Acta Phytopathologica et Entomologica Hungarica
Volume/Issue:
Volume 38: Issue 1-2
Novel Genotypes in Phytophthora infestans Populations in Hungary
Authors: Z. Á. Nagy 1 , J. Bakonyi 2 , and T. Érsek 3
View More View Less
  • 1 Plant Protection Institute, Hungarian Academy of Sciences, H-1525 Budapest, P.O. Box 102, Hungary
  • 2 Plant Protection Institute, Hungarian Academy of Sciences, H-1525 Budapest, P.O. Box 102, Hungary
  • 3 Plant Protection Institute, Hungarian Academy of Sciences, H-1525 Budapest, P.O. Box 102, Hungary
DOI:
https://doi.org/10.1556/aphyt.38.2003.1-2.2
Page Count:
7–11
Publication Date:
01 Apr 2003
Online Publication Date:
22 Jul 2005
Article Category:
Research Article
Restricted access

Purchase article

USD  $20.00

1 year subscription (Individual Only)

USD  $542.00
Purchase Online Subscription

  • Purchase article

    USD  $20.00

  • 1 year subscription (Individual Only)

    USD  $542.00

Twenty-seven isolates of Phytophthora infestans collected in Hungary in 2001 were tested for mating type, response to metalaxyl, isozyme geno­type at glucose-6-phosphate isomerase (Gpi) and peptidase A (Pep) loci and nuclear DNA fingerprints with probe RG57. The ratios of the mating types A1 to A2 were 5:6 and 9:7 among isolates from potato and tomato, respectively. Seventeen isolates were sensitive to metalaxyl, 1 isolate responded intermediately and 9 isolates were resistant. No novel combi­nations of isozyme alleles were found; all isolates were Gpi 100/100, and genotypes at the Pep locus were 96/96 (63%), 83/96 (11%) and 100/100 (26%). In contrast, all of the 22 RG57 fingerprints exhibited patterns that have not been reported in Hungary before. On the basis of combined traits, 22 multilocus genotypes, unnoted elsewhere in Europe, were con­struct­ed among the 27 isolates analysed. These results indicate that varia­bi­lity in the Hungarian P. infestans populations is likely due to local events (asexual and sexual interactions) rather than migration from other countries.

  • Anonym (1995): The DIG System Users' Guide for Filter Hybridization. Boehringer Mannheim GmbH Biochemica.

  • Bakonyi, J., Láday, M., Dula, T. and Érsek, T. (1998): Characterization of Phytophthora infestans isolates from Hungary. Acta Phytopathol. Entomol. Hung. 33, 49-54.

    'Characterization of Phytophthora infestans isolates from Hungary ' () 33 Acta Phytopathol. Entomol. Hung. : 49 -54.

    • Search Google Scholar

  • Bakonyi, J., Láday, M., Dula, T. and Érsek, T. (2002): Characterisation of isolates of Phytophthora infestans from Hungary. Eur. J. Plant Pathol. 108, 139-146.

    'Characterisation of isolates of Phytophthora infestans from Hungary ' () 108 Eur. J. Plant Pathol. : 139 -146.

    • Search Google Scholar

  • Drenth, A., Goodwin, S. B., Fry, W. E. and Davidse, L. C. (1993): Genotypic diversity of Phytophthora infestans in The Netherlands revealed by DNA polymorphisms. Phytopathology 83, 1087-1092.

    'Genotypic diversity of Phytophthora infestans in The Netherlands revealed by DNA polymorphisms ' () 83 Phytopathology : 1087 -1092.

    • Search Google Scholar

  • Érsek, T. and Bakonyi, J. (1997): Potato late blight: the disease and the pathogen. Növényvédelem (Plant Protection) 33, 353-382. (In Hungarian, English summary)

    'Potato late blight: the disease and the pathogen ' () 33 Növényvédelem (Plant Protection) : 353 -382.

    • Search Google Scholar

  • Fry, W. E., Goodwin, S. B., Dyer, A. T., Matuszak, J. M., Drenth, A., Tooley, P. W., Sujkowski, L. S., Koh, Y. J., Cohen, B. A., Spielman, L. J., Deahl, K. L., Inglis, D. A. and Sandlan, K. P. (1993): Historical and recent migrations of Phytophthora infestans: chronology, pathways, and implications. Plant Dis. 77, 653-661.

    'Historical and recent migrations of Phytophthora infestans: chronology, pathways, and implications ' () 77 Plant Dis. : 653 -661.

    • Search Google Scholar

  • Forbes, G. A., Goodwin, S. B., Drenth, A., Oyarzun, P., Ordoñez, M. E. and Fry, W. E. (1998): A global marker database for Phytophthora infestans. Plant Dis. 82, 811-818.

    'A global marker database for Phytophthora infestans ' () 82 Plant Dis. : 811 -818.

  • Fry, W. E. and Goodwin, S. B. (1995): Recent migrations of Phytophthora infestans. In: Dowley, L. J., Bannon, E., Cooke, L. R., Keane, T. and O'Sullivan, E. (eds) Phytophthora infestans 150. Boole Press Ltd. Dublin, Ireland. pp. 89-95.

    Phytophthora infestans , () 89 -95.

  • Gavino, P. D., Smart, C. D., Sandrock, R. W., Miller, J. S., Hamm, P. B., Lee, T. Y., Davis, R. M. and Fry, W. E. (2000): Implication of sexual reproduction for Phytophthora infestans in the United States: generation of an aggressive lineage. Plant Dis. 84, 731-735.

    'Implication of sexual reproduction for Phytophthora infestans in the United States: generation of an aggressive lineage ' () 84 Plant Dis. : 731 -735.

    • Search Google Scholar

  • Goodwin, S. B., Drenth, A. and Fry, W. E. (1992): Cloning and genetic analyses of two highly polymorphic, moderately repetitive nuclear DNAs from Phytophthora infestans. Curr. Genet. 22, 107-115.

    'Cloning and genetic analyses of two highly polymorphic, moderately repetitive nuclear DNAs from Phytophthora infestans ' () 22 Curr. Genet. : 107 -115.

    • Search Google Scholar

  • Goodwin, S. B., Schneider, R. E. and Fry, W. E. (1995): Use of Cellulose-Acetate Electrophoresis for Rapid Identification of Allozyme Genotypes of Phytophthora infestans. Plant Dis. 79, 1181-1185.

    'Use of Cellulose-Acetate Electrophoresis for Rapid Identification of Allozyme Genotypes of Phytophthora infestans ' () 79 Plant Dis. : 1181 -1185.

    • Search Google Scholar

  • Hebert, P. D. N. and Beaton, M. J. (1993): Methodologies for Allozyme Analysis Using Cellulose Acetate Electrophoresis. A Practical Handbook. Helena Laboratories, Beaumont, TX.

    Methodologies for Allozyme Analysis Using Cellulose Acetate Electrophoresis. A Practical Handbook , ().

    • Search Google Scholar

  • Hohl, H. R. and Iselin, K. (1984): Strains of Phytophthora infestans with A2 mating type behaviour. Trans. Br. Mycol. Soc. 83, 529-530.

    'Strains of Phytophthora infestans with A2 mating type behaviour ' () 83 Trans. Br. Mycol. Soc. : 529 -530.

    • Search Google Scholar

  • Koh, Y. J., Goodwin, S. B., Dyer, A. T., Cohen, B. A., Ogoshi, A., Sato, N. and Fry, W. E. (1994): Migration and displacements of Phytophthora infestans in East Asian Countries. Phytopathology 84, 922-927.

    'Migration and displacements of Phytophthora infestans in East Asian Countries ' () 84 Phytopathology : 922 -927.

    • Search Google Scholar

  • Láday, M., Bagi, F., Mesterházy, Á. and Szécsi, Á. (2000): Isozyme evidence for two groups of Fusarium graminearum. Mycol. Res. 104, 788-793.

    'Isozyme evidence for two groups of Fusarium graminearum ' () 104 Mycol. Res. : 788 -793.

  • Mudich, A. (1965): Biotypes of Phytophthora infestans in southern Transdanubia, Hungary. Növényvédelem (Plant Protection) 1, 27-32. (In Hungarian)

    'Biotypes of Phytophthora infestans in southern Transdanubia, Hungary ' () 1 Növényvédelem (Plant Protection) : 27 -32.

    • Search Google Scholar

  • Sujkowski, L. S., Goodwin, S. B., Dyer, A. T. and Fry, W. E. (1994): Increased genotypic diversity via migration and possible occurrence of sexual reproduction of Phytophthora infestans in Poland. Phytopathology 84, 201-207.

    'Increased genotypic diversity via migration and possible occurrence of sexual reproduction of Phytophthora infestans in Poland ' () 84 Phytopathology : 201 -207.

    • Search Google Scholar

  • Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989): Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

    Molecular Cloning: A Laboratory Manual , ().

  • Spielman, L. J., Drenth, A., Davidse, L. C., Sujkowski, L. S., Gu, W. K., Tooley, P. W. and Fry, W. E. (1991): A second world-wide migration and population displacement of Phytophthora infestans? Plant Pathol. 40, 422-430.

    'A second world-wide migration and population displacement of Phytophthora infestans? ' () 40 Plant Pathol. : 422 -430.

    • Search Google Scholar
Cover Acta Phytopathologica et Entomologica Hungarica
Acta Phytopathologica et Entomologica Hungarica
Print ISSN:
0238-1249
Online ISSN:
1588-2691
Keywords:
Isozymes; Isozymes; mating type; metalaxyl sensitivity; RFLP-DNA fingerprints; multilocus genotypes; potato late blight.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 1 0 0
Sep 2020 0 0 0
Oct 2020 0 0 0
Nov 2020 0 0 0
Dec 2020 3 0 0
Jan 2021 0 0 0
Feb 2021 0 0 0