Towards the map based cloning of a recessive Fusarium resistance determinant from diploid Medicago sativa

Márta Balogh; Krisztina Miró; Ágnes Dalmadi; Gábor Deák; Tünde Petrovics; Brigitta Dudás; Péter Kiss; Júlia Jakab; György Kiss

doi:10.1556/aphyt.42.2007.2.10

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Acta Phytopathologica et Entomologica Hungarica

Volume/Issue:: Volume 42: Issue 2

Towards the map based cloning of a recessive Fusarium resistance determinant from diploid Medicago sativa

Authors:

Márta Balogh

Márta Balogh Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary

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Krisztina Miró

Krisztina Miró Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary

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Ágnes Dalmadi

Ágnes Dalmadi Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary

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Gábor Deák

Gábor Deák Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary

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Tünde Petrovics

Tünde Petrovics Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary

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Brigitta Dudás

Brigitta Dudás Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary

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Péter Kiss

Péter Kiss Biological Research Center of the Hungarian Academy of Sciences Temesvári krt. 62 H-6726 Szeged Hungary

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Júlia Jakab

Júlia Jakab Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary

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, and

György Kiss

György Kiss

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Pages:: 279–289

Online Publication Date:: 26 Nov 2007

Publication Date:: 01 Dec 2007

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/aphyt.42.2007.2.10

Keywords:: genetic mapping; map based cloning; pathogen interaction

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A monogenic recessive Fusarium resistance determinant was identified in a diploid Medicago sativa population. The Fusarium susceptible and resistant phenotypes of the individuals in an F2 segregation population was inferred from a biological test using injured root test. The resulting phenotypes were used to position the Fusarium resistance determinant on linkage group 6 of the Medicago sativa genetic map. Using the DNA based molecular markers genome walk was initiated from both sides of the genetic region constructing two contigs which are about 0.5 centiMorgan (∼500 kilobasepairs) apart. The introduction of this genetic determinant into tetraploid alfalfa cultivars was unsuccessful since only triploid and pentaploid hybrids could be recovered from the 4×-2× crosses. The isolation of the gene and establishment of the function of this recessive Fusarium determinant is under progress.

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Acta Phytopathologica et Entomologica Hungarica

Print ISSN:: 0238-1249

Online ISSN:: 1588-2691

Editor-in-Chief

Jenő KONTSCHÁN Centre for Agricultural Research, Hungary

Technical Editor

Ágnes TURÓCI Centre for Agricultural Research, Hungary

Section Editor

K SALÁNKI Centre for Agricultural Research, Hungary

Editorial Board

Z BOZSÓ Centre for Agricultural Research, Hungary

PE CHETVERIKOV Saint-Petersburg State University, Russia

JX CUI Henan Institute of Science and Technology, China

J FODOR Centre for Agricultural Research, Hungary

Z IMREI Centre for Agricultural Research, Hungary

BM KAYDAN Çukurova University, Turkey

L KISS University of Southern Queensland, Australia

V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary

MW NEGM Ibaraki University, Japan

L PALKOVICS Széchenyi István University, Hungary

M POGÁNY Centre for Agricultural Research, Hungary

D RÉDEI National Chung Hsing University, Taiwan

A TOLSTIKOV University of Tyumen, Russia

J VUTS Rothamsted Research, UK

GQ WANG Guangxi University, China

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Acta Phytopathologica et Entomologica Hungarica
Language	English
Size	B5
Year of Foundation	1966
Volumes per Year	1
Issues per Year	2
Founder	Magyar Tudományos Akadémia
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