Petri Dish-ELISA, a Simple and Economic Technique for Detecting Plant Viruses

M. E. Abdalla; S. E. Albrechtsen

doi:10.1556/aphyt.36.2001.1-2.1

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Acta Phytopathologica et Entomologica Hungarica

Volume/Issue:: Volume 36: Issue 1-2

Petri Dish-ELISA, a Simple and Economic Technique for Detecting Plant Viruses

Authors:

M. E. Abdalla

M. E. Abdalla Please ask the editor of the journal.

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S. E. Albrechtsen

S. E. Albrechtsen
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Pages:: 1–8

Online Publication Date:: 22 Jul 2005

Publication Date:: 01 Jun 2001

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/aphyt.36.2001.1-2.1

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A large number of microtiter plates are needed for mass testing of planting material for viruses in seed certification and plant quarantine. In the case of poorly equipped laboratories, problems with availability of microtiter plates have economic implications for the broad acceptance of enzyme-linked immunosorbent assay (ELISA) for e.g. seed health testing in developing countries. In this experiment the potential of an alternative, cheaper technique was investigated. A conventional indirect ELISA procedure was followed for comparison between the polystyrene solid phases of plastic Petri dishes and microtiter plates for detection of three viruses belonging to the Tobamovirus, Comovirus and Potyvirus genera. A wax pen was used to divide the inner surface of a polystyrene Petri dish into many circles or squares.

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Acta Phytopathologica et Entomologica Hungarica

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Jenő KONTSCHÁN Centre for Agricultural Research, Hungary

Technical Editor

Ágnes TURÓCI Centre for Agricultural Research, Hungary

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K SALÁNKI Centre for Agricultural Research, Hungary

Editorial Board

Z BOZSÓ Centre for Agricultural Research, Hungary

PE CHETVERIKOV Saint-Petersburg State University, Russia

JX CUI Henan Institute of Science and Technology, China

J FODOR Centre for Agricultural Research, Hungary

Z IMREI Centre for Agricultural Research, Hungary

BM KAYDAN Çukurova University, Turkey

L KISS University of Southern Queensland, Australia

V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary

MW NEGM Ibaraki University, Japan

L PALKOVICS Széchenyi István University, Hungary

M POGÁNY Centre for Agricultural Research, Hungary

D RÉDEI National Chung Hsing University, Taiwan

A TOLSTIKOV University of Tyumen, Russia

J VUTS Rothamsted Research, UK

GQ WANG Guangxi University, China

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Acta Phytopathologica et Entomologica Hungarica
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