View More View Less
  • 1 CEBAS-CSIC Department of Plant Breeding P.O. Box 164 30100 Espinardo, Murcia Spain
  • 2 CEBAS-CSIC Department of Plant Nutrition P.O. Box 164 30100 Espinardo, Murcia Spain
  • 3 University of Murcia Department of Plant Biology (Plant Physiology) 30100 Murcia Spain
Restricted access

Purchase article

USD  $20.00

1 year subscription (Individual Only)

USD  $542.00

The effect of Plum pox virus (PPV) infection on the response of some antioxidant enzymes was studied in Prunus species showing different susceptibility to PPV. PPV infection produced changes in the antioxidant systems from the Prunus species at subcellular level. In general, in PPV susceptible plants, the infection produced a decrease in some antioxidant enzymes, mainly in chloroplasts. However, in peach cv GF305 (PPV-susceptible) also an increase in class I APX in the three compartments studied as well as an increase in POX took place. In contrast, the PPV-resistant plants showed a general increase of antioxidant enzymes in soluble, chloroplast and apoplastic fractions. Long-term PPV infection produced an oxidative stress only in susceptible plants, as observed by the increase in leaf lipid peroxidation, protein oxidation and electrolyte leakage as well as in apoplastic H 2 O 2 . This H 2 O 2 increase was much higher in the PPV-susceptible than in the PPV-resistant plants. In peach, the increase in apoplastic H 2 O 2 correlated with the rise in NADH-POX and PPO activities. However, in apricot leaves, no increases in apoplastic NADH-POX were observed, and the increases in apoplastic H 2 O 2 correlated with increase in PPO activity in both cv. Real Fino (100%) and cv. SEO (40%), where a rise in SOD was detected, too. The specific inhibitor of NADPH oxidase, diphenylene iodonium (DPI), had only small effects on NADH oxidation, whereas it was inhibited almost completely by 1 mM KCN. Therefore, most of the NADH oxidation in the apoplastic space of Prunus species is due to NADH-POX and not NAD(P)H oxidase. In previous studies and by means of 2D electrophoresis, we showed that PPV infection produced the induction of a thaumatin-like protein as well as the decrease of mandelonitrile lyase (MDL) in peach apoplast. Mandelonitrile lyase catalyses the breakdown of mandelonitrile into benzaldehyde and cyanide. Benzaldehyde can be oxidised easily in vivo to produce benzoic acid, the precursor of salicylic acid. It was suggested that decreased MDL activity may result in a decreased SA biosynthesis in PPV-infected peach plants. It is also possible that a decline in antioxidant enzymes and an increase in ROS could be necessary for the establishment of infection and the replication and spread of some plant viruses, in agreement with results described by other authors. Results suggest that the higher antioxidant capacity showed by resistant SEO plants in comparison to PPV-susceptible plants could be associated, among other factors with their resistance to PPV.

  • Ádam, A., Farkas, T., Somlyai, G., Hevesi, M. and Király Z. (1989): Consequence of O 2 generation during bacterially induced hypersensitive reaction in tobacco: deterioration of membrane lipids. Physiol. Mol. Plant Pathol. 34, 13–26.

    Király Z. , 'Consequence of O2− generation during bacterially induced hypersensitive reaction in tobacco: deterioration of membrane lipids ' (1989 ) 34 Physiol. Mol. Plant Pathol. : 13 -26.

    • Search Google Scholar
  • Allinger, N. L., Cava, M. P., de Jongh, D. C., Johnson, C. R., Lebel, N. A. and Stevens, C. L. (1971): Organic Chemistry. Woorth Publishers, Inc., New York.

    Stevens C. L. , '', in Organic Chemistry , (1971 ) -.

  • Alvarez, M. E., Pennell, R. I., Meijer, P. J., Ishikawa, A., Dixon, R. A. and Lamb, C. (1998): Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92, 773–784.

    Lamb C. , 'Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity ' (1998 ) 92 Cell : 773 -784.

    • Search Google Scholar
  • Appel, H. M. (1993): Phenolics in ecological interaction: the importance of oxidation. J. Chem. Ecol. 19, 1521–1552.

    Appel H. M. , 'Phenolics in ecological interaction: the importance of oxidation ' (1993 ) 19 J. Chem. Ecol. : 1521 -1552.

    • Search Google Scholar
  • Bolwell, G. P., Bindschedler, L. V., Blee, K. A., Butt, V. S., Davies, D. R., Gardner, S. L., Gerrish, C. and Minibayeva, F. (2002): The apoplastic oxidative burst in response to biotic stress in plants: a three component system. J. Exp. Bot. 53, 1367–1376.

    Minibayeva F. , 'The apoplastic oxidative burst in response to biotic stress in plants: a three component system ' (2002 ) 53 J. Exp. Bot. : 1367 -1376.

    • Search Google Scholar
  • Chen, Z., Silva, H. and Klessing, D. F. (1993): Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science 262, 1883–1886.

    Klessing D. F. , 'Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid ' (1993 ) 262 Science : 1883 -1886.

    • Search Google Scholar
  • Chen, D., Juárez, S., Hartweck, L., Alamillo, J. M., Simón-Mateo, C., Pérez, J. J., Fernández-Fernández, M. R., Olszewsky, N. E. and García, J. A. (2005): Identification of secret agents as the O-GlcNAc transferase that participates in Plum pox virus infection. J. Virol. 79, 9381–9387.

    García J. A. , 'Identification of secret agents as the O-GlcNAc transferase that participates in Plum pox virus infection ' (2005 ) 79 J. Virol. : 9381 -9387.

    • Search Google Scholar
  • Clarke, S. F., Guy, P. L., Burritt, D. J. and Jameson, P. E. (2002): Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment. Physiol. Plant. 114, 157–164.

    Jameson P. E. , 'Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment ' (2002 ) 114 Physiol. Plant. : 157 -164.

    • Search Google Scholar
  • De Gara, L., De Pinto, M. C. and Tommasi, F. (2003): The antioxidant systems vis-à-vis reactive oxygen species during plant-pathogen interaction. Plant Physiol. Biochem. 41, 863–870.

    Tommasi F. , 'The antioxidant systems vis-à-vis reactive oxygen species during plant-pathogen interaction ' (2003 ) 41 Plant Physiol. Biochem. : 863 -870.

    • Search Google Scholar
  • Diaz-Vivancos, P., Rubio, M., Mesonero, V., Periago, P. M., Ros-Barceló, A., Martínez-Gómez, P. and Hernández, J. A. (2006): The apoplastic antioxidant system in Prunus : Response to Plum pox virus . J. Exp. Bot. 57, 3813–3824.

    Hernández J. A. , 'The apoplastic antioxidant system in Prunus: Response to Plum pox virus ' (2006 ) 57 J. Exp. Bot. : 3813 -3824.

    • Search Google Scholar
  • Fodor, J., Gullner, G., Ádám, A. L., Barna, B., Kömives, T. and Király, Z. (1997): Local and systemic responses of antioxidants to tobacco mosaic virus infection and to salicylic acid in tobacco. Plant Physiol 114, 1443–1451.

    Király Z. , 'Local and systemic responses of antioxidants to tobacco mosaic virus infection and to salicylic acid in tobacco ' (1997 ) 114 Plant Physiol : 1443 -1451.

    • Search Google Scholar
  • Foyer, C. H. and Harbison, J. (1994): Oxygen metabolism and the regulation of photosynthetic electron transport. In: C. H. Foyer and P. Mullineaux (eds): Causes of Photooxidative Stresses and Amelioration of Defense Systems in Plants. CRC Press, Boca Raton, Florida, USA pp 1–42.

    Harbison J. , '', in Causes of Photooxidative Stresses and Amelioration of Defense Systems in Plants , (1994 ) -.

  • Frew, J., Jones, P. and Scholes, G. (1983): Spectrophotometric determination of hydrogen peroxide and organic hydroperoxides at low concentrations in aqueous solution. Anal. Chim. Acta 155, 130–150.

    Scholes G. , 'Spectrophotometric determination of hydrogen peroxide and organic hydroperoxides at low concentrations in aqueous solution ' (1983 ) 155 Anal. Chim. Acta : 130 -150.

    • Search Google Scholar
  • García-Limones, C., Hervás, A., Navas-Cortés, J. A., Jiménez-Díaz, R. M. and Tena, M. (2002): Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea and Fusarium oxysporum f. sp. ciceris . Physiol. Mol. Plant Pathol. 61, 325–337.

    Tena M. , 'Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea and Fusarium oxysporum f. sp. ciceris ' (2002 ) 61 Physiol. Mol. Plant Pathol. : 325 -337.

    • Search Google Scholar
  • Gullner, G., Tóbiás, I., Fodor, J. and Kömives, T. (1999): Elevation of glutathione level and activation of glutathione-related enzymes affect virus infection in tobacco. Free Rad. Res. 31, S155–161.

    Kömives T. , 'Elevation of glutathione level and activation of glutathione-related enzymes affect virus infection in tobacco ' (1999 ) 31 Free Rad. Res. : S155 -161.

    • Search Google Scholar
  • Hernández, J. A., Olmos, E., Corpas, F. J., Sevilla, F. and del Río, L. A. (1995): Salt-induced oxidative stress in chloroplast of pea plants. Plant Sci. 105, 151–167.

    Río L. A. , 'Salt-induced oxidative stress in chloroplast of pea plants ' (1995 ) 105 Plant Sci. : 151 -167.

    • Search Google Scholar
  • Hernández, J. A., Campillo, A., Jiménez, A., Alarcón, J. J. and Sevilla, F. (1999): Response of antioxidant systems and leaf water relations to NaCl stress in pea plants. New Phytol. 141, 241–251.

    Sevilla F. , 'Response of antioxidant systems and leaf water relations to NaCl stress in pea plants ' (1999 ) 141 New Phytol. : 241 -251.

    • Search Google Scholar
  • Hernández, J. A., Jiménez, A., Mullineaux, P. M. and Sevilla, F. (2000): Tolerance of pea ( Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defences. Plant Cell Environ. 23, 853–862.

    Sevilla F. , 'Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defences ' (2000 ) 23 Plant Cell Environ. : 853 -862.

    • Search Google Scholar
  • Hernández, J. A., Talavera, J. M., Martínez-Gómez, P., Dicenta, F. and Sevilla, F. (2001a): Response of antioxidative enzymes to Plum pox virus in two apricot cultivars. Physiol. Plant. 111, 313–321.

    Sevilla F. , 'Response of antioxidative enzymes to Plum pox virus in two apricot cultivars ' (2001 ) 111 Physiol. Plant. : 313 -321.

    • Search Google Scholar
  • Hernández, J. A., Ferrer, M. A., Jiménez, A., Ros-Barceló, A. and Sevilla, F. (2001b): Antioxidant systems and O 2 .− /H 2 O 2 production in the apoplast of Pisum sativum L. leaves: its relation with NaCl-induced necrotic lesions in minor veins. Plant Physiol. 127, 817–831.

    Sevilla F. , 'Antioxidant systems and O2.−/H2O2 production in the apoplast of Pisum sativum L. leaves: its relation with NaCl-induced necrotic lesions in minor veins ' (2001 ) 127 Plant Physiol. : 817 -831.

    • Search Google Scholar
  • Hernández, J. A., Rubio, M., Olmos, E., Ros-Barceló, A. and Martínez-Gómez, P. (2004): Oxidative stress induced by long-term Plum box virus infection in peach ( Prunus persica L. cv GF305). Physiol. Plant. 122, 486–495.

    Martínez-Gómez P. , 'Oxidative stress induced by long-term Plum box virus infection in peach (Prunus persica L. cv GF305) ' (2004 ) 122 Physiol. Plant. : 486 -495.

    • Search Google Scholar
  • Hernández, J. A., Diaz-Vivancos, P., Rubio, M., Olmos, E., Ros-Barceló, A. and Martínez-Gómez, P. (2006): Long-term PPV infection produces an oxidative stress in a susceptible apricot cultivar but not in a resistant cultivar. Physiol. Plant. 126, 140–152.

    Martínez-Gómez P. , 'Long-term PPV infection produces an oxidative stress in a susceptible apricot cultivar but not in a resistant cultivar ' (2006 ) 126 Physiol. Plant. : 140 -152.

    • Search Google Scholar
  • Hurtado, M. A., Romero, C., Vilanova, S., Abbott, A. G., Llacer, G. and Badenes, M. L. (2002): Genetic linkage maps of two apricot cultivars ( Prunus armeniaca L.) and mapping of PPV (sharka) resistance. Theor. Appl. Gen. 105, 182–191.

    Badenes M. L. , 'Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.) and mapping of PPV (sharka) resistance ' (2002 ) 105 Theor. Appl. Gen. : 182 -191.

    • Search Google Scholar
  • Jiménez, A., Hernández, J. A., Pastori, G., del Río L. A. and Sevilla, F. (1998): The role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves. Plant Physiol. 118, 1327–1335.

    Sevilla F. , 'The role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves ' (1998 ) 118 Plant Physiol. : 1327 -1335.

    • Search Google Scholar
  • Kölber, M. (2001): Workshop on Plum pox virus . Acta Hort. 550, 249–255.

    Kölber M. , 'Workshop on Plum pox virus ' (2001 ) 550 Acta Hort. : 249 -255.

  • Kuwabara, C., Takezawa, D., Shimada, T., Hamada, T., Fujikawa S. and Arakawa, K. (2002): Abscisic acid-and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale . Physiol. Plant. 114, 101–110.

    Arakawa K. , 'Abscisic acid-and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale ' (2002 ) 114 Physiol. Plant. : 101 -110.

    • Search Google Scholar
  • Lansac, M., Eyquard, J. P., Salvador, B., García, J. A., Le Gall, O., Decroocp, V. and Schurdi-Levraud Escalettes, V. (2005): Application of GFP-tagged Plum pox virus to study Prunus -PPV interactions at the whole plant and cellular levels. J. Virol. Meth. 129, 125–133.

    Schurdi-Levraud Escalettes V. , 'Application of GFP-tagged Plum pox virus to study Prunus-PPV interactions at the whole plant and cellular levels ' (2005 ) 129 J. Virol. Meth. : 125 -133.

    • Search Google Scholar
  • Martínez-Gómez, P. and Dicenta, F. (2000): Evaluation of resistance of apricot cultivars to a Spanish isolate of Plum pox potyvirus (PPV). Plant Breeding 119, 179–181.

    Dicenta F. , 'Evaluation of resistance of apricot cultivars to a Spanish isolate of Plum pox potyvirus (PPV) ' (2000 ) 119 Plant Breeding : 179 -181.

    • Search Google Scholar
  • Mittler, R., Herr, E. H., Orvar, B. L., Van Camp, W., Willekens, H., Inzè, D. and Ellis, B. E. (1999): Transgenic tobacco plants with reduced capability to detoxify reactive oxygen intermediates are hyperresponsive to pathogen infection. Proc. Nat. Acad. Sci. USA 96, 14165–14170.

    Ellis B. E. , 'Transgenic tobacco plants with reduced capability to detoxify reactive oxygen intermediates are hyperresponsive to pathogen infection ' (1999 ) 96 Proc. Nat. Acad. Sci. USA : 14165 -14170.

    • Search Google Scholar
  • Patykowski, J. and Urbanek, H. (2003): Activity of enzymes related to H 2 O 2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea . J. Phytopathol. 151, 153–161.

    Urbanek H. , 'Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea ' (2003 ) 151 J. Phytopathol. : 153 -161.

    • Search Google Scholar
  • Pelet, F. and Bovey, R. (1968): Les symptômes de la Sharka sur les pruniers, pruneautiers, abricotiers et pêchers. Agriculture Romande 7, 80–84.

    Bovey R. , 'Les symptômes de la Sharka sur les pruniers, pruneautiers, abricotiers et pêchers ' (1968 ) 7 Agriculture Romande : 80 -84.

    • Search Google Scholar
  • Ros Barceló, A., Gómez-Ros, L. V., Ferrer, M. A., Hernández, J. A. (2006): The apoplastic antioxidant enzymatic system in the wood-forming tissues of trees. Trees-Struct. Funct. 20, 145–156.

    Hernández J. A. , 'The apoplastic antioxidant enzymatic system in the wood-forming tissues of trees ' (2006 ) 20 Trees-Struct. Funct. : 145 -156.

    • Search Google Scholar
  • Sáenz, P., Cervera, M. T., Dallot, S., Quiot, L., Quiot, J. B., Riechman, J. L. and García, J. A. (2000): Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3+6K 1 . J. Gen. Virol. 81, 557–566.

    García J. A. , 'Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3+6K1 ' (2000 ) 81 J. Gen. Virol. : 557 -566.

    • Search Google Scholar
  • Stylianidis, D. C., Sotiropoulos, T. E., Syrgiannidis, G., Therios, I. N., Mainou, A., Karagianni, I. and Asaakidis, A. (2005): Effect of the sharka ( Plum pox virus ) disease on the nutrient status of the apricot ( Prunus armeniaca L.) cultivars ‘Harcot’ and ‘Bebeco’ and expression of symptoms of the physiological disorder tip burn on fruits of the cv. ‘Bebeco’. Eur. J. Hort. Sci. 70, 121–124.

    Asaakidis A. , 'Effect of the sharka (Plum pox virus) disease on the nutrient status of the apricot (Prunus armeniaca L.) cultivars ‘Harcot’ and ‘Bebeco’ and expression of symptoms of the physiological disorder tip burn on fruits of the cv. ‘Bebeco’ ' (2005 ) 70 Eur. J. Hort. Sci. : 121 -124.

    • Search Google Scholar
  • Schwarz, K. B. (1996): Oxidative stress during viral infection: a review. Free Radical Biol. Med. 21, 641–649.

    Schwarz K. B. , 'Oxidative stress during viral infection: a review ' (1996 ) 21 Free Radical Biol. Med. : 641 -649.

    • Search Google Scholar
  • Swain, E. and Poulton J. E. (1994): Immunocytochemical localization of prunasin hydrolase and mandelonitrile lyase in stem and leaves of Prunus serotina . Plant Physiol. 106, 1285–1291

    Poulton J. E. , 'Immunocytochemical localization of prunasin hydrolase and mandelonitrile lyase in stem and leaves of Prunus serotina ' (1994 ) 106 Plant Physiol. : 1285 -1291.

    • Search Google Scholar
  • Vanacker, H., Harbinson, J., Carver, T. L. W. and Foyer C. H. (1998): Antioxidant defences of the apoplast. Protoplasma 205, 129–140.

    Foyer C. H. , 'Antioxidant defences of the apoplast ' (1998 ) 205 Protoplasma : 129 -140.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Oct 2020 1 0 0
Nov 2020 2 0 0
Dec 2020 0 0 0
Jan 2021 1 0 0
Feb 2021 3 0 0
Mar 2021 1 0 0
Apr 2021 0 0 0