Molecular detection of Cucumber mosaic virus in Chrysanthemum cultivars

S. Kumar; A. Srivastava; S. K. Raj

doi:10.1556/aphyt.40.2005.3-4.2

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Acta Phytopathologica et Entomologica Hungarica

Volume/Issue:: Volume 40: Issue 3-4

Molecular detection of Cucumber mosaic virus in Chrysanthemum cultivars

Authors:

S. Kumar

S. Kumar Molecular Virology, National Botanical Research Institute, Lucknow-226001, India

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A. Srivastava

A. Srivastava Molecular Virology, National Botanical Research Institute, Lucknow-226001, India

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S. K. Raj

S. K. Raj Molecular Virology, National Botanical Research Institute, Lucknow-226001, India

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Pages:: 205–211

Online Publication Date:: 18 Jan 2006

Publication Date:: 01 Dec 2005

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/aphyt.40.2005.3-4.2

Keywords:: molecular detection of Cucumber mosaic virus; molecular detection of Cucumber mosaic virus

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Molecular detection of Cucumber mosaic virus in various chrysanthemum cultivars was attempted by RT-PCR and Southern hybridization tests using CMV specific primers and cloned probes, respectively. A pair of primers was designed from a conserved region of the Cucumber mosaic virus coat protein (CMV-CP) gene, capable of amplifying a product of ~650 bp from various CMV strains. RT-PCR using the total nucleic acid from infected leaf samples and the specific primers resulted in positive amplification of an expected size band of ~650 bp in most of the samples. The identity of the PCR amplicons was checked by Southern hybridization using the a32P-labelled DNA probes prepared from the cloned coat protein gene of a well-identified strain of CMV isolated from Amaranthus. Positive signal of hybridization of PCR products and CMV probes confirmed the identity of fected chrysanthemum samples.

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Acta Phytopathologica et Entomologica Hungarica

Print ISSN:: 0238-1249

Online ISSN:: 1588-2691

Editor-in-Chief

Jenő KONTSCHÁN Centre for Agricultural Research, Hungary

Technical Editor

Ágnes TURÓCI Centre for Agricultural Research, Hungary

Section Editor

K SALÁNKI Centre for Agricultural Research, Hungary

Editorial Board

Z BOZSÓ Centre for Agricultural Research, Hungary

PE CHETVERIKOV Saint-Petersburg State University, Russia

JX CUI Henan Institute of Science and Technology, China

J FODOR Centre for Agricultural Research, Hungary

Z IMREI Centre for Agricultural Research, Hungary

BM KAYDAN Çukurova University, Turkey

L KISS University of Southern Queensland, Australia

V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary

MW NEGM Ibaraki University, Japan

L PALKOVICS Széchenyi István University, Hungary

M POGÁNY Centre for Agricultural Research, Hungary

D RÉDEI National Chung Hsing University, Taiwan

A TOLSTIKOV University of Tyumen, Russia

J VUTS Rothamsted Research, UK

GQ WANG Guangxi University, China

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Acta Phytopathologica et Entomologica Hungarica
Language	English
Size	B5
Year of Foundation	1966
Volumes per Year	1
Issues per Year	2
Founder	Magyar Tudományos Akadémia
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ISSN	1588-2691 (Online)