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  • 1 Aligarh Muslim University Department of Botany Aligarh 202002 India
  • 2 Aligarh Muslim University Department of Agricultural Microbiology, Faculty of Agriculture Aligarh 202002 India
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Six potential isolates of Bacillus and Pseudomonas (out of 20 isolates screened under green house assay) were evaluated under pot and field conditions for the biocontrol of wilt disease complex of pigeonpea caused by Heterodera cajani, Meloidogyne spp. and Fusarium udum . Isolate Pa324 was found best for the biocontrol of wilt disease complex of pigeonpea followed by B18, Pf18, Pa70, Pa116 and B160 under pot condition. Isolate Pa324 caused greater reduction in multiplication of M. incognita and H. cajani and also reduced wilting caused by F. udum . Isolate Pa324 produced greater amount of siderophores followed by Pf18, Pa70 and Pa116. Isolate Pa324 showed greater hydrogen cyanide and indole acetic productions than other isolates. Moreover, seedling growth was higher when seeds were treated with Pf18 followed by Pa324, Pa116, B160 and B18. Isolate Pf18 caused a similar increase in seedling growth as was caused by Pa70. Under field condition too isolate Pa324 was best in reducing wilt disease complex followed by B18. Combined use of Pa324 with B18 provided better biocontrol of wilt disease complex than the use of either of them. Application of these isolates (Pa324 and B18) with Rhizobium sp. caused about 30% increase in yield under field condition and provided substantial protection against wilt disease complex of pigeonpea.

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