View More View Less
  • 1 Hungarian Academy of Sciences Plant Protection Institute P. O. Box 102 H-1525 Budapest Hungary
  • | 2 St. Stephanus University Department of Genetics and Plant Breeding Páter K. u. 1 H-2103 Gödöllő Hungary
  • | 3 University of South Carolina Department of Biological Sciences Columbia SC 29208 USA
  • | 4 School of Food Science and Environmental Health Dublin Institute of Technology Dublin 1 Ireland
Restricted access

Purchase article

USD  $20.00

1 year subscription (Individual Only)

USD  $542.00

As DNA methylation patterns are inherited (‘epigenetic memory’) gsh I transgenic poplar ( Populus × canescens ) clones (11 ggs and 6 Lgl ) were treated with the DNA demethylating drug DHAC (5,6-dihydro-5′-azacytidine hydrochloride) at 10 −4 M for 7 days in order to study acquired plant molecular defense mechanisms in novel plant sources. In this study, the response of relative gene expression levels of transgene gsh I and poplar gene gsh 1 to DHAC treatment were analyzed by qRT-PCR ( q uantitative r everse t ranscriptase PCR). High expression levels of transgene gsh I were observed in the 6 Lgl clone (13.5-fold increase) compared to 11 ggs (1.0) sample. The expression level doubled (1.8-fold increase) in the DHAC-treated 6 Lgl samples but not in the 11 ggs clone (0.4-fold). Contrary to this, the relative copy number of transgene gsh I in the 6 Lgl clone was found to be 60% less (1.0) than in the 11 ggs sample (1.6). Relative expression level of endogenous poplar gene gsh 1 showed significantly higher responsiveness to DHAC-induced demethylation than the transgene gsh I with the highest expression level in the untransformed WT poplar (19.7-fold increase) compared to transformed clones of 6 Lgl (8.7-fold increase) and 11 ggs (2.5-fold increase), respectively. Competition in the reactivation capacity between transgene gsh I and poplar gsh 1 of 6 Lgl clone was also observed as the relative gene expression level of transgene gsh I increased from a high relative expression level (13.5) up by about twofold (1.8 times) rate (to 23.7) compared to poplar gsh 1 gene that increased by an 8.7 increment from a lower level (1.6 rel. expression) to 13.9.

  • Anand, A., Trick, H. N., Gill, B. S. and Muthukrishnan, S. (2003): Stable transgene expression and random gene silencing in wheat. Plant Biotechnology Journal 1, 241–251.

    Muthukrishnan S. , 'Stable transgene expression and random gene silencing in wheat ' (2003 ) 1 Plant Biotechnology Journal : 241 -251.

    • Search Google Scholar
  • Arisi, A. C. M., Noctor, G., Foyer, C. H. and Jouanin, L. (1997): Modification of thiol contents in poplars ( Populus tremula × P. alba ) overexpressing enzymes involved in glutathione synthesis. Planta 203, 362–372.

    Jouanin L. , 'Modification of thiol contents in poplars (Populus tremula × P. alba) overexpressing enzymes involved in glutathione synthesis ' (1997 ) 203 Planta : 362 -372.

    • Search Google Scholar
  • Barna, B., Ádám, L. and Király, Z. (1993): Juvenility and resistance of a superoxide-tolerant plant to diseases and other stresses. Naturwissenschaften 80, 420–422.

    Király Z. , 'Juvenility and resistance of a superoxide-tolerant plant to diseases and other stresses ' (1993 ) 80 Naturwissenschaften : 420 -422.

    • Search Google Scholar
  • Baumberger, N. and Baulcombe, D. C. (2005): Arabidopsis ARGONAUTE1 is an RNA slicer that selectively recruit microRNAs and short interfering RNAs. Proc. Natl. Acad. Sci. USA 102, 11928–11933.

    Baulcombe D. C. , 'Arabidopsis ARGONAUTE1 is an RNA slicer that selectively recruit microRNAs and short interfering RNAs ' (2005 ) 102 Proc. Natl. Acad. Sci. USA : 11928 -11933.

    • Search Google Scholar
  • Bird, A. (2002): DNA methylation patterns and epigenetic memory. Genes and Development 16, 6–21.

    Bird A. , 'DNA methylation patterns and epigenetic memory ' (2002 ) 16 Genes and Development : 6 -21.

    • Search Google Scholar
  • Bittsánszky, A., Kőmíves, T., Gullner, G., Gyulai, G., Kiss, J., Heszky, L., Radimszky, L. and Rennenberg, H. (2005): Ability of transgenic poplars with elevated glutathione content to tolerate Zinc (2+) stress. Environment International 31, 251–254.

    Rennenberg H. , 'Ability of transgenic poplars with elevated glutathione content to tolerate Zinc (2+) stress ' (2005 ) 31 Environment International : 251 -254.

    • Search Google Scholar
  • Bittsánszky, A., Gyulai, G., Humphreys, M., Gullner, G., Csintalan, Z., Kiss, J., Szabó, Z., Lágler, R., Tóth, Z., Rennenberg, H., Heszky, L. and Kőmíves, T. (2006): RT-PCR analysis and stress response capacity of transgenic gshI-poplar chones ( Populus × canescens ) in response to paraquat exposure. Z. Naturforschung 61c, 699–730.

    Kőmíves T. , 'RT-PCR analysis and stress response capacity of transgenic gshI-poplar chones (Populus × canescens) in response to paraquat exposure ' (2006 ) 61c Z. Naturforschung : 699 -730.

    • Search Google Scholar
  • Bruce, B. D. (2001): The paradox of plastid transit peptides: conservation of function despite divergence in primary structure. Biochimica Biophysica Acta 1541, 2–21.

    Bruce B. D. , 'The paradox of plastid transit peptides: conservation of function despite divergence in primary structure ' (2001 ) 1541 Biochimica Biophysica Acta : 2 -21.

    • Search Google Scholar
  • Cao, X., Springer, N. M., Muszynskii, M. G., Phillips, R. L., Kaeppler, S. and Jacobsen, S. E. (2000): Conserved plant genes with similarity to mammalian de novo DNA methyltransferases. Proc. Natl. Acad. Sci. USA 97, 4979–4984.

    Jacobsen S. E. , 'Conserved plant genes with similarity to mammalian de novo DNA methyltransferases ' (2000 ) 97 Proc. Natl. Acad. Sci. USA : 4979 -4984.

    • Search Google Scholar
  • Castilho, A., Neves, N., Rufini-Castiglione, M., Viegas, W. and Heslop-Harrison, J. S. (1999): 5-Methylcytosine distribution and genome organization in Triticale before and after treatment with 5-azacytidine. J. Cell Science 112, 4397–4404.

    Heslop-Harrison J. S. , '5-Methylcytosine distribution and genome organization in Triticale before and after treatment with 5-azacytidine ' (1999 ) 112 J. Cell Science : 4397 -4404.

    • Search Google Scholar
  • Chen, Z. J. and Pikaard, C. S. (1997): Epigenetic silencing of RNA polymerase I transcription: a role for DNA methylation and histone modification in nucleolar dominance. Genes and Development 11, 2124–2136.

    Pikaard C. S. , 'Epigenetic silencing of RNA polymerase I transcription: a role for DNA methylation and histone modification in nucleolar dominance ' (1997 ) 11 Genes and Development : 2124 -2136.

    • Search Google Scholar
  • Cheng, X. and Roberts, R. J. (2001): AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucleic Acid Res. 29, 3784–3795.

    Roberts R. J. , 'AdoMet-dependent methylation, DNA methyltransferases and base flipping ' (2001 ) 29 Nucleic Acid Res. : 3784 -3795.

    • Search Google Scholar
  • Creissen, G., Broadbent, P., Stevens, R., Wellburn, A. R. and Mullineaux, P. (1996): Manipulation of glutathione metabolism in transgenic plants. Biochemical Society Transactions 24, 465–469.

    Mullineaux P. , 'Manipulation of glutathione metabolism in transgenic plants ' (1996 ) 24 Biochemical Society Transactions : 465 -469.

    • Search Google Scholar
  • Elmayan, T. and Vaucheret, H. (1996): Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally. Plant J. 9, 787–797.

    Vaucheret H. , 'Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally ' (1996 ) 9 Plant J. : 787 -797.

    • Search Google Scholar
  • Fan, J., Kodama, E., Koh, Y., Nakao, M. and Matsuoka, M. (2005): Halogenated thymidine analogues restore the expression of silenced genes without demethylation. Cancer Res. 65, 6927–6933.

    Matsuoka M. , 'Halogenated thymidine analogues restore the expression of silenced genes without demethylation ' (2005 ) 65 Cancer Res. : 6927 -6933.

    • Search Google Scholar
  • Finnegan, E. J., Genger, R. K., Kovac, J., Peacock, W. J. and Dennis, E. S. (1998): DNA methylation and the promotion of flowering by vernalization. Proc. Natl. Acad. Sci. USA 95, 5824–5829.

    Dennis E. S. , 'DNA methylation and the promotion of flowering by vernalization ' (1998 ) 95 Proc. Natl. Acad. Sci. USA : 5824 -5829.

    • Search Google Scholar
  • Goffin, J. and Eisenhauer, E. (2002): DNA methyltransferase inhibitors-state of the art. Annals of Oncology 13, 1699–1716.

    Eisenhauer E. , 'DNA methyltransferase inhibitors-state of the art ' (2002 ) 13 Annals of Oncology : 1699 -1716.

    • Search Google Scholar
  • Gullner, G., Gyulai, G., Bittsánszky, A., Kiss, J., Heszky, L. and Kömíves, T. (2005): Enhanced inducibility of glutathione S-transferase activity by paraquat in poplar leaf discs in the presence of sucrose. Phyton 45, 39–44.

    Kömíves T. , 'Enhanced inducibility of glutathione S-transferase activity by paraquat in poplar leaf discs in the presence of sucrose ' (2005 ) 45 Phyton : 39 -44.

    • Search Google Scholar
  • Gyulai, G., Jekkel, Z., Kiss, E., Kiss, J. and Heszky, L. E. (1995): A selective auxin and cytokinin bioassay based on root and shoot formation in vitro. J. Plant Physiol. 145, 379–382.

    Heszky L. E. , 'A selective auxin and cytokinin bioassay based on root and shoot formation in vitro ' (1995 ) 145 J. Plant Physiol. : 379 -382.

    • Search Google Scholar
  • Gyulai, G., Humphreys, M., Bittsánszky, A., Skøt, K., Kiss, J., Skøt, L., Gullner, G., Heywood, S., Szabó, Z., Lovatt, A., Radimszky, L., Roderick, H., Abberton, M., Rennenberg, H., Kőmíves, T. and Heszky, L. (2005): AFLP analysis and improved phytoextraction capacity of transgenic gsh I-poplar clones ( Populus canescens L.) in vitro . Z. Naturforschung 60c, 300–306.

    Heszky L. , 'AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus canescens L.) in vitro ' (2005 ) 60c Z. Naturforschung : 300 -306.

    • Search Google Scholar
  • Horváth, E., Szalai, G., Janda, T., Páldi, E., Rácz, I. and Lásztity, D. (2003): Effect of vernalisation and 5-azacytidine on the methylation level of DNA in wheat ( Triticum aestivum L., cv. Martonvásár 15). Plant Science 165, 689–692.

    Lásztity D. , 'Effect of vernalisation and 5-azacytidine on the methylation level of DNA in wheat (Triticum aestivum L., cv. Martonvásár 15) ' (2003 ) 165 Plant Science : 689 -692.

    • Search Google Scholar
  • Issa, J. P. and Kantarjian, H. (2005): Azacitidine. Nature Reviews Drug Discovery. May, Suppl, S6–S7.

  • Kiss, J., Kondrák, M., Törjék, O., Kiss, E., Gyulai, G., Mázik-Tőkei, K. and Heszky, L. (2001): Morphological and RAPD analysis of poplar trees of anther culture origin. Euphytica 118, 213–221.

    Heszky L. , 'Morphological and RAPD analysis of poplar trees of anther culture origin ' (2001 ) 118 Euphytica : 213 -221.

    • Search Google Scholar
  • Kőmíves, T., Gullner, G. and Király, Z. (1998): Role of glutathione and glutathione-related enzymes in response of plants to environmental stress. Stress of Life. From Molecules to Man 851, 251–258.

    Király Z. , 'Role of glutathione and glutathione-related enzymes in response of plants to environmental stress ' (1998 ) 851 Stress of Life. From Molecules to Man : 251 -258.

    • Search Google Scholar
  • Kumpatla, S. P., Teng, W., Buchholz, W. G. and Hall, T. C. (1997): Epigenetic transcriptional silencing and 5-azacitidne-mediated reactivation of a complex transgene in rice. Plant Physiol. 115, 361–373.

    Hall T. C. , 'Epigenetic transcriptional silencing and 5-azacitidne-mediated reactivation of a complex transgene in rice ' (1997 ) 115 Plant Physiol. : 361 -373.

    • Search Google Scholar
  • Leple, J. C., Brasileiro, A. C. M., Michel, N. F., Delmotte, F. and Jouanin, L. (1992): Transgenic poplars — Expression of chimeric genes using 4 different constructs. Plant Cell Reports 11, 137–141.

    Jouanin L. , 'Transgenic poplars — Expression of chimeric genes using 4 different constructs ' (1992 ) 11 Plant Cell Reports : 137 -141.

    • Search Google Scholar
  • Linn, F., Heidmann, I., Saedler, H. and Meyer, P. (1990): Epigenetic changes in the expression of the maize A1 gene in Petunia hybrida : role of numbers of integrated gene copies and state of methylation. Molecular and General Genetics 222, 329–336.

    Meyer P. , 'Epigenetic changes in the expression of the maize A1 gene in Petunia hybrida: role of numbers of integrated gene copies and state of methylation ' (1990 ) 222 Molecular and General Genetics : 329 -336.

    • Search Google Scholar
  • Livak, K. J. and Schmittgen, T. D. (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2 DD Ct method. Methods 25, 402–408.

    Schmittgen T. D. , 'Analysis of relative gene expression data using real-time quantitative PCR and the 2−DDCt method ' (2001 ) 25 Methods : 402 -408.

    • Search Google Scholar
  • Lloyd, G. B. and McCown, B. H. (1980): Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia , by use of shoot-tip culture. Proc. Inter. Plant Prop. Soc. 30, 421–427.

    McCown B. H. , 'Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture ' (1980 ) 30 Proc. Inter. Plant Prop. Soc. : 421 -427.

    • Search Google Scholar
  • Murashige, T. and Skoog, F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

    Skoog F. , 'A revised medium for rapid growth and bioassays with tobacco tissue cultures ' (1962 ) 15 Physiol. Plant. : 473 -497.

    • Search Google Scholar
  • Noctor, G., Arisi, A. C. M., Jouanin, L., Kunert, K. J., Rennenberg, H. and Foyer, C. H. (1998a): Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants. J. Exp. Botany 49, 623–647.

    Foyer C. H. , 'Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants ' (1998 ) 49 J. Exp. Botany : 623 -647.

    • Search Google Scholar
  • Noctor, G., Arisi, A. C. M., Jouanin, L. and Foyer, C. H. (1998b): Manipulation of glutathione and amino acid biosynthesis in the chloroplast. Plant Physiology 118, 471–482.

    Foyer C. H. , 'Manipulation of glutathione and amino acid biosynthesis in the chloroplast ' (1998 ) 118 Plant Physiology : 471 -482.

    • Search Google Scholar
  • Prakash, P. A., Kush, A., Lakshmanan, P. and Kumar, P. P. (2003): Cytosine methylation occurs in a CDC48 homologue and a MADS-box gene during adventitious shoot induction in Petunia leaf explants. J. Exp. Botany 54, 1361–1371.

    Kumar P. P. , 'Cytosine methylation occurs in a CDC48 homologue and a MADS-box gene during adventitious shoot induction in Petunia leaf explants ' (2003 ) 54 J. Exp. Botany : 1361 -1371.

    • Search Google Scholar
  • Rozen, S. and Skaletsky, H. J. (1997): Primer3 on the WWW for general users and for biologist programmers. In: S. Krawetz and S. Misener (eds): Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp. 365–386.

    Skaletsky H. J. , '', in Bioinformatics Methods and Protocols: Methods in Molecular Biology , (1997 ) -.

  • Wolffe, A. P. and Matzke, M. A. (1999): Epigenetics: Regulation through repression. Science 286, 481–486.

    Matzke M. A. , 'Epigenetics: Regulation through repression ' (1999 ) 286 Science : 481 -486.

  • Yang, G., Lee, Y. H., Jiang, Y., Kumpatla, S. P. and Hall, T. C. (2005): Organization, not duplication, triggers silencing in a complex transgene locus in rice. Plant Mol. Biol. 58, 351–366.

    Hall T. C. , 'Organization, not duplication, triggers silencing in a complex transgene locus in rice ' (2005 ) 58 Plant Mol. Biol. : 351 -366.

    • Search Google Scholar
  • Zardo, G., Marenzi, S., Perilli, M. and Caiafa, P. (1999): Inhibition of poly(ADP-ribosyl)ation introduces an anomalous methylation pattern in transfected foreign DNA. The FASEB Journal 13, 1518–1522.

    Caiafa P. , 'Inhibition of poly(ADP-ribosyl)ation introduces an anomalous methylation pattern in transfected foreign DNA ' (1999 ) 13 The FASEB Journal : 1518 -1522.

    • Search Google Scholar
Submit Your Manuscript
 
The author instruction is available in PDF.
Please, download the file from HERE.

Editor-in-Chief

Technical Editor

  • Ágnes TURÓCI Centre for Agricultural Research, Hungary

Section Editor

  • K SALÁNKI Centre for Agricultural Research, Hungary

Editorial Board

Acta Phytopathologica et Entomologica Hungarica
P.O. Box 102
H-1525 Budapest, Hungary
Phone: (36 1) 487 7534
Fax: (36 1) 487 7555
E-mail: acta@atk.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Elsevier GEO Abstracts
  • Globals Health
  • Referativnyi Zhurnal
  • SCOPUS
  • Zoological Abstracts

 

 

2020  
Scimago
H-index
20
Scimago
Journal Rank
0,185
Scimago
Quartile Score
Insect Science Q4
Plant Science Q4
Scopus
Cite Score
75/98=0,8
Scopus
Cite Score Rank
Insect Science 129/153 (Q4)
Plant Science 353/445 (Q4)
Scopus
SNIP
0,438
Scopus
Cites
313
Scopus
Documents
20
Days from submission to acceptance 64
Days from acceptance to publication 209
Acceptance
Rate
48%

 

2019  
Scimago
H-index
19
Scimago
Journal Rank
0,177
Scimago
Quartile Score
Insect Science Q4
Plant Science Q4
Scopus
Cite Score
66/103=0,6
Scopus
Cite Score Rank
Insect Science 125/142 (Q4)
Plant Science 344/431 (Q4)
Scopus
SNIP
0,240
Scopus
Cites
212
Scopus
Documents
24
Acceptance
Rate
35%

 

Acta Phytopathologica et Entomologica Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 900 EUR/article
Printed Color Illustrations 40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2021 Online subsscription: 450 EUR / 562 USD
Print + online subscription: 524 EUR / 654 USD
Subscription fee 2022 Online subsscription: 460 EUR / 576 USD
Print + online subscription: 536 EUR / 670 USD
Subscription Information Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Phytopathologica et Entomologica Hungarica
Language English
Size B5
Year of
Foundation
1966
Publication
Programme
2020 Volume 55
Volumes
per Year
1
Issues
per Year
2
Founder Magyar Tudományos Akadémia  
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0238-1249 (Print)
ISSN 1588-2691 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2021 2 0 0
Jul 2021 2 0 0
Aug 2021 19 0 0
Sep 2021 2 0 0
Oct 2021 2 0 0
Nov 2021 0 0 0
Dec 2021 0 0 0