Acta Phytopathologica et Entomologica Hungarica
Volume/Issue:
Volume 44: Issue 2
Effect of Nucleopolyhedrovirus and Bacillus thuringiensis infection on the activity of insecticide detoxification enzymes in cotton bollworm, Helicoverpa armigera Hbn.
Authors: P. Duraimurugan, A. Regupathy 1 , and P. Shanmugam 1
View More View Less
  • 1 Tamil Nadu Agricultural University Department of Agricultural Entomology, Centre for Plant Protection Studies Coimbatore 641 003 India
  • 2 Indian Institute of Pulses Research Kanpur 208 024 Uttar Pradesh India
DOI:
https://doi.org/10.1556/aphyt.44.2009.2.11
Page Count:
345–352
Publication Date:
01 Dec 2009
Online Publication Date:
26 Nov 2009
Article Category:
Research Article
Restricted access

Purchase article

USD  $20.00

1 year subscription (Individual Only)

USD  $542.00
Purchase Online Subscription

  • Purchase article

    USD  $20.00

  • 1 year subscription (Individual Only)

    USD  $542.00

Enzyme assays were carried out to study the influence of Nucleopolyhedrovirus (HearNPV) and Bacillus thuringiensis subsp. kurstaki (Btk) infection on the activity levels of pesticide detoxification enzymes, viz. carboxyl esterase (CE) and mixed function oxidase (MFO) in Helicoverpa armigera . HearNPV at LC 50 reduced the MFO activity by 12.0–13.5% and CE activity by 14.88–15.25% at 96 hours after treatment. Btk at LC 50 reduced the MFO activity by 4.17–4.27% and CE activity by 5.77–6.55%. The joint application of HearNPV and cypermethrin at LC 50 +LC 50 resulted in suppression of MFO activity by 15.08–17.35% and CE activity by 16.39–17.30%. Combined treatment of Btk and cypermethrin resulted in suppression of MFO by 4.24–6.72 and CE by 8.42–8.97%, respectively.

  • Abdel-Aai, Y.A. I. and Soderbund, D.M. (1980): Pyrethroid hydrolysing esterases in southern armyworm larvae: Tissue distribution, kinetic properties and selective inhibition. Pestic. Biochem. Physiol. 14, 282–289.

    Soderbund D.M. , 'Pyrethroid hydrolysing esterases in southern armyworm larvae: Tissue distribution, kinetic properties and selective inhibition ' (1980 ) 14 Pestic. Biochem. Physiol. : 282 -289.

    • Search Google Scholar

  • Armes, N. J., Bond, G. S. and Cooter, R. J. (1992): The laboratory culture and development of Helicoverpa armigera . Natural Resource Institute Bulletin 57, Chatham, United Kingdom. 22. p.

  • Bradford, M. M. (1976): A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Ann. Biochem. 72, 248–254.

    Bradford M. M. , 'A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding ' (1976 ) 72 Ann. Biochem. : 248 -254.

    • Search Google Scholar

  • Bull, D. L. and Whitten, C. J. (1972): Factors influencing organophosphorus — insecticides resistance in tobacco budworms. J. Agric. Food Chem. 20, 561–564.

    Whitten C. J. , 'Factors influencing organophosphorus — insecticides resistance in tobacco budworms ' (1972 ) 20 J. Agric. Food Chem. : 561 -564.

    • Search Google Scholar

  • Devonshire, A. L. (1977): The properties of a carboxyl esterase from peach-potato aphid, Myzus persicae (Sulz.) and its role in conferring insecticide resistance. Biochem. J. 167, 675–683.

    Devonshire A. L. , 'The properties of a carboxyl esterase from peach-potato aphid, Myzus persicae (Sulz.) and its role in conferring insecticide resistance ' (1977 ) 167 Biochem. J. : 675 -683.

    • Search Google Scholar

  • Dittrich, V., Luetkemeier, N. and Voss, G. (1980): Organophosphorus-resistance in Spodoptera littoralis -inheritance, larval and imaginal expression and consequences for control. J. Econ. Entomol. 73, 356–362.

    Voss G. , 'Organophosphorus-resistance in Spodoptera littoralis-inheritance, larval and imaginal expression and consequences for control ' (1980 ) 73 J. Econ. Entomol. : 356 -362.

    • Search Google Scholar

  • Dowd, P. F. and Sparks, T. C. (1987): Comparisons of midgut, fat body and cuticular enzymes from Pseudoplusia includens (Walker) and Heliothis virescens (F.) responsible for the hydrolysis of permethrin and fenvalerate. Pestic. Biochem. Physiol. 27, 309–317.

    Sparks T. C. , 'Comparisons of midgut, fat body and cuticular enzymes from Pseudoplusia includens (Walker) and Heliothis virescens (F.) responsible for the hydrolysis of permethrin and fenvalerate ' (1987 ) 27 Pestic. Biochem. Physiol. : 309 -317.

    • Search Google Scholar

  • Duraimurugan, P. and Regupathy, A. (2008): Synergistic effects of nucleopolyhedrovirus with synthetic pyrethroids against Helicoverpa armigera (Hübner). Pestic. Res. J. 20, 83–86.

    Regupathy A. , 'Synergistic effects of nucleopolyhedrovirus with synthetic pyrethroids against Helicoverpa armigera (Hübner) ' (2008 ) 20 Pestic. Res. J. : 83 -86.

    • Search Google Scholar

  • Duraimurugan, P. and Regupathy, A. (2004): Biological suppression of synthetic pyrethroids resistance in Helicoverpa armigera (Hubner) by Bacillus thuringiensis subsp. kurstaki . International Symposium, Strategies for Sustainable Cotton Production — A Global Vision. Dharward, India, Nov. 23–25, pp. 216–218.

  • Gomez, K. A. and Gomez, A. A. (1976): Statistical Procedures for Agricultural Research with Special Emphasis on Rice. International Rice Research Institute, Los Banos, Philippines. 368 p.

    Gomez A. A. , '', in Statistical Procedures for Agricultural Research with Special Emphasis on Rice , (1976 ) -.

  • Hansen, L. G. and Hodgson, E. (1971). Biochemical characteristics of insect microsomes N and O-demethylation. Biochem. Pharmacol. 20, 1569–1578.

    Hodgson E. , 'Biochemical characteristics of insect microsomes N and O-demethylation ' (1971 ) 20 Biochem. Pharmacol. : 1569 -1578.

    • Search Google Scholar

  • Hosny, A. H. and Abassy, M.A. (1977): Correlation between lipid content, acetylcholinesterase activity and toxicity of some new insecticides to Egyptian cotton leaf worm Spodoptera littoralis . Faculty of Agriculture, Tant. University, Egypt.

    Abassy M.A. , '', in Correlation between lipid content, acetylcholinesterase activity and toxicity of some new insecticides to Egyptian cotton leaf worm Spodoptera littoralis , (1977 ) -.

  • Kranthi, K. R. (2005): Insecticide Resistance — Monitoring, Mechanisms and Management Manual. Central Institute for Cotton Research, P.B. No. 2, Shankarnagar, Nagpur 440 010, India. pp. 49–87.

    Kranthi K. R. , '', in Insecticide Resistance — Monitoring, Mechanisms and Management Manual , (2005 ) -.

  • Leethial, R. P. and Regupathy, A. (2004): Biological suppression of synthetic pyrethroids resistance in Helicoverpa armigera (Hubner) by nuclear polyhedrosis viruses (HaNPV). International Plant Protection Congress, Beijing, China, July 6–11, 254. p.

  • Pawar, C. S. (2004): Present status and future needs for the management of Helicoverpa . International Workshop on Entomopathogenic fungi — A valuable alternative to fight against insect pests, Pune, India, Sept. 16–18, pp. 21–28.

  • Phokela, A., Srivastava, M., Singh, S. P., Singh, A. K. and Mehrotra, K. N. (1997): Response of Helicoverpa armigera (Hubner) population in Delhi to a pyrethroid and organophosphates. Pestic. Res. J. 9, 101–104.

    Mehrotra K. N. , 'Response of Helicoverpa armigera (Hubner) population in Delhi to a pyrethroid and organophosphates ' (1997 ) 9 Pestic. Res. J. : 101 -104.

    • Search Google Scholar

  • Regupathy, A., Kranthi, K., Singh, J., Iqabal, A., Wu, Y. and Russell, D.A. (2004): Patterns and Magnitude of insectivore resistance levels in India, Pakistan and China. World Cotton Conference III, March 9–13, 2004, Cape Town, South Africa. pp. 1215–1229.

  • Salama, H. S. and Sharaby, A. (1985): Histopathological changes in Heliothis armigera infected with Bacillus thuringiensis as detected by electron microscopy. Insect Sci. Applic. 6, 503–511.

    Sharaby A. , 'Histopathological changes in Heliothis armigera infected with Bacillus thuringiensis as detected by electron microscopy ' (1985 ) 6 Insect Sci. Applic. : 503 -511.

    • Search Google Scholar

  • Song, W. C., Lian, S. X. and Yu, Z. G. (2000): Synergism of Helicoverpa armigera nucleopolyhedrovirus in combination with chemical insecticides and lecithin. Acta Entomologica Sinica, 43, 346–355.

    Yu Z. G. , 'Synergism of Helicoverpa armigera nucleopolyhedrovirus in combination with chemical insecticides and lecithin ' (2000 ) 43 Acta Entomologica Sinica : 346 -355.

    • Search Google Scholar

  • Tamilselvi, S. and Regupathy, A. (2002): Synergistic suppression of pyrethroid resistance in Helicoverpa armigera (Hubner). In: B. Sarath Babu, K. S. Varaprasad, K. Anitha, R. D. V. J. Prasada Rao and P. S. Chandurkar (eds): Resources Management in Plant Protection, Plant Protection Association of India, Hyderabad, India. pp. 58–61.

    Regupathy A. , '', in Resources Management in Plant Protection , (2002 ) -.

  • Thilagam, P. and Kennedy, J. S. (2004): Qualitative and quantitative assessment of synergism between Bacillus thuringiensis var. kurstaki and insecticides. National Symposium, Green Pesticides for Insect PestManagement, Chennai, India, Feb. 5–6, 69. p.

  • Wu, G., Minsheng, Y., Shixi, Z. and Shu Ren, J. (2001): Synergism of Bacillus thuringiensis pretreatment to organophosphate and carbamate insecticide in Plutella xylostella . Acta Entomologica Sinica, 44, 454–461.

    Shu Ren J. , 'Synergism of Bacillus thuringiensis pretreatment to organophosphate and carbamate insecticide in Plutella xylostella ' (2001 ) 44 Acta Entomologica Sinica : 454 -461.

    • Search Google Scholar
Cover Acta Phytopathologica et Entomologica Hungarica
Acta Phytopathologica et Entomologica Hungarica
Print ISSN:
0238-1249
Online ISSN:
1588-2691
Keywords:
Helicoverpa armigera ; HearNPV; Btk; detoxification enzymes

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 1 0 0
Sep 2020 2 0 0
Oct 2020 1 0 0
Nov 2020 1 0 0
Dec 2020 0 0 0
Jan 2021 1 0 0
Feb 2021 0 0 0