View More View Less
  • 1 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore-641003, India
  • 2 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore-641003, India
  • 3 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore-641003, India
  • 4 Senior Scientist, Indian Institute of Horticultural Research, Hassagutta, Bangalore, India
  • 5 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore-641003, India
  • 6 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore-641003, India
  • 7 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore-641003, India
Restricted access

Purchase article

USD  $20.00

1 year subscription (Individual Only)

USD  $542.00

A new necrosis viral disease was observed in blackgram, showed brown necrotic rings along with veinal and stem necrosis. The virus was mechanically inoculated on the local lesion host, cowpea cv. 152 and maintained in the local lesion host throughout the study. Yield studies under pot culture experiment showed 10- to 30-day-old plants were highly susceptible and the yield became almost nil. By using Transmission Electron Microscope (TEM) and indirect Direct Antigen Coated-Enzyme Linked Immuno-Sorbent Assay (DAC-ELISA) studies the virus was identified as Tobacco streak virus (TSV). The ultraviolet absorbance of the purified virus was measured and the ratio of A260/A280 was determined as 1.41. Polyclonal antiserum was raised against blackgram necrosis virus in New Zealand white rabbit and the titre value was determined as 1: 200. Direct antigen coating-ELISA was used to detect the virus concentration in various plant parts and stem portion recorded maximum virus concentration. TSV in blackgram was not transmitted through seeds.

  • Gomez, K. A. and Gomez, A. A. (1984): Statistical Procedure for Agricultural Research. John Wiley and Sons, New York.

    Statistical Procedure for Agricultural Research , ().

  • Bhat, A. I., Kumar, Anil Jain, R. K., Chander Rao, S. and Ramiah, M. (2001): Development of serological based assays for the diagnosis of sunflower necrosis disease. Ann. Plant Protect. Sci. 9, 292-296.

    'Development of serological based assays for the diagnosis of sunflower necrosis disease. ' () 9 Ann. Plant Protect. Sci. : 292 -296.

    • Search Google Scholar
  • Cook, G., Le Miranda, J. R., Roossinck, M. J. and Pietersen, G. (1999): Tobacco streak ilarvirus detected on groundnut in South Africa. African Plant Prot. 5, 13-19.

    'Tobacco streak ilarvirus detected on groundnut in South Africa. ' () 5 African Plant Prot. : 13 -19.

    • Search Google Scholar
  • Converse, R. H. (1972): Tobacco streak virus in Black Raspberry. Phytopathology, 62; 1001-1004.

    'Tobacco streak virus in Black Raspberry ' () 62 Phytopathology : 1001 -1004.

  • Cupertino, F. O., Grogan, R. G., Petersen, L. J. and Kimble, K. A. (1984): Tobacco streak virus infection of tomato and some natural weed hosts in California. Plant Dis. 68, 331-333.

    'Tobacco streak virus infection of tomato and some natural weed hosts in California. ' () 68 Plant Dis. : 331 -333.

    • Search Google Scholar
  • Fagbenle, H. H. and Ford, E. E. (1970): Tobacco streak virus isolated from soybeans, Glycine max. Phytopathology 68, 814-820.

    'Tobacco streak virus isolated from soybeans, Glycine max. ' () 68 Phytopathology : 814 -820.

    • Search Google Scholar
  • Kaiser, W. J., Wyatt, S. D. and Pesho, G. R. (1982): Natural hosts and vectors of tobacco streak virus in Eastern Washington. Phytopathology 72, 1508-1512.

    'Natural hosts and vectors of tobacco streak virus in Eastern Washington. ' () 72 Phytopathology : 1508 -1512.

    • Search Google Scholar
  • Narayan Rishi. (1987): Seed and crop improvement of northern India Pulses (Pisum and Vigna) through control of seed borne (mosaic) viruses. Annual Report Haryana Agricultural University, Hissar (Haryana).

  • Patino, G. and Zaumeyer, W. J. (1959). A new strain of tobacco streak virus from peas. Phytopathology 49,43-49.

    'A new strain of tobacco streak virus from peas. ' () 49 Phytopathology : 43 -49.

  • Ramiah, M., Bhat, A. I., Jain, R. K., Pant, R. P., Ahlawat, Y. S., Prabhakar, K. and Varma, A. P. (2001): Partial characterization of an isometric virus causing sunflower necrosis disease. Indian Phytopath. 54, 246-250.

    'Partial characterization of an isometric virus causing sunflower necrosis disease. ' () 54 Indian Phytopath. : 246 -250.

    • Search Google Scholar
  • Reddy, A. S., Prasada Rao, R. D. V. J., Thirumala-Devi, K., Reddy, S. N., Mayo, M. A., Roberts, I., Satyanarayana, T., Subramaniam, K. and Reddy, D. V. R. (2002): Occurrence of Tobacco streak virus on peanut (Arachis hypogaea) in India. Plant Dis. 86, 173-178.

    'Occurrence of Tobacco streak virus on peanut (Arachis hypogaea) in India. ' () 86 Plant Dis. : 173 -178.

    • Search Google Scholar
  • Salazar, L. F., Abad, J. A. and Hooker, W. J. (1981): Host range and properties of a strain of tobacco streak virus from potatoes. Phytopathology, 72, 1550-1554.

    'Host range and properties of a strain of tobacco streak virus from potatoes. ' () 72 Phytopathology : 1550 -1554.

    • Search Google Scholar
  • Spiegel, S., Martin, R. R., Leggett, F., ter Borg M. and Postman, J. (1993): Characterization and geographical distribution of a new ilarvirus from Frageria chiloensis. Phytopathology, 83, 991-995.

    'Characterization and geographical distribution of a new ilarvirus from Frageria chiloensis. ' () 83 Phytopathology : 991 -995.

    • Search Google Scholar
  • Van Dun, C. M. P., Overduin, B., Van Volten-Doting, L. and Bol, J. F. (1988): Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection. Virology 164, 383-389.

    'Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection. ' () 164 Virology : 383 -389.

    • Search Google Scholar
  • Walter, M. H., Kaiser, W. J., Klein, R. F. and Wyatt, S. D. (1992): Association between tobacco streak ilar virus seed transmission and issue infection in bean. Phytopathology 82, 412-415.

    'Association between tobacco streak ilar virus seed transmission and issue infection in bean. ' () 82 Phytopathology : 412 -415.

    • Search Google Scholar
  • Bos, L., Huthinga, H. and Maat, D. Z. (1980): Spinach latent virus, a new ilarvirus seed-borne in Spinacia oleraceae. Ner. J. PI. Path. 86, 79-98.

    'Spinach latent virus, a new ilarvirus seed-borne in Spinacia oleraceae. ' () 86 Ner. J. PI. Path. : 79 -98.

    • Search Google Scholar
  • Bradford, M. M. (1976): A rapid and sensitive method for the quantification of microgram of porteins using the principles of protein-dye binding. Anal. Biochem. 72, 248-254.

    'A rapid and sensitive method for the quantification of microgram of porteins using the principles of protein-dye binding. ' () 72 Anal. Biochem. : 248 -254.

    • Search Google Scholar
  • Gracia, O. and Feldman, J. M. (1974): Tobacco streak virus in pepper. Phytopath. Z. 80, 313-323.

    'Tobacco streak virus in pepper. ' () 80 Phytopath. Z. : 313 -323.

  • Johnson, J. (1936): Tobacco streak, a virus disease. Phytopathology 26,285-292.

    'Tobacco streak, a virus disease. ' () 26 Phytopathology : 285 -292.