Acta Phytopathologica et Entomologica Hungarica
Volume/Issue:
Volume 46: Issue 1
Inhibition of virus replication and symptom expression by reactive oxygen species in tobacco infected with Tobacco mosaic virus
Authors: R. Bacsó 1 , Y. Hafez 1 , Z. Király 1 , and L. Király 2
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  • 1 Hungarian Academy of Sciences Department of Pathophysiology, Plant Protection Institute H-1525 Budapest P.O. Box 102 Hungary
  • 2 Hungarian Academy of Sciences Department of Biotechnology, Plant Protection Institute H-1525 Budapest P.O. Box 102 Hungary
DOI:
https://doi.org/10.1556/aphyt.46.2011.1.1
Page Count:
1–10
Publication Date:
01 Jun 2011
Online Publication Date:
26 May 2011
Article Category:
Research Article
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In spite of the enormous information from research on genetics of plant disease resistance, the question still remains unresolved: what is directly inhibiting or killing pathogens and suppressing symptoms in resistant plants? This is particularly true for resistance to viral infections. Here we show that externally applied reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) or ROS-producing (O 2·− [superoxide] and H2O2) chemical systems infiltrated into tobacco leaves 2 hours after inoculation suppress replication of Tobacco mosaicvirus (TMV) in the susceptible Samsun (nn) cultivar. This was determined by a biological and a real-time PCR method. Infiltration of leaves of the resistant Xanthi (NN) cultivar with the ROS-producing chemicals and H2O2 significantly suppressed local necrotic lesions (i.e. the hypersensitive response) after inoculation of tobacco leaves with TMV. Accordingly, an early accumulation or external application of ROS, such as O 2·− and H2O2, in tobacco may contribute to the development of resistance to TMV infection.

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Cover Acta Phytopathologica et Entomologica Hungarica
Acta Phytopathologica et Entomologica Hungarica
Print ISSN:
0238-1249
Online ISSN:
1588-2691
Keywords:
Tobacco mosaic virus ; reactive oxygen species; plant virus resistance

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