In vitro Antifungal Activity of a 29-kDa Glycoprotein Purified from the Galls of Quercus infectoria

K. Yamunarani; R. Jaganathan; R. Bhaskaran; P. Govindaraju; R. Velazhahan

doi:10.1556/aphyt.40.2005.1-2.6

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Acta Phytopathologica et Entomologica Hungarica

Volume/Issue:: Volume 40: Issue 1-2

In vitro Antifungal Activity of a 29-kDa Glycoprotein Purified from the Galls of Quercus infectoria

Authors:

K. Yamunarani

K. Yamunarani Department of Plant Pathology, Tamil Nadu Agricultural University Madurai-625 104, Tamil Nadu, India

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R. Jaganathan

R. Jaganathan Department of Plant Pathology, Tamil Nadu Agricultural University Madurai-625 104, Tamil Nadu, India

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R. Bhaskaran

R. Bhaskaran Department of Plant Pathology, Tamil Nadu Agricultural University Madurai-625 104, Tamil Nadu, India

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P. Govindaraju

P. Govindaraju Department of Physical Sciences, Agricultural College and Research Institute, Tamil Nadu Agricultural University Madurai-625 104, Tamil Nadu, India

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, and

R. Velazhahan

R. Velazhahan Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore-641 003, Tamil Nadu, India

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Pages:: 43–54

Online Publication Date:: 22 Jul 2005

Publication Date:: 01 Apr 2005

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/aphyt.40.2005.1-2.6

Keywords:: antifungal protein; antifungal protein; Quercus infectoria; protein purification.

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The antifungal activity of 30 medicinal plants belonging to different families was tested in vitro on the phytopathogenic fungus Rhizoctonia solani. The results revealed that protein extract from the galls of Quercus infectoria belonging to Fagaceae family was highly effective in inhibiting the mycelial growth of R. solani. The gall extract of Q. infectoria also inhibited the growth of other agronomically important fungal pathogens viz. Fusarium oxysporum, Cochliobolus miyabeanus, Macrophomina phaseolina, Colletotrichum gloeosporioides, Magnaporthe salvinii, Cochliobolus lunatus, Alternaria solani, Pythium aphanidermatum and Colletotrichum falcatum. A 29-kDa glycoprotein was purified from the galls of Q. infectoria by ammonium sulphate fractionation followed by gel filtration on Sephadex G-50 column. The purified protein showed the absorption maxima at 640 nm and 308 nm. The purified protein was stable even after heating at 100 °C for 10 min or autoclaving at 121 °C for 20 min. When the 29-kDa protein was treated with NaIO4 and pronase the antifungal activity was drastically reduced. The 29-kDa protein inhibited the mycelial growth of R. solani and C. miyabeanus at 2 µg level.

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Acta Phytopathologica et Entomologica Hungarica

Print ISSN:: 0238-1249

Online ISSN:: 1588-2691

Editor-in-Chief

Jenő KONTSCHÁN Centre for Agricultural Research, Hungary

Technical Editor

Ágnes TURÓCI Centre for Agricultural Research, Hungary

Section Editor

K SALÁNKI Centre for Agricultural Research, Hungary

Editorial Board

Z BOZSÓ Centre for Agricultural Research, Hungary

PE CHETVERIKOV Saint-Petersburg State University, Russia

JX CUI Henan Institute of Science and Technology, China

J FODOR Centre for Agricultural Research, Hungary

Z IMREI Centre for Agricultural Research, Hungary

BM KAYDAN Çukurova University, Turkey

L KISS University of Southern Queensland, Australia

V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary

MW NEGM Ibaraki University, Japan

L PALKOVICS Széchenyi István University, Hungary

M POGÁNY Centre for Agricultural Research, Hungary

D RÉDEI National Chung Hsing University, Taiwan

A TOLSTIKOV University of Tyumen, Russia

J VUTS Rothamsted Research, UK

GQ WANG Guangxi University, China

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Acta Phytopathologica et Entomologica Hungarica
Language	English
Size	B5
Year of Foundation	1966
Volumes per Year	1
Issues per Year	2
Founder	Magyar Tudományos Akadémia
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