Authors:
O. Viczián Department of Biotechnology, Plant Protection Institute, Hungarian Academy of Sciences, H-1525 Budapest, P.O. Box 102, Hungary

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E. Mergenthaler Department of Biotechnology, Plant Protection Institute, Hungarian Academy of Sciences, H-1525 Budapest, P.O. Box 102, Hungary

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S. Süle Department of Biotechnology, Plant Protection Institute, Hungarian Academy of Sciences, H-1525 Budapest, P.O. Box 102, Hungary

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The tuf gene of “Candidatus Phytoplasma mali”, the causal agent of apple proliferation was PCR cloned in an expression vector and expressed in Escherichia coli. First, phytoplasma DNA extracted from periwinkle was amplified using primers designed on the basis of the tuf gene and the PCR product was cloned into pGEM-T (Promega). In the next step specific primers were constructed containing some plasmid sequences and restriction enzyme sites. With this primers the sequence in pGEM-T was amplified, the product was digested with restriction enzymes, and inserted into the pQE40 expression vector (Qiagen). In this plasmid the tuf gene was fused to the 6xHIS tag, and DHFR. The production of 6xHIS-DHFR-Tu fusion protein protein was induced with IPTG and expressed in E. coli M15. The new fusion protein was found in the insoluble fraction of the bacterium. The identity of the protein was verified with polyacrylamid gel-electrophoresis and Western blot analysis using antiserum raised against the 6xHIStag of the fusion protein.

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Editor-in-Chief

Jenő KONTSCHÁN Centre for Agricultural Research, Hungary

Technical Editor

Ágnes TURÓCI Centre for Agricultural Research, Hungary

Section Editor

K SALÁNKI Centre for Agricultural Research, Hungary
 

Editorial Board

Z BOZSÓ Centre for Agricultural Research, Hungary
PE CHETVERIKOV Saint-Petersburg State University, Russia
JX CUI Henan Institute of Science and Technology, China
J FODOR Centre for Agricultural Research, Hungary
Z IMREI Centre for Agricultural Research, Hungary
BM KAYDAN Çukurova University, Turkey
L KISS University of Southern Queensland, Australia
V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary
MW NEGM Ibaraki University, Japan
L PALKOVICS Széchenyi István University, Hungary
M POGÁNY Centre for Agricultural Research, Hungary
D RÉDEI National Chung Hsing University, Taiwan
A TOLSTIKOV University of Tyumen, Russia
J VUTS Rothamsted Research, UK
GQ WANG Guangxi University, China

Acta Phytopathologica et Entomologica Hungarica
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2023  
Scopus  
CiteScore 1.1
CiteScore rank Q4 (Insect Science)
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Acta Phytopathologica et Entomologica Hungarica
Language English
Size B5
Year of
Foundation
1966
Volumes
per Year
1
Issues
per Year
2
Founder Magyar Tudományos Akadémia  
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-1249 (Print)
ISSN 1588-2691 (Online)

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