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  • 1 University of Pécs Department of Pathophysiology and Gerontology Pécs Hungary
  • 2 University of Pécs Szentágothai Research Centre Pécs Hungary
  • 3 University of Pécs Department of Medical Biology Pécs Hungary
  • 4 New York Medical College Department of Physiology Valhalla NYC USA
  • 5 University of Pécs Department of Pathophysiology and Gerontology Szigeti út 12 H-7624 Pécs Hungary
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Recent studies suggest that hydrogen sulfide (H2S) exhibits potent antioxidant capacity and improves vascular and tissue functions. Thus we aimed to compare the antioxidant efficacy of H2S to that of superoxide dismutase (SOD).Isometric force of isolated rat carotid arteries and gracilis veins was measured with a myograph. The vasomotor effect of the superoxide-generator pyrogallol (10−5M) was obtained in control conditions, and then in the presence of SOD (120 U/ml) or H2S (10−5M or 10−4M), respectively. Spectrophotometric measurements were performed to detect the effect of SOD and H2S on the auto-oxidation of pyrogallol.Pyrogallol increased the isometric force of carotid arteries (9.7 ± 0.8 mN), which was abolished by SOD (5.3 ± 0.8 mN), was not affected by 10−5M H2S (9.1 ± 0.5 mN), whereas 10−4M H2S slightly, but significantly reduced it (8.1 ± 0.7 mN). Pyrogallol significantly increased the isometric force of gracilis veins (1.3 ± 0.2 mN), which was abolished by SOD (0.9 ± 0.2 mN), whereas 10−5M (1.3 ± 0.2 mN), or 10−4M H2S (1.2 ± 0.2 mN) did not affect it. Pyrogallol-induced superoxide production was measured by a spectrophotometer (A420 = 0.19 ± 0.0). SOD reduced absorbance (A420 = 0.02 ± 0.0), whereas 10−5M H2S did not (A420 = 0.18 ± 0.0) and 10−4M H2S slightly reduced it (A420 = 0.15 ± 0.0).These data suggest that H2S is a less effective vascular antioxidant than SOD. We propose that the previously described beneficial effects of H2S are unlikely to be related to its direct effect on superoxide.

  • Bagi Z, Koller A: Lack of nitric oxide mediation of flow-dependent arteriolar dilation in type I diabetes is restored by sepiapterin. J. Vasc. Res. 40, 47–57 (2003)

    Koller A. , 'Lack of nitric oxide mediation of flow-dependent arteriolar dilation in type I diabetes is restored by sepiapterin ' (2003 ) 40 J. Vasc. Res. : 47 -57.

    • Search Google Scholar
  • Bagi Z, Erdei N, Koller A: High intraluminal pressure via H2O2 upregulates arteriolar constrictions to angiotensin II by increasing the functional availability of AT1 receptors. Am. J. Physiol. Heart Circ. Physiol. 295, H835–841 (2008)

    Koller A. , 'High intraluminal pressure via H2O2 upregulates arteriolar constrictions to angiotensin II by increasing the functional availability of AT1 receptors ' (2008 ) 295 Am. J. Physiol. Heart Circ. Physiol. : H835 -841.

    • Search Google Scholar
  • Bagi Z, Cseko C, Toth E et al.: Oxidative stress-induced dysregulation of arteriolar wall shear stress and blood pressure in hyperhomocysteinemia is prevented by chronic vitamin C treatment. Am. J. Physiol. Heart Circ. Physiol. 285, H2277–2283 (2003)

    Toth E. , 'Oxidative stress-induced dysregulation of arteriolar wall shear stress and blood pressure in hyperhomocysteinemia is prevented by chronic vitamin C treatment ' (2003 ) 285 Am. J. Physiol. Heart Circ. Physiol. : H2277 -2283.

    • Search Google Scholar
  • Bai XY, Liu XC, Jing WB et al.: Effect of amlodipine in human internal mammary artery and clinical implications. Ann. Thorac. Surg. 90, 1952–1957 (2010)

    Jing W.B. , 'Effect of amlodipine in human internal mammary artery and clinical implications ' (2010 ) 90 Ann. Thorac. Surg. : 1952 -1957.

    • Search Google Scholar
  • Benavides GA, Squadrito GL, Mills RW et al.: Hydrogen sulfide mediates the vasoactivity of garlic. Proc. Natl Acad. Sci. USA 104, 17977–17982 (2007)

    Mills R.W. , 'Hydrogen sulfide mediates the vasoactivity of garlic ' (2007 ) 104 Proc. Natl Acad. Sci. USA : 17977 -17982.

    • Search Google Scholar
  • Elrod JW, Calvert JW, Morrison J et al.: Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function. Proc. Natl Acad. Sci. USA 104, 15560–15565 (2007)

    Morrison J. , 'Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function ' (2007 ) 104 Proc. Natl Acad. Sci. USA : 15560 -15565.

    • Search Google Scholar
  • Evans JL, Goldfine ID, Maddux BA et al.: Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction? Diabetes 52, 1–8 (2003)

    Maddux B.A. , 'Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction? ' (2003 ) 52 Diabetes : 1 -8.

    • Search Google Scholar
  • Griendling KK, FitzGerald GA: Oxidative stress and cardiovascular injury: Part II: animal and human studies. Circulation 108, 2034–2040 (2003)

    FitzGerald G.A. , 'Oxidative stress and cardiovascular injury: Part II: animal and human studies ' (2003 ) 108 Circulation : 2034 -2040.

    • Search Google Scholar
  • Hosoki R, Matsuki N, Kimura H: The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide. Biochem. Biophys. Res. Commun. 237, 527–531 (1997)

    Kimura H. , 'The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide ' (1997 ) 237 Biochem. Biophys. Res. Commun. : 527 -531.

    • Search Google Scholar
  • Ignarro LJ, Byrns RE, Buga GM et al.: Pharmacological evidence that endothelium-derived relaxing factor is nitric oxide: use of pyrogallol and superoxide dismutase to study endothelium-dependent and nitric oxide-elicited vascular smooth muscle relaxation. J. Pharmacol. Exp. Ther. 244, 181–189 (1988)

    Buga G.M. , 'Pharmacological evidence that endothelium-derived relaxing factor is nitric oxide: use of pyrogallol and superoxide dismutase to study endothelium-dependent and nitric oxide-elicited vascular smooth muscle relaxation ' (1988 ) 244 J. Pharmacol. Exp. Ther. : 181 -189.

    • Search Google Scholar
  • Kimura Y, Kimura H: Hydrogen sulfide protects neurons from oxidative stress. FASEB J. 18, 1165–1167 (2004)

    Kimura H. , 'Hydrogen sulfide protects neurons from oxidative stress ' (2004 ) 18 FASEB J. : 1165 -1167.

    • Search Google Scholar
  • Lai EY, Wellstein A, Welch WJ et al.: Superoxide modulates myogenic contractions of mouse afferent arterioles. Hypertension 58, 650–656 (2011)

    Welch W.J. , 'Superoxide modulates myogenic contractions of mouse afferent arterioles ' (2011 ) 58 Hypertension : 650 -656.

    • Search Google Scholar
  • Loschen G, Azzi A, Richter C et al.: Superoxide radicals as precursors of mitochondrial hydrogen peroxide. FEBS Lett. 42, 68–72 (1974)

    Richter C. , 'Superoxide radicals as precursors of mitochondrial hydrogen peroxide ' (1974 ) 42 FEBS Lett. : 68 -72.

    • Search Google Scholar
  • Machado FR, Ferreira AGK, da Cunha AA et al.: Homocysteine alters glutamate uptake and Na(+), K(+)-ATPase activity and oxidative status in rats hippocampus: protection by vitamin C. Metabolic Brain Disease 26, 61–67 (2011)

    Cunha A.A. , 'Homocysteine alters glutamate uptake and Na(+), K(+)-ATPase activity and oxidative status in rats hippocampus: protection by vitamin C ' (2011 ) 26 Metabolic Brain Disease : 61 -67.

    • Search Google Scholar
  • Marklund S, Marklund G: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469–474 (1974)

    Marklund G. , 'Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase ' (1974 ) 47 Eur. J. Biochem. : 469 -474.

    • Search Google Scholar
  • Martelli A, Testai L, Breschi MC et al.: Hydrogen sulphide: novel opportunity for drug discovery. Med. Res. Rev., DOI: 10.1002/med.20234 (2010)

  • Mitsuhashi H, Yamashita S, Ikeuchi H et al.: Oxidative stress-dependent conversion of hydrogen sulfide to sulfite by activated neutrophils. Shock 24, 529–534 (2005)

    Ikeuchi H. , 'Oxidative stress-dependent conversion of hydrogen sulfide to sulfite by activated neutrophils ' (2005 ) 24 Shock : 529 -534.

    • Search Google Scholar
  • Mulvany MJ, Halpern W: Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ. Res. 41, 19–26 (1977)

    Halpern W. , 'Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats ' (1977 ) 41 Circ. Res. : 19 -26.

    • Search Google Scholar
  • Muzaffar S, Shukla N, Bond M et al.: Exogenous hydrogen sulfide inhibits superoxide formation, NOX-1 expression and Rac1 activity in human vascular smooth muscle cells. J. Vasc. Res. 45, 521–528 (2008)

    Bond M. , 'Exogenous hydrogen sulfide inhibits superoxide formation, NOX-1 expression and Rac1 activity in human vascular smooth muscle cells ' (2008 ) 45 J. Vasc. Res. : 521 -528.

    • Search Google Scholar
  • Ou HC, Lee WJ, Wu CM et al.: Aspirin prevents resistin-induced endothelial dysfunction by modulating AMPK, ROS, and Akt/eNOS signaling. J. Vasc. Surg. 55, 1104–1115 (2012)

    Wu C.M. , 'Aspirin prevents resistin-induced endothelial dysfunction by modulating AMPK, ROS, and Akt/eNOS signaling ' (2012 ) 55 J. Vasc. Surg. : 1104 -1115.

    • Search Google Scholar
  • Pourcyrous M, Busija DW, Shibata M et al.: Cerebrovascular responses to therapeutic dose of indomethacin in newborn pigs. Pediatr. Res. 45, 582–587 (1999)

    Shibata M. , 'Cerebrovascular responses to therapeutic dose of indomethacin in newborn pigs ' (1999 ) 45 Pediatr. Res. : 582 -587.

    • Search Google Scholar
  • Prieto D, Kaminski PM, Bagi Z et al.: Hypoxic relaxation of penile arteries: involvement of endothelial nitric oxide and modulation by reactive oxygen species. Am. J. Physiol. Heart Circ. Physiol. 299, H915–924 (2010)

    Bagi Z. , 'Hypoxic relaxation of penile arteries: involvement of endothelial nitric oxide and modulation by reactive oxygen species ' (2010 ) 299 Am. J. Physiol. Heart Circ. Physiol. : H915 -924.

    • Search Google Scholar
  • Rajchel L, Rajchel J, Szaran J et al.: Sulfur isotopic composition of H2S and SO4(2−) from mineral springs in the Polish Carpathians. Isotopes Environ. Health Stud. 38, 277–284 (2002)

    Szaran J. , 'Sulfur isotopic composition of H2S and SO4(2−) from mineral springs in the Polish Carpathians ' (2002 ) 38 Isotopes Environ. Health Stud. : 277 -284.

    • Search Google Scholar
  • Sodha NR, Clements RT, Feng J et al.: Hydrogen sulfide therapy attenuates the inflammatory response in a porcine model of myocardial ischemia/reperfusion injury. J. Thorac. Cardiovasc. Surg. 138, 977–984 (2009)

    Feng J. , 'Hydrogen sulfide therapy attenuates the inflammatory response in a porcine model of myocardial ischemia/reperfusion injury ' (2009 ) 138 J. Thorac. Cardiovasc. Surg. : 977 -984.

    • Search Google Scholar
  • Szabo C: Hydrogen sulphide and its therapeutic potential. Nat. Rev. Drug Discov. 6, 917–935 (2007)

    Szabo C. , 'Hydrogen sulphide and its therapeutic potential ' (2007 ) 6 Nat. Rev. Drug Discov. : 917 -935.

    • Search Google Scholar
  • Taniyama Y, Griendling KK: Reactive oxygen species in the vasculature: molecular and cellular mechanisms. Hypertension 42, 1075–1081 (2003)

    Griendling K.K. , 'Reactive oxygen species in the vasculature: molecular and cellular mechanisms ' (2003 ) 42 Hypertension : 1075 -1081.

    • Search Google Scholar
  • Tomaskova Z, Bertova A, Ondrias K: On the involvement of H2S in nitroso signaling and other mechanisms of H2S action. Curr. Pharm. Biotechnol. 12, 1394–1405 (2011)

    Ondrias K. , 'On the involvement of H2S in nitroso signaling and other mechanisms of H2S action ' (2011 ) 12 Curr. Pharm. Biotechnol. : 1394 -1405.

    • Search Google Scholar
  • Toth J, Racz A, Kaminski PM et al.: Asymmetrical dimethylarginine inhibits shear stress-induced nitric oxide release and dilation and elicits superoxide-mediated increase in arteriolar tone. Hypertension 49, 563–568 (2007)

    Kaminski P.M. , 'Asymmetrical dimethylarginine inhibits shear stress-induced nitric oxide release and dilation and elicits superoxide-mediated increase in arteriolar tone ' (2007 ) 49 Hypertension : 563 -568.

    • Search Google Scholar
  • Tsutsui H, Kinugawa S, Matsushima S: Oxidative stress and heart failure. Am. J. Physiol. Heart Circ. Physiol. 301, H2181–2190 (2011)

    Matsushima S. , 'Oxidative stress and heart failure ' (2011 ) 301 Am. J. Physiol. Heart Circ. Physiol. : H2181 -2190.

    • Search Google Scholar
  • Turrens JF: Mitochondrial formation of reactive oxygen species. J. Physiol. 552, 335–344 (2003)

    Turrens J.F. , 'Mitochondrial formation of reactive oxygen species ' (2003 ) 552 J. Physiol. : 335 -344.

    • Search Google Scholar
  • Tyml K: Critical role for oxidative stress, platelets, and coagulation in capillary blood flow impairment in sepsis. Microcirculation 18, 152–162 (2011)

    Tyml K. , 'Critical role for oxidative stress, platelets, and coagulation in capillary blood flow impairment in sepsis ' (2011 ) 18 Microcirculation : 152 -162.

    • Search Google Scholar
  • Vaidya AD, Vaidya R: Reactive oxygen species, anti-oxidant enzymes and smoldering chronic inflammation: Relevance to diabetes mellitus, atherosclerosis, and menopausal metabolic syndrome. J. Midlife Health 2, 49–50 (2011)

    Vaidya R. , 'Reactive oxygen species, anti-oxidant enzymes and smoldering chronic inflammation: Relevance to diabetes mellitus, atherosclerosis, and menopausal metabolic syndrome ' (2011 ) 2 J. Midlife Health : 49 -50.

    • Search Google Scholar
  • Whiteman M, Cheung NS, Zhu YZ et al.: Hydrogen sulphide: a novel inhibitor of hypochlorous acid-mediated oxidative damage in the brain? Biochem. Biophys. Res. Commun. 326, 794–798 (2005)

    Zhu Y.Z. , 'Hydrogen sulphide: a novel inhibitor of hypochlorous acid-mediated oxidative damage in the brain? ' (2005 ) 326 Biochem. Biophys. Res. Commun. : 794 -798.

    • Search Google Scholar
  • Yan H, Du J, Tang C: The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats. Biochem. Biophys. Res. Commun. 313, 22–27 (2004)

    Tang C. , 'The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats ' (2004 ) 313 Biochem. Biophys. Res. Commun. : 22 -27.

    • Search Google Scholar
  • Yan SK, Chang T, Wang H et al.: Effects of hydrogen sulfide on homocysteine-induced oxidative stress in vascular smooth muscle cells. Biochem. Biophys. Res. Commun. 351, 485–491 (2006)

    Wang H. , 'Effects of hydrogen sulfide on homocysteine-induced oxidative stress in vascular smooth muscle cells ' (2006 ) 351 Biochem. Biophys. Res. Commun. : 485 -491.

    • Search Google Scholar

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