Acta Phytopathologica et Entomologica Hungarica
Volume/Issue:
Volume 45: Issue 1
Sequence comparison of the HC-Pro and CP proteins of two Potato virus Y strains differing in aphid transmission and systemic movement
Authors: A. Almási 1 , I. Tóbiás 1 , I. Manoussopoulos 2 , Zs. Basky 1 , and L. Palkovics 3
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  • 1 Hungarian Academy of Sciences Plant Protection Institute H-1022 Budapest Herman O. út 15 Hungary
  • 2 NEO and Amerikis Plant Protection Institute of Patras, Laboratory of Virology 26004 Patras Greece
  • 3 Corvinus University of Budapest Department of Plant Pathology, Faculty of Horticultural Science H-1118 Budapest Villányi út 29-43 Hungary
DOI:
https://doi.org/10.1556/aphyt.45.2010.1.1
Page Count:
1–11
Publication Date:
01 Jun 2010
Online Publication Date:
10 Jun 2010
Article Category:
Research Article
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Nucleotide and amino acid sequences of the helper component protease (HC-Pro) and the coat protein (CP) of two Hungarian Potato virus Y (PVY) isolates, differing in aphid transmissibility were determined. Isolate PVY-5 belongs to the common “O” strain (PVY O ), whereas isolates PVY-98 and PVY-111 belong to the “N” (PVY N ) and the PVY-NTN and PVY-H to the “NTN” (PVY NTN ) strains, respectively. The PVY-5 isolate varied significantly from the others in aphid transmission and in the ability to systemically infect potato plants. To elucidate whether these differences were due to mutations affecting known functional motifs, the corresponding cistrons of the two proteins were sequenced and aligned. Our analysis showed that none of the well-known motifs, responsible for aphid transmission in the two proteins had been affected. However, the defective isolate had two natural mutations in the HC-Pro in the vicinity of the PTK motif, and a number of mutations in the CP, distributed both in the N-terminus and the central region. As these two proteins are the only known viral participants in the aphid transmission mechanism, it is likely that some of the observed mutations might be involved in this process. Thus, our results indicate that other, previously unidentified sequences or factors may influence virus-vector interactions and transmission of PVY.

  • Atreya, C. D., Raccah, B. and Pirone, T. P. (1990): A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178, 161–165.

    Pirone T. P. , 'A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus ' (1990 ) 178 Virology : 161 -165.

    • Search Google Scholar

  • Atreya, C. D. and Pirone, T. P. (1993): Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proc. Natl. Acad. Sci. USA 90, 11919–11923.

    Pirone T. P. , 'Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility ' (1993 ) 90 Proc. Natl. Acad. Sci. USA : 11919 -11923.

    • Search Google Scholar

  • Basky, Zs. and Almási, A. (2005): Differences in aphid transmissibility and translocation between PVY N and PVY O isolates. J. Pest Sci. 78, 67–78.

    Almási A. , 'Differences in aphid transmissibility and translocation between PVYN and PVYO isolates ' (2005 ) 78 J. Pest Sci. : 67 -78.

    • Search Google Scholar

  • Blanc, S., López-Moya, J. J., Wang, R. Y., Garcia Lampasona, S., Thornbury, D. W. and Pirone, T. P. (1997): A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus. Virology 231, 141–147.

    Pirone T. P. , 'A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus ' (1997 ) 231 Virology : 141 -147.

    • Search Google Scholar

  • Beczner, L., Horváth, J., Romhányi, I. and Förster, H. (1984): Studies on the etiology of tuber necrotic ringspot disease in potato. Potato Res. 27, 339–352.

    Förster H. , 'Studies on the etiology of tuber necrotic ringspot disease in potato ' (1984 ) 27 Potato Res. : 339 -352.

    • Search Google Scholar

  • Deborré, G., Maiss, E. and Jelkmann, W. (1995): Biological and molecular biological investigations of several Plum pox virus (PPV) isolates. Acta Horticulturae 386, 253–262.

    Jelkmann W. , 'Biological and molecular biological investigations of several Plum pox virus (PPV) isolates ' (1995 ) 386 Acta Horticulturae : 253 -262.

    • Search Google Scholar

  • Govier, D. A. and Kassanis, B. (1974): A virus induced component of plant sap needed when aphids acquire potato virus Y from purified preparations. Virology 61, 420–426.

    Kassanis B. , 'A virus induced component of plant sap needed when aphids acquire potato virus Y from purified preparations ' (1974 ) 61 Virology : 420 -426.

    • Search Google Scholar

  • Harrison, B. D. and Robinson, D. J. (1988): Molecular variation in vector-borne plant viruses: Epidemiological significance. Philos. Trans. R. Soc. London Biol. 321, 447–462.

    Robinson D. J. , 'Molecular variation in vector-borne plant viruses: Epidemiological significance ' (1988 ) 321 Philos. Trans. R. Soc. London Biol. : 447 -462.

    • Search Google Scholar

  • Kang, S. H., Lim, W. S., Hwang, S. H., Park, J.W., Choi, H. S. and Kim, K. H. (2006): Importance of the C-terminal domain of soybean mosaic virus coat protein for subunit interactions. J. Gen. Virol. 87, 225–229.

    Kim K. H. , 'Importance of the C-terminal domain of soybean mosaic virus coat protein for subunit interactions ' (2006 ) 87 J. Gen. Virol. : 225 -229.

    • Search Google Scholar

  • Kassanis, B. (1961): The transmission of potato aucuba mosaic virus by aphids from plants also infected by potato virus A and Y. Virology 13, 93–97.

    Kassanis B. , 'The transmission of potato aucuba mosaic virus by aphids from plants also infected by potato virus A and Y ' (1961 ) 13 Virology : 93 -97.

    • Search Google Scholar

  • Kimalov, B., Gal-On, A., Stav, R., Belausov, E. and Arazi, T. (2004): Maintenance of coat protein N-terminal net charge and not primary sequence is essential for zucchini yellow mosaic virus systemic infectivity. J. Gen. Virol. 85, 3421–3430.

    Arazi T. , 'Maintenance of coat protein N-terminal net charge and not primary sequence is essential for zucchini yellow mosaic virus systemic infectivity ' (2004 ) 85 J. Gen. Virol. : 3421 -3430.

    • Search Google Scholar

  • Llave, C., Martinez, B., Diaz-Ruiz, J. R. and Lopez-Abella, D. (2002): Amino acid substitutions within the Cysrich domain of the tobacco etch potyvirus HC-Pro result in loss of transmissibility by aphids. Arch. Virol. 147, 2365–2375.

    Lopez-Abella D. , 'Amino acid substitutions within the Cysrich domain of the tobacco etch potyvirus HC-Pro result in loss of transmissibility by aphids ' (2002 ) 147 Arch. Virol. : 2365 -2375.

    • Search Google Scholar

  • López-Moya, J. J., Wang, R.Y. and Pirone, T. P. (1999): Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. J. Gen. Virol. 80, 3281–3288.

    Pirone T. P. , 'Context of the coat protein DAG motif affects potyvirus transmissibility by aphids ' (1999 ) 80 J. Gen. Virol. : 3281 -3288.

    • Search Google Scholar

  • Maia, I. G., Haenni, A.-L. and Bernardi, F. (1996): Potyviral HC-Pro: A multifunctional protein. J. Gen.Virol. 77, 1335–1341.

    Bernardi F. , 'Potyviral HC-Pro: A multifunctional protein ' (1996 ) 77 J. Gen.Virol. : 1335 -1341.

    • Search Google Scholar

  • Manoussopoulos, I. N., Maiss, E. and Tsagris, M. (2000): Native electrophoresis and Western blot analysis (NEWeB): A method for characterization of different forms of potyvirus particles and similar nucleoprotein complexes in extracts of infected plant tissues. J. Gen. Virol. 81, 2295–2298.

    Tsagris M. , 'Native electrophoresis and Western blot analysis (NEWeB): A method for characterization of different forms of potyvirus particles and similar nucleoprotein complexes in extracts of infected plant tissues ' (2000 ) 81 J. Gen. Virol. : 2295 -2298.

    • Search Google Scholar

  • Nakashima, H., Sako, N. and Hori, K. (1993): Nucleotide sequences of the helper component-proteinase genes of aphid transmissible and non-transmissible isolates of turnip mosaic virus. Arch. Virol. 131, 17–27.

    Hori K. , 'Nucleotide sequences of the helper component-proteinase genes of aphid transmissible and non-transmissible isolates of turnip mosaic virus ' (1993 ) 131 Arch. Virol. : 17 -27.

    • Search Google Scholar

  • Nie, X. and Singh, R. (2002): A new approach for the simultaneous differentiation of biological and geographical strains of Potato virus Y by uniplex and multiplex RT-PCR. J. Virol. Meth. 104, 41–54.

    Singh R. , 'A new approach for the simultaneous differentiation of biological and geographical strains of Potato virus Y by uniplex and multiplex RT-PCR ' (2002 ) 104 J. Virol. Meth. : 41 -54.

    • Search Google Scholar

  • Oh, C. S. and Carrington, J. C. (1989): Identification of essential residues in potyvirus proteinase HC-Pro by site-directed mutagenesis. Virology 173, 692–699.

    Carrington J. C. , 'Identification of essential residues in potyvirus proteinase HC-Pro by site-directed mutagenesis ' (1989 ) 173 Virology : 692 -699.

    • Search Google Scholar

  • Pirone, T. P. (1991): Viral genes and gene products that determine insect transmissibility. Seminars in Virology 2, 81–87.

    Pirone T. P. , 'Viral genes and gene products that determine insect transmissibility ' (1991 ) 2 Seminars in Virology : 81 -87.

    • Search Google Scholar

  • Pirone, T. P. and Blanc, S. (1996): Helper-dependent vector transmission of plant viruses. Ann. Rev. Phytopathol. 34, 227–247.

    Blanc S. , 'Helper-dependent vector transmission of plant viruses ' (1996 ) 34 Ann. Rev. Phytopathol. : 227 -247.

    • Search Google Scholar

  • Sambrook, J., Fritsch, E. F. and Maniatis, T.A. (1989): Molecular Cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Maniatis T.A. , '', in Molecular Cloning: A Laboratory Manual , (1989 ) -.

  • Shukla, D. D., Ward, C.W. and Brunt, A. A. (1994): The Potyviridae. Cambridge University Press, Cambridge.

    Brunt A. A. , '', in The Potyviridae , (1994 ) -.

  • Singh, R. P., McLaren, D. L., Nie, X. and Singh, M. (2003): Possible escape of a recombinant isolate of Potato virus Y by serological indexing and methods of its detection. Plant Disease, 87, 679–685.

    Singh M. , 'Possible escape of a recombinant isolate of Potato virus Y by serological indexing and methods of its detection ' (2003 ) 87 Plant Disease : 679 -685.

    • Search Google Scholar

  • Swenson, K. G., Sohi, S. S. and Welton, R. E. (1964): Loss of transmissibility by aphids of bean yellow mosaic virus. Ann. Entomol. Soc. Am. 57, 378–382.

    Welton R. E. , 'Loss of transmissibility by aphids of bean yellow mosaic virus ' (1964 ) 57 Ann. Entomol. Soc. Am. : 378 -382.

    • Search Google Scholar

  • Thole V., Dalmay, T., Burgyán, J. and Balázs, E. (1993): Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene 123, 149–156.

    Balázs E. , 'Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA ' (1993 ) 123 Gene : 149 -156.

    • Search Google Scholar

  • Urcuqui-Inchima, S., Haenni, A. and Bernardi, F. (2001): Potyvirus proteins: A wealth of functions. Virus Res. 74, 157–175.

    Bernardi F. , 'Potyvirus proteins: A wealth of functions ' (2001 ) 74 Virus Res. : 157 -175.

  • Wang, R.Y., Powell, G., Hardie, J. and Pirone, T. P. (1998): Role of the helper component in vector-specific transmission of potyviruses. J. Gen. Virology 79, 1519–1524.

    Pirone T. P. , 'Role of the helper component in vector-specific transmission of potyviruses ' (1998 ) 79 J. Gen. Virology : 1519 -1524.

    • Search Google Scholar

  • White, J. L. and Kaper, J. M. (1989): A simple method for detection of viral satellite RNA in small tissue samples. J. Virological Methods 23, 83–94.

    Kaper J. M. , 'A simple method for detection of viral satellite RNA in small tissue samples ' (1989 ) 23 J. Virological Methods : 83 -94.

    • Search Google Scholar
Cover Acta Phytopathologica et Entomologica Hungarica
Acta Phytopathologica et Entomologica Hungarica
Print ISSN:
0238-1249
Online ISSN:
1588-2691
Keywords:
helper component; coat protein; aphid transmission; PVY; DAG motif; KITC motif; PTK motif; nucleotide and amino acid sequence

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