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  • 1 Semmelweis University Department of Orthopedics Budapest Hungary
  • 2 Semmelweis University Department of Human Physiology and Clinical Experimental Research Budapest Hungary
  • 3 Semmelweis University Department of Radiology, Faculty of Dentistry Budapest Hungary
  • 4 Péterfy Sándor Hospital Department of Pathology Budapest Hungary
  • 5 Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Vienna Austria
  • 6 Semmelweis University Nanobiotechnology and In Vivo Imaging Centre, Department of Biophysics and Radiation Biology Budapest Hungary
  • 7 CROmed Research Centers Ltd. Budapest Hungary
  • 8 Semmelweis University Dept. of Orthopedics Karolina út 27 H-1113 Budapest Hungary
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Purpose: The clinical demand for bone grafting materials necessitated the development of animal models. Critical size defect model has been criticized recently, mainly for its inaccuracy. Our objective was to develop a dependable animal model that would provide compromised bone healing, and would allow the investigation of bone substitutes. Methods: In the first group a critical size defect was created in the femur of adult male Wistar rats, and a non-critical defect in the remaining animals (Groups II, III and IV). The defect was left empty in group II, while in groups III and IV a spacer was interposed into the gap. Osteoblast activity was evaluated by NanoSPECT/CT imaging system. New bone formation and assessment of a union or non-union was observed by μCT and histology. Results: The interposition model proved to be highly reproducible and provided a bone defect with compromised bone healing. Significant bone regeneration processes were observed four weeks after removal of the spacer. Conclusion: Our results have shown that when early bone healing is inhibited by the physical interposition of a spacer, the regeneration process is compromised for a further 4 weeks and results in a bone defect during the time-course of the study.

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