Acta Physiologica Hungarica
Volume/Issue:
Volume 99: Issue 3
Hypoxia-inducible factor 1 mediates the anti-apoptosis of berberine in neurons during hypoxia/ischemia
Authors: Q. Zhang 1 , Z. Qian 1 , L. Pan 1 , H. Li 1 , and H. Zhu
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  • 1 Nanjing University of Chinese Medicine College of Pharmacy Nanjing PR China
  • 2 Nanjing University of Chinese Medicine College of Pharmacy 282 Hanzhong Road Nanjing 210029 PR China
DOI:
https://doi.org/10.1556/aphysiol.99.2012.3.8
Page Count:
311–323
Publication Date:
01 Sep 2012
Online Publication Date:
14 Sep 2012
Article Category:
Research Article
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Berberine, a primary pharmacological active constitute of Coptidis Rhizoma, could inhibit neuronal apoptosis in cerebral ischemia. Here, we aimed to investigate whether and how HIF-1 is implicated in the anti-apoptosis effect of berberine on neurons under hypoxia/ischemia. Viability of PC12 cells treated with berberine prior to or following CoCl2-induced hypoxia was evaluated. Annexin V-PI staining was employed to analyse cell apoptosis ratio. HIF-1α and apoptosis-associated molecules were detected via Western blotting. TUNEL and immunohistochemistry were used to demonstrate apoptosis, HIF-1α and p53 levels in cerebral tissue of middle cerebral artery occlusion (MCAO) rats. Berberine pretreatment promoted PC12 cells survival and inhibited apoptosis under hypoxia condition. At the same time, it decreased cell viability and enhancement of apoptosis were observed with berberine treatment under hypoxia. Decreased HIF-1α, caspase 9, caspase 3 and increased Bcl-2/Bax ratio were responsible for the anti-apoptosis of berberine pretreatment. However, pro-apoptosis by berberine under hypoxia was indicated with opposing regulation of those molecules. Significant reduction of apoptosis, HIF-1α and p53 were found in cerebral tissue of MCAO rats treated with berberine. The present study suggests that berberine regulates neuronal apoptosis in cerebral ischemia, which might be dependent on the degree of cell injury. HIF-1 and the followed apoptotic pathway are involved in those effects of berberine.

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Cover Acta Physiologica Hungarica
Acta Physiologica Hungarica
Print ISSN:
0231-424X
Online ISSN:
1588-2683
Keywords:
berberine; anti-apoptosis; pro-apoptosis; cerebral ischemia; HIF-1

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