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  • 1 Szent István University Agricultural Biotechnology Center, Mycology Group of the Hungarian Academy of Sciences, Institute of Plant Protection Páter K. u. 1 H-2103 Gödöllő Hungary
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Fphog1 , a HOG-type mitogen-activated protein kinase (MAPK) encoding gene of Fusarium proliferatum was constitutively expressed in all types of fungal cells. Δ Fphog1 mutants grew normally on artificial media; sporulation and spore germination were also normal. The mating capability of M24, a representative strain of the Δ Fphog1 mutants, showed no detectable decline, indicating that this HOG-type MAPK gene is dispensable for growth and reproduction under optimal culture conditions. Strain M24 had increased tolerance to vinclozoline and fludioxonil fungicides. Invasive growth of the wild type and three Δ Fphog1 gene replacement mutants (M21, M24, M36) were assessed on tomato fruits. All strains behaved similarly, i.e. they produced visible symptoms on the second day after infection, and produced ∼3 cm lesion, overgrown by fungal mycelium after six days of incubation. These data suggest that the HOG-type MAPK pathway is not required for the invasive growth of this fungus.

  • Ádám, A. L., Kohut, G. and Láday, M. (2005): Identification of new molecular hallmarks for YSAPK MAPKs: Application for cloning strategies in different fungal filamentous species. Acta Phytopathol. Entomol. Hung. 40, 233–249.

    Láday M. , 'Identification of new molecular hallmarks for YSAPK MAPKs: Application for cloning strategies in different fungal filamentous species ' (2005 ) 40 Acta Phytopathol. Entomol. Hung. : 233 -249.

    • Search Google Scholar
  • Ádám, A. L., Pike, S., Hoyos, M. E., Stone, J. M., Walker, J. C. and Novacky, A. (1997): Rapid and transient activation of an MBP kinase in tobacco leaves treated with harpin from Erwinia amylovora . Plant Physiol. 115, 853–861.

    Novacky A. , 'Rapid and transient activation of an MBP kinase in tobacco leaves treated with harpin from Erwinia amylovora ' (1997 ) 115 Plant Physiol. : 853 -861.

    • Search Google Scholar
  • Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Millwer, W. and Lipman, D. J. (1997): Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402.

    Lipman D. J. , 'Gapped BLAST and PSI-BLAST: A new generation of protein database search programs ' (1997 ) 25 Nucleic Acids Res. : 3389 -3402.

    • Search Google Scholar
  • Antal, Z., Rascle, C., Fevre, M. and Bruel, C. (2004): Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags. Curr. Genet. 46, 240–246.

    Bruel C. , 'Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags ' (2004 ) 46 Curr. Genet. : 240 -246.

    • Search Google Scholar
  • Brewster, J. L., de Valoir, T., Dwyer, N. D., Winter, E. and Gustin, M. C. (1993): An osmosensing signal transduction pathway in yeast. Science 259, 1760–1763.

    Gustin M. C. , 'An osmosensing signal transduction pathway in yeast ' (1993 ) 259 Science : 1760 -1763.

    • Search Google Scholar
  • Cappellini, R. and Peterson, J. L. (1965): Macroconidium formation in submerged cultures by a non-sporulating strain of Gibberella zeae . Mycologia 57, 962–966.

    Peterson J. L. , 'Macroconidium formation in submerged cultures by a non-sporulating strain of Gibberella zeae ' (1965 ) 57 Mycologia : 962 -966.

    • Search Google Scholar
  • Di Pietro, A., Garcia-Maceira, F. I., Meglecz, E. and Roncero, M. I. G. (2001): A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis. Mol. Microbiol. 39, 1140–1152.

    Roncero M. I. G. , 'A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis ' (2001 ) 39 Mol. Microbiol. : 1140 -1152.

    • Search Google Scholar
  • Dixon, K. P., Xu, J. R., Smirnoff, N. and Talbot, N. J. (1999): Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection in Magnaporthe grisea . Plant Cell 11, 2045–2058.

    Talbot N. J. , 'Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection in Magnaporthe grisea ' (1999 ) 11 Plant Cell : 2045 -2058.

    • Search Google Scholar
  • Felsenstein, J, (2004): PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle.

    Felsenstein J. , '', in PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author , (2004 ) -.

    • Search Google Scholar
  • Glass, N. L. and Donaldson, G. C. (1995): Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61, 1323–1330.

    Donaldson G. C. , 'Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes ' (1995 ) 61 Appl. Environ. Microbiol. : 1323 -1330.

    • Search Google Scholar
  • Hou, Z., Xue, C., Peng, Y., Katan, T., Kistler, H. C. and Xu J. R. (2002): A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection. Mol. Plant-Microbe Interact. 15, 1119–1127.

    Xu J. R. , 'A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection ' (2002 ) 15 Mol. Plant-Microbe Interact. : 1119 -1127.

    • Search Google Scholar
  • Jenczmionka, N. J., Maier, F. J., Lösch, A. P. and Schäfer, W. (2003): Mating, conidiation and pathogenicity of Fusarium graminearum , the main causal agent of the head-blight disease of wheat, are regulated by the MAP kinase gpmk1 . Curr. Genet. 43, 87–95.

    Schäfer W. , 'Mating, conidiation and pathogenicity of Fusarium graminearum, the main causal agent of the head-blight disease of wheat, are regulated by the MAP kinase gpmk1 ' (2003 ) 43 Curr. Genet. : 87 -95.

    • Search Google Scholar
  • Jenczmionka, N. J. and Schäfer, W. (2005): The Gpmk1 MAP kinase of Fusarium graminearum regulates the induction of specific secreted enzymes. Curr. Genet. 47, 29–36.

    Schäfer W. , 'The Gpmk1 MAP kinase of Fusarium graminearum regulates the induction of specific secreted enzymes ' (2005 ) 47 Curr. Genet. : 29 -36.

    • Search Google Scholar
  • Jeney, A., Béki, E., Mulé, G. and Hornok, L. (2004): Identification of growth stage specific transcript profiles in Fusarium proliferatum ( Gibberella fujikuroi , mating population D) by cDNA-AFLP analysis. Eur. J. Plant Pathol. 110, 619–625.

    Hornok L. , 'Identification of growth stage specific transcript profiles in Fusarium proliferatum (Gibberella fujikuroi, mating population D) by cDNA-AFLP analysis ' (2004 ) 110 Eur. J. Plant Pathol. : 619 -625.

    • Search Google Scholar
  • Kerényi, Z., Oláh, B., Jeney, A., Hornok, L. and Leslie, J. F. (2006): The homologue of het-c of Neurospora crassa lacks vegetative compatibility function in Fusarium proliferatum . Appl. Environ. Microbiol. 72, 6527–6532.

    Leslie J. F. , 'The homologue of het-c of Neurospora crassa lacks vegetative compatibility function in Fusarium proliferatum ' (2006 ) 72 Appl. Environ. Microbiol. : 6527 -6532.

    • Search Google Scholar
  • Kerényi, Z., Zeller, K., Hornok, L. and Leslie, J. F. (1999): Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex. Appl. Environ. Microbiol. 659, 4071–4076.

    Leslie J. F. , 'Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex ' (1999 ) 659 Appl. Environ. Microbiol. : 4071 -4076.

    • Search Google Scholar
  • Klittich, C. J. and Leslie, J. F. (1988): Nitrate reduction mutants of Fusarium moniliforme (Gibberella fujikuroi) . Genetics 118, 417–423.

    Leslie J. F. , 'Nitrate reduction mutants of Fusarium moniliforme (Gibberella fujikuroi) ' (1988 ) 118 Genetics : 417 -423.

    • Search Google Scholar
  • Kojima, K., Takano, Y., Yoshimi, A., Tanaka, C., Kikuchi., T. and Okuno, T. (2004): Fungicide activity through activation of a fungal signalling pathway. Mol. Microbiol. 53, 1785–1796.

    Okuno T. , 'Fungicide activity through activation of a fungal signalling pathway ' (2004 ) 53 Mol. Microbiol. : 1785 -1796.

    • Search Google Scholar
  • Kültz, D. (1998): Phylogenetic and functional classification of mitogen-and stress activated protein kinases. J. Mol. Evol. 46, 571–588.

    Kültz D. , 'Phylogenetic and functional classification of mitogen-and stress activated protein kinases ' (1998 ) 46 J. Mol. Evol. : 571 -588.

    • Search Google Scholar
  • Kwon, S., Dohlen, C. D. and Anderson, A. J. (2001): Gene sequence analysis of an opportunistic wheat pathogen, an isolate of Fusarium proliferatum . Can. J. Bot. 79, 1115–1121.

    Anderson A. J. , 'Gene sequence analysis of an opportunistic wheat pathogen, an isolate of Fusarium proliferatum ' (2001 ) 79 Can. J. Bot. : 1115 -1121.

    • Search Google Scholar
  • Leslie, J. F. and Summerell, B. (2006): Fusarium Laboratory Manual, Blackwell Publishing, Oxford.

    Summerell B. , '', in Fusarium Laboratory Manual , (2006 ) -.

  • Livak, K. J. and Schmittgen, T. D. (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2 -Delta C(T) method. Methods 25, 402–408.

    Schmittgen T. D. , 'Analysis of relative gene expression data using real-time quantitative PCR and the 2-Delta C(T) method ' (2001 ) 25 Methods : 402 -408.

    • Search Google Scholar
  • Ochiai, N., Tokai, T., Nishiuchi, T., Takahashi-Ando, N., Fujimura, M. and Kimura, M. (2007): Involvement of the osmosensor histidine kinase and osmotic stress-activated protein kinases in the regulation of secondary metabolism in Fusarium graminearum . Biochem. Biophys. Res. Commun. 363, 639–644.

    Kimura M. , 'Involvement of the osmosensor histidine kinase and osmotic stress-activated protein kinases in the regulation of secondary metabolism in Fusarium graminearum ' (2007 ) 363 Biochem. Biophys. Res. Commun. : 639 -644.

    • Search Google Scholar
  • Oláh, B., Jeney, A. and Hornok, L. (2006): Transient endophytic colonization of maize tissues by Fusarium proliferatum . Acta Phytopathol. Entomol. Hung. 41, 185–191.

    Hornok L. , 'Transient endophytic colonization of maize tissues by Fusarium proliferatum ' (2006 ) 41 Acta Phytopathol. Entomol. Hung. : 185 -191.

    • Search Google Scholar
  • Oren, L., Ezrati, S., Cohen, D. and Sharon, A. (2003): Early events in the Fusarium verticillioides -maize interaction characterized by using a green fluorescent protein-expressing transgenic isolate. Appl. Environ. Microbiol. 69, 1695–1701.

    Sharon A. , 'Early events in the Fusarium verticillioides-maize interaction characterized by using a green fluorescent protein-expressing transgenic isolate ' (2003 ) 69 Appl. Environ. Microbiol. : 1695 -1701.

    • Search Google Scholar
  • Park, S. M., Choi, E. S., Kim, M. J., Cha, B. J., Yang, M. S. and Kim, D. H. (2004): Characterization of HOG1 homologue, CpMK1 from Cryphonectria parasitica and evidence for hypovirus-mediated perturbation of its phosphorylation in response to hypertonic stress. Mol. Microbiol. 51, 1267–1277.

    Kim D. H. , 'Characterization of HOG1 homologue, CpMK1 from Cryphonectria parasitica and evidence for hypovirus-mediated perturbation of its phosphorylation in response to hypertonic stress ' (2004 ) 51 Mol. Microbiol. : 1267 -1277.

    • Search Google Scholar
  • Proctor, R. H., Hohn, T. M. and McCormick S. P. (1997): Restoration of wild-type virulence to Tri5 disruption mutants of Gibberella zeae via gene reversion and mutant complementation. Microbiology 143, 2583–2591.

    McCormick S. P. , 'Restoration of wild-type virulence to Tri5 disruption mutants of Gibberella zeae via gene reversion and mutant complementation ' (1997 ) 143 Microbiology : 2583 -2591.

    • Search Google Scholar
  • Punt, P. J., Oliver, R., Dingemanse, P., Pouwels, M. A. P. H. and van den Hondel, C. A. M. J. J. (1987): Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli . Gene 56, 117–124.

    Hondel C. A. M. J. J. , 'Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli ' (1987 ) 56 Gene : 117 -124.

    • Search Google Scholar
  • Ramakers, C., Ruijter, J. M., Deprez, R. H. and Moorman, A. F. (2003): Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339, 62–66.

    Moorman A. F. , 'Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data ' (2003 ) 339 Neurosci. Lett. : 62 -66.

    • Search Google Scholar
  • Sambrook, J. and Russell, R. W. (2001): Molecular cloning: A laboratory manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

    Russell R. W. , '', in Molecular cloning: A laboratory manual , (2001 ) -.

  • Schefe, J. H., Lehmann, K. E., Buschmann, I. R., Unger, T. and Funke-Kaiser, H. (2006): Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula. J. Mol. Med. 84, 901–910.

    Funke-Kaiser H. , 'Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula ' (2006 ) 84 J. Mol. Med. : 901 -910.

    • Search Google Scholar
  • Segmüller, N., Ellendorf, U., Tudzynski, B. and Tudzynski, P. (2007): BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea . Eukaryot. Cell 6, 211–221.

    Tudzynski P. , 'BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea ' (2007 ) 6 Eukaryot. Cell : 211 -221.

    • Search Google Scholar
  • Tanoue, T. and Nishida, E. (2003): Molecular recognition in the MAP kinase cascades. Cellul. Signal 15, 455–462.

    Nishida E. , 'Molecular recognition in the MAP kinase cascades ' (2003 ) 15 Cellul. Signal : 455 -462.

    • Search Google Scholar
  • Zhang, S. and Klessig, D. S. (2001): MAPK cascades in plant defense signaling. Trends Plant Sci. 6, 520–527.

    Klessig D. S. , 'MAPK cascades in plant defense signaling ' (2001 ) 6 Trends Plant Sci. : 520 -527.

    • Search Google Scholar
  • Zhang, Y., Lamm, R., Pillonel, C., Lam, S. and Xu, J. (2002): Osmoregulation and fungicide resistance: the Neurospora. crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue. Appl. Environ. Microbiol. 68, 532–538.

    Xu J. , 'Osmoregulation and fungicide resistance: the Neurospora. crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue ' (2002 ) 68 Appl. Environ. Microbiol. : 532 -538.

    • Search Google Scholar
  • Zwerger, K. and Hirt, H. (2001): Recent advances in plant MAP kinase signalling. Biol. Chem. 382, 1123–1131.

    Hirt H. , 'Recent advances in plant MAP kinase signalling ' (2001 ) 382 Biol. Chem. : 1123 -1131.

    • Search Google Scholar

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