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  • 1 Hungarian Academy of Sciences Plant Protection Institute, Centre for Agricultural Research H-1525 Budapest P.O.Box 102 Hungary
  • 2 Yüzüncü Yıl Üniversity Agricultural Faculty, Plant Protection Department 65080 Kampüs, Van Turkey
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Planococcus citri (Risso, 1813) and Planococcus ficus (Signoret, 1875) (Hemiptera: Pseudococcidae) are important polyphagous pests species. Their high degree of morphological similarity in male and larval stage makes them difficult to distinguish. The aim of the study was to find a simple and fast PCR-based method to separate these two mealybug species. Thanks to the use of a short DNA extraction method and species-specific primer pairs, P. citri and P. ficus can be distinguished at any developmental stages within three hours.

  • Ben-Dov, Y., Mıller, D. R. and Gibson, G. A. P. (2010): ScaleNet, A database of scale insects of the world: http://www.Sel.Barc.Usda.Gov.ScalenetScalenet

  • Cox, J. (1989): The mealybug genus Planococcus (Homoptera: Pseudococcidae). Bull. Br. Mus. Nat. Hist. Entomol. 58, 1–78.

    Cox J. , 'The mealybug genus Planococcus (Homoptera: Pseudococcidae) ' (1989 ) 58 Bull. Br. Mus. Nat. Hist. Entomol. : 1 -78.

    • Search Google Scholar
  • Demontis, M. A., Ortu, S., Cocco, A., Lentini, A., and Migheli, Q. (2007): Diagnostic markers for Planococcus ficus (Signoret) and Planococcus citri (Risso) by random amplification of polymorphic DNA-polymerase chain reaction and species-specific mitochondrial DNA primers. J. Appl. Entomol. 131, 59–64.

    Migheli Q. , 'Diagnostic markers for Planococcus ficus (Signoret) and Planococcus citri (Risso) by random amplification of polymorphic DNA-polymerase chain reaction and species-specific mitochondrial DNA primers ' (2007 ) 131 J. Appl. Entomol. : 59 -64.

    • Search Google Scholar
  • Gullan, P. J., Kaydan, M. B. and Hardy, N. B. (2010): Molecular phylogeny and species recognition in the mealybug genus Ferrisia Fullaway (Hemiptera: Pseudococcidae). Syst. Entomol. 35, 1–11.

    Hardy N. B. , 'Molecular phylogeny and species recognition in the mealybug genus Ferrisia Fullaway (Hemiptera: Pseudococcidae) ' (2010 ) 35 Syst. Entomol. : 1 -11.

    • Search Google Scholar
  • Hardy, N. B., Gullan, P. J. and Hodgson, C. J., (2008): A subfamily level classification of mealybugs (Hemiptera: Pseudococcidae) based on integrated molecular and morphological data. Syst. Entomol. 33, 51–71.

    Hodgson C. J. , 'A subfamily level classification of mealybugs (Hemiptera: Pseudococcidae) based on integrated molecular and morphological data ' (2008 ) 33 Syst. Entomol. : 51 -71.

    • Search Google Scholar
  • Kim, H. and Lee, S. (2008): Molecular systematics of the genus Megoura (Hemiptera: Aphididae) using mitochondrial and nuclear DNA sequences. Molecules and Cells, 25, 510–522.

    Lee S. , 'Molecular systematics of the genus Megoura (Hemiptera: Aphididae) using mitochondrial and nuclear DNA sequences ' (2008 ) 25 Molecules and Cells : 510 -522.

    • Search Google Scholar
  • Malausa, T., Fenis, A., Warot, S., Germain, J. F., Ris, N., Prado, E., Botton, M., Vanlerbetghe-Masutti, F., Sforza, R., Cruaud, C., Couloux, A. and Kreiter, P. (2010): DNA markers to disentangle comlexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae). J. Appl. Entomol. 135, 142–155.

    Kreiter P. , 'DNA markers to disentangle comlexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae) ' (2010 ) 135 J. Appl. Entomol. : 142 -155.

    • Search Google Scholar
  • Rung, A., Scheffer, S. J., Evans, G. and Miller, D. (2008): Molecular identification of two closely related species of mealybugs of the genus Planococcus (Homoptera: Pseudococcidae). Ann. Entomol. Soc. Am. 101, 525–532.

    Miller D. , 'Molecular identification of two closely related species of mealybugs of the genus Planococcus (Homoptera: Pseudococcidae) ' (2008 ) 101 Ann. Entomol. Soc. Am. : 525 -532.

    • Search Google Scholar
  • Tóbiás, I., Kozár, F., Kaydan, B. M. and Fetykó, K. (2010): Use of molecular tools for the identification of males of some scale insects (Hemiptera: Coccidea), in pheromone traps used for monitoring and comparison with females. J. Ent. Acar. Res. Ser. II, 42, 171–181.

    Fetykó K. , 'Use of molecular tools for the identification of males of some scale insects (Hemiptera: Coccidea), in pheromone traps used for monitoring and comparison with females ' (2010 ) 42 J. Ent. Acar. Res. Ser. II : 171 -181.

    • Search Google Scholar
  • Tóbiás, I., Kozár, F. and Kaydan, B. M. (2011): Molekuláris módszerek alkalmazása néhány pajzstetűfaj azonosítására. (Use of molecular tools for the identification of some mealybug species). Növényvédelem 47, 273–278

    Kaydan B. M. , 'Molekuláris módszerek alkalmazása néhány pajzstetűfaj azonosítására ' (2011 ) 47 Növényvédelem : 273 -278.

    • Search Google Scholar