View More View Less
  • 1 Agricultural Biotechnology Center of the Ministry of Agriculture and Rural Development Szent-Györgyi A. u. 4 H-2100 Gödöllő Hungary
  • | 2 Biological Research Center of the Hungarian Academy of Sciences Temesvári krt. 62 H-6726 Szeged Hungary
Restricted access

Purchase article

USD  $20.00

1 year subscription (Individual Only)

USD  $542.00

Cross Mark

A monogenic recessive Fusarium resistance determinant was identified in a diploid Medicago sativa population. The Fusarium susceptible and resistant phenotypes of the individuals in an F2 segregation population was inferred from a biological test using injured root test. The resulting phenotypes were used to position the Fusarium resistance determinant on linkage group 6 of the Medicago sativa genetic map. Using the DNA based molecular markers genome walk was initiated from both sides of the genetic region constructing two contigs which are about 0.5 centiMorgan (∼500 kilobasepairs) apart. The introduction of this genetic determinant into tetraploid alfalfa cultivars was unsuccessful since only triploid and pentaploid hybrids could be recovered from the 4×-2× crosses. The isolation of the gene and establishment of the function of this recessive Fusarium determinant is under progress.

  • Bingham, E. T. (1969): Haploids from cultivated alfalfa. Nature 221, 865–866.

    Bingham E. T. , 'Haploids from cultivated alfalfa ' (1969 ) 221 Nature : 865 -866.

  • Bingham, E. T. (1971): Isolation of haploids of tetraploid alfalfa. Crop Sci. 11, 433–435.

    Bingham E. T. , 'Isolation of haploids of tetraploid alfalfa ' (1971 ) 11 Crop Sci. : 433 -435.

    • Search Google Scholar
  • Bingham, E. T. and McCoy, T. J. (1979): Cultivated alfalfa at the diploid level: origin, reproductive stability, and yield of seed and forage. Crop Sci. 19, 97–100.

    McCoy T. J. , 'Cultivated alfalfa at the diploid level: origin, reproductive stability, and yield of seed and forage ' (1979 ) 19 Crop Sci. : 97 -100.

    • Search Google Scholar
  • Büschges, R., Hollricher, K., Panstruga, R., Simons, G., Wolter, M., Frijters, A., van Daelen, R., van der Lee, T., Diergaarde, P., Groenendijk, J., Topsch, S., Vos, P., Salamini, F. and Schulze-Lefert, P. (1997): The barley mlo gene: a novel control element of plant pathogen resistance. Cell 88, 695–705.

    Schulze-Lefert P. , 'The barley mlo gene: a novel control element of plant pathogen resistance ' (1997 ) 88 Cell : 695 -705.

    • Search Google Scholar
  • Choi, H. K., Kim, D. J., Uhm, T., Limpens, E., Baek, J-M., Lim, H., Kaló, P., Penmetsa, R. V., Seres, A., Kulikova, O., Bisseling, T., Kiss, G. B. and Cook, D. R. (2004): A sequence-based genetic map of Medicago truncatula and comparison of marker co-linearity with Medicago sativa . Genetics 166, 1463–1502.

    Cook D. R. , 'A sequence-based genetic map of Medicago truncatula and comparison of marker co-linearity with Medicago sativa ' (2004 ) 166 Genetics : 1463 -1502.

    • Search Google Scholar
  • Cook, D. (1999): Medicago truncatula — a model in the making! Curr. Opin. Plant Biol. 2, 301–304.

    Cook D. , 'Medicago truncatula — a model in the making! ' (1999 ) 2 Curr. Opin. Plant Biol. : 301 -304.

    • Search Google Scholar
  • Dalmadi, Á., Jakab, J., Kaló, P., Kereszt, A., Saskői, A., Petrovics, T., Deák, G. and Kiss, G. B. (2007): Dwarfism of diploid M. sativa is caused by a mutation in the gibberellin 3-β-hydroxylase gene. (in preparation)

  • Dolezel, J., Binarová, P. and Lucretti, S. (1989): Analysis of nuclear DNA content in plant cells by flow cytometry. Biol. Plant. 31, 113–120.

    Lucretti S. , 'Analysis of nuclear DNA content in plant cells by flow cytometry ' (1989 ) 31 Biol. Plant. : 113 -120.

    • Search Google Scholar
  • Endre, G., Kaló, P., Hangyel Tárczy, M., Csanádi, G. and Kiss, G. B. (1996): Reducing the tetraploid non-nodulating alfalfa ( Medicago sativa ) MnNC-1008(NN) germ plasm to the diploid level. Theor. Appl. Genet. 93, 1061–1065.

    Kiss G. B. , 'Reducing the tetraploid non-nodulating alfalfa (Medicago sativa) MnNC-1008(NN) germ plasm to the diploid level ' (1996 ) 93 Theor. Appl. Genet. : 1061 -1065.

    • Search Google Scholar
  • Kaló, P., Endre, G., Zimányi, L., Csanádi, G. and Kiss, G. B. (2000): Construction of an improved linkage map of diploid alfalfa ( Medicago sativa ). Theor. Appl. Genet. 100, 641–657.

    Kiss G. B. , 'Construction of an improved linkage map of diploid alfalfa (Medicago sativa) ' (2000 ) 100 Theor. Appl. Genet. : 641 -657.

    • Search Google Scholar
  • Kiss, G. B., Csanádi, G., Kálmán, K., Kaló, P. and Ökrész, L. (1993): Construction of a basic genetic map of Medicago using RFLP, RAPD, Isozyme and morphological markers. Mol. Gen. Genet. 238, 129–137.

    Ökrész L. , 'Construction of a basic genetic map of Medicago using RFLP, RAPD, Isozyme and morphological markers ' (1993 ) 238 Mol. Gen. Genet. : 129 -137.

    • Search Google Scholar
  • Kiss, G. B., Kereszt, A., Kiss, P. and Endre, G. (1998): Colormapping: a non-mathematical procedure for genetic mapping. Acta Biologica Hungarica 49, 47–64.

    Endre G. , 'Colormapping: a non-mathematical procedure for genetic mapping ' (1998 ) 49 Acta Biologica Hungarica : 47 -64.

    • Search Google Scholar
  • Mun, J. H., Kim, D. J., Choi, H. K., Gish, J., Debelle, F., Mudge, J., Denny, R., Endre, G., Saurat, O., Dudez, A. M., Kiss, G. B., Roe, B., Young, N. D. and Cook, D. R. (2006): Distribution of microsatellites in the genome of Medicago truncatula : a resource of genetic markers that integrate genetic and physical maps. Genetics 172, 2541–2555.

    Cook D. R. , 'Distribution of microsatellites in the genome of Medicago truncatula: a resource of genetic markers that integrate genetic and physical maps ' (2006 ) 172 Genetics : 2541 -2555.

    • Search Google Scholar
  • Weimer, J. L. (1928): A wilt disease of alfalfa caused by Fusarium oxysporum var. medicaginis , n. var. J. of Agricultural Res. 37, 419–433.

    Weimer J. L. , 'A wilt disease of alfalfa caused by Fusarium oxysporum var. medicaginis, n. var ' (1928 ) 37 J. of Agricultural Res. : 419 -433.

    • Search Google Scholar
All Time Past Year Past 30 Days
Abstract Views 14 14 1
Full Text Views 8 5 0
PDF Downloads 1 0 0