Occurrence, molecular diagnosis and suitable time of detection of citrus greening disease in sweet orange

K. Gopal; E. Pradeepthi; V. Gopi; S. Khayum Ahammed; Y. Sreenivasulu; M. Reddy; V. Baranwal; K. Purushotham

doi:10.1556/aphyt.42.2007.1.6

Jump to Content

Acta Phytopathologica et Entomologica Hungarica

Volume/Issue:: Volume 42: Issue 1

Occurrence, molecular diagnosis and suitable time of detection of citrus greening disease in sweet orange

Authors:

K. Gopal

K. Gopal A. N. G. R. Agricultural University All India Coordinated Research Project on Tropical Fruits (Citrus) Tirupati 517 502 Andhra Pradesh India

Search for other papers by K. Gopal in
Current site
Google Scholar
PubMed

E. Pradeepthi

E. Pradeepthi A. N. G. R. Agricultural University All India Coordinated Research Project on Tropical Fruits (Citrus) Tirupati 517 502 Andhra Pradesh India

Search for other papers by E. Pradeepthi in
Current site
Google Scholar
PubMed

V. Gopi

V. Gopi A. N. G. R. Agricultural University All India Coordinated Research Project on Tropical Fruits (Citrus) Tirupati 517 502 Andhra Pradesh India

Search for other papers by V. Gopi in
Current site
Google Scholar
PubMed

S. Khayum Ahammed

S. Khayum Ahammed A. N. G. R. Agricultural University All India Coordinated Research Project on Tropical Fruits (Citrus) Tirupati 517 502 Andhra Pradesh India

Search for other papers by S. Khayum Ahammed in
Current site
Google Scholar
PubMed

Y. Sreenivasulu

Y. Sreenivasulu A. N. G. R. Agricultural University All India Coordinated Research Project on Tropical Fruits (Citrus) Tirupati 517 502 Andhra Pradesh India

Search for other papers by Y. Sreenivasulu in
Current site
Google Scholar
PubMed

M. Reddy

M. Reddy Indian Institute of Horticultural Research Hessaragatta lake post Bangalore 560 089 India

Search for other papers by M. Reddy in
Current site
Google Scholar
PubMed

V. Baranwal

V. Baranwal India Agricultural Research Institute Advanced Centre for Plant Virology Pusa, New Delhi 110 012 India

Search for other papers by V. Baranwal in
Current site
Google Scholar
PubMed

, and

K. Purushotham

K. Purushotham A. N. G. R. Agricultural University All India Coordinated Research Project on Tropical Fruits (Citrus) Tirupati 517 502 Andhra Pradesh India

Search for other papers by K. Purushotham in
Current site
Google Scholar
PubMed

View More View Less

Pages:: 49–58

Online Publication Date:: 31 May 2007

Publication Date:: 01 Jun 2007

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/aphyt.42.2007.1.6

Keywords:: citrus greening; Huanglongbing

Restricted access

Purchase article

USD $30.00

1 year subscription (Individual Only)

USD $542.00

USD $30.00

Click here to view the full Terms and Conditions

USD $542.00

Click here to view the full Terms and Conditions

Huanglongbing (HLB), previously known as citrus greening disease (CGD) is one of the most destructive disease of citrus and responsible for decline of citrus orchards in Andhra Pradesh (A. P) and other citrus growing areas in the country. A preliminary survey on prevalence of HLB associated with citrus was carried out in three major sweet orange growing districts of A. P and the results revealed that a high incidence of 14.5% was recorded in Prakasam district followed by Kadapa (13.18%) and Nellore (12.59%). The DNA extracted from different plant parts of acidlime, i.e. bark, midrib, vein and lamina by CTAB and SS Tris-EDTA methods were subjected to PCR amplification. An amplification product of 1150 bp was observed in bark, midrib and vein. However, no amplification was observed in leaf lamina. Between CTAB and SS Tris-EDTA method, DNA from SS Tris-EDTA method yielded bright band compared to CTAB method. Detection of HLB in sweet orange was done from December 2004 to April 2005 at fortnight interval. It was observed that an excellent amplification product of 1150 bp was observed from December 2004 to February 2005. From March onwards a mild amplification of 1150 bp was observed in both the methods. So it can be concluded that winter season will be the suitable period for detection of the pathogen. Between these two methods of DNA extraction CTAB method was useful for detection of HLB in midrib and veins while SS Tris-EDTA method was useful for detection in bark of sweet orange.

Save
Cite
Email this content

Share Link

Copy this link, or click below to email it to a friend
Email this content
or copy the link directly:

https://akjournals.com/abstract/journals/038/42/1/article-p49.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Acta Phytopathologica et Entomologica Hungarica

Print ISSN:: 0238-1249

Online ISSN:: 1588-2691

Editor-in-Chief

Jenő KONTSCHÁN Centre for Agricultural Research, Hungary

Technical Editor

Ágnes TURÓCI Centre for Agricultural Research, Hungary

Section Editor

K SALÁNKI Centre for Agricultural Research, Hungary

Editorial Board

Z BOZSÓ Centre for Agricultural Research, Hungary

PE CHETVERIKOV Saint-Petersburg State University, Russia

JX CUI Henan Institute of Science and Technology, China

J FODOR Centre for Agricultural Research, Hungary

Z IMREI Centre for Agricultural Research, Hungary

BM KAYDAN Çukurova University, Turkey

L KISS University of Southern Queensland, Australia

V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary

MW NEGM Ibaraki University, Japan

L PALKOVICS Széchenyi István University, Hungary

M POGÁNY Centre for Agricultural Research, Hungary

D RÉDEI National Chung Hsing University, Taiwan

A TOLSTIKOV University of Tyumen, Russia

J VUTS Rothamsted Research, UK

GQ WANG Guangxi University, China

Acta Phytopathologica et Entomologica Hungarica
P.O. Box 102
H-1525 Budapest, Hungary
Phone: (36 1) 487 7534
Fax: (36 1) 487 7555
E-mail: acta@atk.hu

Indexing and Abstracting Services:

Biological Abstracts
BIOSIS Previews
CAB Abstracts
CABELLS Journalytics
Chemical Abstracts
Elsevier GEO Abstracts
Globals Health
Referativnyi Zhurnal
SCOPUS
Zoological Abstracts

2024
Scopus
CiteScore
CiteScore rank
SNIP
Scimago
SJR index	0.188
SJR Q rank	Q4

2023
Scopus
CiteScore	1.1
CiteScore rank	Q4 (Insect Science)
SNIP	0.279
Scimago
SJR index	0.22
SJR Q rank	Q4

Acta Phytopathologica et Entomologica Hungarica
Publication Model	Hybrid
Submission Fee	none
Article Processing Charge	Effective from 1st Feb 2025: 200 EUR/article
Printed Color Illustrations	40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency	World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100%
Further Discounts	Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2025	Online subsscription: 536 EUR / 590 USD Print + online subscription: 626 EUR / 688 USD
Subscription Information	Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title	Individual articles are sold on the displayed price.

Acta Phytopathologica et Entomologica Hungarica
Language	English
Size	B5
Year of Foundation	1966
Volumes per Year	1
Issues per Year	2
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0238-1249 (Print)
ISSN	1588-2691 (Online)