  • Effros RM, Casaburi R, Su J, Dunning M, Torday J, Biller J, Shaker R: The effects of volatile salivary acids and bases on exhaled breath condensate pH. Am. J. Respir. Crit. Care Med. 173, 386–392 (2006)

    Shaker R. , 'The effects of volatile salivary acids and bases on exhaled breath condensate pH ' (2006 ) 173 Am. J. Respir. Crit. Care Med. : 386 -392.

    • Search Google Scholar

  • Fens N, Zwinderman AH, van der Schee MP, de Nijs SB, Dijkers E, Roldaan AC, Cheung D, Bel EH, Sterk PJ: Exhaled breath profiling enables discrimination of chronic obstructive pulmonary disease and asthma. Am. J. Respir. Crit. Care Med. 180, 1076–1082 (2009)

    Sterk P.J. , 'Exhaled breath profiling enables discrimination of chronic obstructive pulmonary disease and asthma ' (2009 ) 180 Am. J. Respir. Crit. Care Med. : 1076 -1082.

    • Search Google Scholar

  • Greenwald R, Ferdinands JM, Teague WG: Ionic determinants of exhaled breath condensate pH before and after exercise in adolescent athletes. Pediatr. Pulmonol. 44, 768–777 (2009)

    Teague W.G. , 'Ionic determinants of exhaled breath condensate pH before and after exercise in adolescent athletes ' (2009 ) 44 Pediatr. Pulmonol. : 768 -777.

    • Search Google Scholar

  • Hay P, Tummon A, Ogunfile M, Adebiyi A, Adefowora A: Evaluation of a novel diagnostic test for bacterial vaginosis: ‘the electronic nose’. Int. J. STD AIDS. 14, 114–118 (2003)

    Adefowora A. , 'Evaluation of a novel diagnostic test for bacterial vaginosis: ‘the electronic nose’ ' (2003 ) 14 Int. J. STD AIDS. : 114 -118.

    • Search Google Scholar

  • Horvath I, Hunt J, Barnes PJ, Alving K, Antczak A, Baraldi E, Becher G, van Beurden WJ, Corradi M, Dekhuijzen R, Dweik RA, Dwyer T, Effros R, Erzurum S, Gaston B, Gessner C, Greening A, Ho LP, Hohlfeld J, Jobsis Q, Laskowski D, Loukides S, Marlin D, Montuschi P, Olin AC, Redington AE, Reinhold P, van Rensen EL, Rubinstein I, Silkoff P, Toren K, Vass G, Vogelberg C, Wirtz H: Exhaled breath condensate: methodological recommendations and unresolved questions. Eur. Respir. J. 26, 523–548 (2005)

    Wirtz H. , 'Exhaled breath condensate: methodological recommendations and unresolved questions ' (2005 ) 26 Eur. Respir. J. : 523 -548.

    • Search Google Scholar

  • Horvath I, Lazar Z, Gyulai N, Kollai M, Losonczy G: Exhaled biomarkers in lung cancer. Eur. Respir. J. 34, 261–275 (2009)

    Losonczy G. , 'Exhaled biomarkers in lung cancer ' (2009 ) 34 Eur. Respir. J. : 261 -275.

  • Hunt JF, Fang K, Malik R, Snyder A, Malhotra N, Platts-Mills TA, Gaston B: Endogenous airway acidification. Implications for asthma pathophysiology. Am. J. Respir. Crit. Care Med. 161, 694–699 (2000)

    Gaston B. , 'Endogenous airway acidification. Implications for asthma pathophysiology ' (2000 ) 161 Am. J. Respir. Crit. Care Med. : 694 -699.

    • Search Google Scholar

  • Hunt JF, Erwin E, Palmer L, Vaughan J, Malhotra N, Platts-Mills TA, Gaston B: Expression and activity of pH-regulatory glutaminase in the human airway epithelium. Am. J. Respir. Crit. Care Med. 165, 101–107 (2002)

    Gaston B. , 'Expression and activity of pH-regulatory glutaminase in the human airway epithelium ' (2002 ) 165 Am. J. Respir. Crit. Care Med. : 101 -107.

    • Search Google Scholar

  • Ji LL: Oxidative stress during exercise: implication of antioxidant nutrients. Free Radic. Biol. Med. 18, 1079–1086 (1995)

    Ji L.L. , 'Oxidative stress during exercise: implication of antioxidant nutrients ' (1995 ) 18 Free Radic. Biol. Med. : 1079 -1086.

    • Search Google Scholar

  • Ka T, Yamamoto T, Moriwaki Y, Kaya M, Tsujita J, Takahashi S, Tsutsumi Z, Fukuchi M, Hada T: Effect of exercise and beer on the plasma concentration and urinary excretion of purine bases. J. Rheumatol. 30, 1036–1042 (2003)

    Hada T. , 'Effect of exercise and beer on the plasma concentration and urinary excretion of purine bases ' (2003 ) 30 J. Rheumatol. : 1036 -1042.

    • Search Google Scholar

  • King J, Kupferthaler A, Unterkofler K, Koc H, Teschl S, Teschl G, Miekisch W, Schubert J, Hinterhuber H, Amann A: Isoprene and acetone concentration profiles during exercise on an ergometer. J. Breath Res. 3, 1–16 (2009)

    Amann A. , 'Isoprene and acetone concentration profiles during exercise on an ergometer ' (2009 ) 3 J. Breath Res. : 1 -16.

    • Search Google Scholar

  • Kondo M, Tamaoki J, Sakai A, Kameyama S, Kanoh S, Konno K: Increased oxidative metabolism in cow tracheal epithelial cells cultured at air-liquid interface. Am. J. Respir. Cell Mol. Biol. 16, 62–68 (1997)

    Konno K. , 'Increased oxidative metabolism in cow tracheal epithelial cells cultured at air-liquid interface ' (1997 ) 16 Am. J. Respir. Cell Mol. Biol. : 62 -68.

    • Search Google Scholar

  • Kullmann T, Barta I, Lazar Z, Szili B, Barat E, Valyon M, Kollai M, Horvath I: Exhaled breath condensate pH standardised for CO2 partial pressure Eur. Respir. J. 29, 496–501 (2007)

    Horvath I. , 'Exhaled breath condensate pH standardised for CO2 partial pressure Eur ' (2007 ) 29 Respir. J. : 496 -501.

    • Search Google Scholar

  • Machado RF, Laskowski D, Deffenderfer O, Burch T, Zheng S, Mazzone PJ, Mekhail T, Jennings C, Stoller JK, Pyle J, Duncan J, Dweik RA, Erzurum SC: Detection of lung cancer by sensor array analyses of exhaled breath. Am. J. Respir. Crit. Care Med. 171, 1286–1291 (2005)

    Erzurum S.C. , 'Detection of lung cancer by sensor array analyses of exhaled breath ' (2005 ) 171 Am. J. Respir. Crit. Care Med. : 1286 -1291.

    • Search Google Scholar

  • Meyer F, Laitano O, Bar-Or O, McDougall D, Heigenhauser GJ: Effect of age and gender on sweat lactate and ammonia concentrations during exercise in the heat. Braz. J. Med. Biol. Res. 40, 135–143 (2007)

    Heigenhauser G.J. , 'Effect of age and gender on sweat lactate and ammonia concentrations during exercise in the heat ' (2007 ) 40 Braz. J. Med. Biol. Res. : 135 -143.

    • Search Google Scholar

  • Miller MR, Hankinson J, Brusasco V, Burgos F, Casaburi R, Coates A, Crapo R, Enright P, van der Grinten CP, Gustafsson P, Jensen R, Johnson DC, MacIntyre N, McKay R, Navajas D, Pedersen OF, Pellegrino R, Viegi G, Wanger J: Standardisation of spirometry. Eur. Respir. J. 26, 319–338 (2005)

    Wanger J. , 'Standardisation of spirometry ' (2005 ) 26 Eur. Respir. J. : 319 -338.

  • Montuschi P, Santonico M, Pennazza G, Mondino C, Mantini G, Martinelli E, Capuano R, Ciabattoni G, Paolesse R, Di Natale C, Barnes PJ, D’Amico A: Diagnostic performance of an electronic nose, fractional exhaled nitric oxide and lung function testing in asthma. Chest 137, 790–796 (2010)

    D’Amico A. , 'Diagnostic performance of an electronic nose, fractional exhaled nitric oxide and lung function testing in asthma ' (2010 ) 137 Chest : 790 -796.

    • Search Google Scholar

  • Pavlou AK, Magan N, McNulty C, Jones J, Sharp D, Brown J, Turner AP: Use of an electronic nose system for diagnoses of urinary tract infections. Biosens. Bioelectron. 17, 893–899 (2002)

    Turner A.P. , 'Use of an electronic nose system for diagnoses of urinary tract infections ' (2002 ) 17 Biosens. Bioelectron. : 893 -899.

    • Search Google Scholar

  • Phillips M, Cataneo RN, Cummin AR, Gagliardi AJ, Gleeson K, Greenberg J, Maxfield RA, Rom WN: Detection of lung cancer with volatile markers in the breath. Chest 123, 2115–2123 (2003)

    Rom W.N. , 'Detection of lung cancer with volatile markers in the breath ' (2003 ) 123 Chest : 2115 -2123.

    • Search Google Scholar

  • Pucsok JM, Gyore I, Argay K, Huszar E, Barat E, Pucsok J, Horvath I: Effect of exercise on levels of cyclo-oxygenase mediators in exhaled breath condensate in elite athletes. J. Sports Med. Phys. Fitness 47, 223–227 (2007)

    Horvath I. , 'Effect of exercise on levels of cyclo-oxygenase mediators in exhaled breath condensate in elite athletes ' (2007 ) 47 J. Sports Med. Phys. Fitness : 223 -227.

    • Search Google Scholar

  • Riediker M, Danuser B: Exhaled breath condensate pH is increased after moderate exercise. J. Aerosol Med. 20, 13–18 (2007)

    Danuser B. , 'Exhaled breath condensate pH is increased after moderate exercise ' (2007 ) 20 J. Aerosol Med. : 13 -18.

    • Search Google Scholar

  • Shykhon ME, Morgan DW, Dutta R, Hines EL, Gardner JW: Clinical evaluation of the electronic nose in the diagnosis of ear, nose and throat infection: a preliminary study. J. Laryngol. Otol. 118, 706–709 (2004)

    Gardner J.W. , 'Clinical evaluation of the electronic nose in the diagnosis of ear, nose and throat infection: a preliminary study ' (2004 ) 118 J. Laryngol. Otol. : 706 -709.

    • Search Google Scholar

  • Thaler ER, Hanson CW: Use of an electronic nose to diagnose bacterial sinusitis. Am. J. Rhinol. 20, 170–172 (2006)

    Hanson C.W. , 'Use of an electronic nose to diagnose bacterial sinusitis ' (2006 ) 20 Am. J. Rhinol. : 170 -172.

    • Search Google Scholar

  • Viru M, Hackney AC, Karelson K, Janson T, Kuus M, Viru A: Competition effects on physiological responses to exercise: performance, cardiorespiratory and hormonal factors. Acta Physiol. Hung. 97, 22–30 (2010)

    Viru A. , 'Competition effects on physiological responses to exercise: performance, cardiorespiratory and hormonal factors ' (2010 ) 97 Acta Physiol. Hung. : 22 -30.

    • Search Google Scholar

  • Wells K, Vaughan J, Pajewski TN, Hom S, Ngamtrakulpanit L, Smith A, Nguyen A, Turner R, Hunt J: Exhaled breath condensate pH assays are not influenced by oral ammonia. Thorax. 60, 27–31 (2005)

    Hunt J. , 'Exhaled breath condensate pH assays are not influenced by oral ammonia ' (2005 ) 60 Thorax : 27 -31.

    • Search Google Scholar
Cover Acta Physiologica Hungarica
Acta Physiologica Hungarica
Print ISSN:
0231-424X
Online ISSN:
1588-2683
Keywords:
exercise; exhaled breath condensate; exhaled volatiles; healthy; pH; electronic nose

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