View More View Less
  • 1 Indian Agricultural Research Institute Division of Fruits and Horticultural Technology New Delhi India
  • 2 Indian Agricultural Research Institute National Research Centre on Plant Biotechnology New Delhi India
  • 3 Indian Agricultural Research Institute Division of Nuclear Research Laboratory New Delhi India
Restricted access

Purchase article

USD  $20.00

1 year subscription (Individual Only)

USD  $542.00

Mango malformation is one of the most important diseases limiting its cultivation. However, disease resistance is known in some mango cultivars and is a desirable trait that can be utilized for developing mango varieties resistant to malformation. Resistance genes cloned from different plant species have revealed several similarities in DNA sequence and structural motifs. This provides the possibility of isolating resistance genes by polymerase chain reaction (PCR) with degenerate oligonucleotide primers designed from highly conserved regions of the nucleotide binding site (NBS). In the present study, we used eight combinations of oligonucleotide primers designed on the basis of P-loop and hydrophobic domains of conserved NBS-leucine rich repeat (LRR) protein sequences for amplifying resistance gene analogues (RGAs) in eight mango cultivars and hybrids showing a variable degree of resistance to mango malformation disease. A single band of about 500 bp in all mango cultivars was obtained from the s2+as2 primer combination. RGAs isolated from mango showed 73% similarity with RGAs in databases. It confirms that RGAs were actually isolated from mango. The obtained sequence can be used for isolating full length R-genes.

  • Aarts, M. G. M., te Lintel Hekkert, B., Holub, E. B., Beynon, J. L., Stiekema, W. J. and Pereira, A. (1998): Identification of R-gene homologous DNA fragments genetically linked to resistance loci in Arabidopsis thaliana. Mol. Pl. Microbe Interact. 11, 252–258.

    Pereira A. , 'Identification of R-gene homologous DNA fragments genetically linked to resistance loci in Arabidopsis thaliana ' (1998 ) 11 Mol. Pl. Microbe Interact. : 252 -258.

    • Search Google Scholar
  • Baldi, P., Patocchi, A., Zini, E., Toller, C., Velaso, R. and Komjanc, M. (2004): Cloning and linkage mapping of resistance gene homologues in apple. Theor. Appl. Genet. 109, 231–239.

    Komjanc M. , 'Cloning and linkage mapping of resistance gene homologues in apple ' (2004 ) 109 Theor. Appl. Genet. : 231 -239.

    • Search Google Scholar
  • Belkhadir, Y., Subramaniam, R. and Dangl, J. L. (2004): Plant disease resistance protein signalling: NBS-LRR proteins and their partners. Current Opinion Pl. Bio. 7, 391–399.

    Dangl J. L. , 'Plant disease resistance protein signalling: NBS-LRR proteins and their partners ' (2004 ) 7 Current Opinion Pl. Bio. : 391 -399.

    • Search Google Scholar
  • Britz, H., Steenkamp, E. T., Coutinho, T. A., Wingfield, B. D., Marasas, W. F. O. and Wingfield, M. J. (2002): Two new species of Fusarium section Liseola associated with mango malformation. Mycologia. 94, 722–730.

    Wingfield M. J. , 'Two new species of Fusarium section Liseola associated with mango malformation ' (2002 ) 94 Mycologia. : 722 -730.

    • Search Google Scholar
  • Collins, N. C., Webb, C. A., Seah, S., Ellis, J. G., Hulbert, S. H. and Pryor, A. (1998): The isolation and mapping of disease resistance gene analogs in maize. Mol. Pl. Microbe Interact. 11, 968–978.

    Pryor A. , 'The isolation and mapping of disease resistance gene analogs in maize ' (1998 ) 11 Mol. Pl. Microbe Interact. : 968 -978.

    • Search Google Scholar
  • Dangl, J. L. and Jones, J. D. G. (2001): Plant pathogens and integrated defense responses to infection. Nature 411, 826–833.

    Jones J. D. G. , 'Plant pathogens and integrated defense responses to infection ' (2001 ) 411 Nature : 826 -833.

    • Search Google Scholar
  • Dilbirligi, M., Erayman, M., Sandhu, D., Sidhu, D. and Gill, K. S. (2004): Identification of wheat chromosomal regions containing expressed resistance genes. Genetics 166, 461–481.

    Gill K. S. , 'Identification of wheat chromosomal regions containing expressed resistance genes ' (2004 ) 166 Genetics : 461 -481.

    • Search Google Scholar
  • Dixon, M. S., Jones, D. A, Keddle, J. S., Thomas, C. M., Harrison, K. and Jones, J. D. G. (1996): The tomato cf-2 disease resistance locus comprises two functional genes coding leucine-rich repeat proteins. Cell 84, 451–459.

    Jones J. D. G. , 'The tomato cf-2 disease resistance locus comprises two functional genes coding leucine-rich repeat proteins ' (1996 ) 84 Cell : 451 -459.

    • Search Google Scholar
  • Donald, T. M., Pellerone, F., Adam-Blondon, A. F., Bouquet, A., Thomas, M. R. and Dry, I. B. (2002): Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine. Theor. Appl. Genet. 104, 610–618.

    Dry I. B. , 'Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine ' (2002 ) 104 Theor. Appl. Genet. : 610 -618.

    • Search Google Scholar
  • Freeman, S., Maimon, M. and Pinkas, Y. (1999): Use of GUS transformants of Fusarium subglutinans for determining etiology of mango malformation disease. Phytopathology 89, 456–461.

    Pinkas Y. , 'Use of GUS transformants of Fusarium subglutinans for determining etiology of mango malformation disease ' (1999 ) 89 Phytopathology : 456 -461.

    • Search Google Scholar
  • Freeman, S., Klein-Gueta, D., Korolev, N. and Sztejnberg, A. (2004): Epidemiology and survival of Fusarium mangiferae, the causal agent of mango malformation disease. Acta Hort. 645, 487–491.

    Sztejnberg A. , 'Epidemiology and survival of Fusarium mangiferae, the causal agent of mango malformation disease ' (2004 ) 645 Acta Hort. : 487 -491.

    • Search Google Scholar
  • Hammond-Kosack, K. E. and Jones, J. D. G. (1997): Plant disease resistance genes. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 575–607.

    Jones J. D. G. , 'Plant disease resistance genes ' (1997 ) 48 Annu. Rev. Plant Physiol. Plant Mol. Biol. : 575 -607.

    • Search Google Scholar
  • Hulbert, S. H., Webb, C. A., Smith, S. M. and Sun, Q. (2001): Resistance gene complexes: evolution and utilization. Annu. Rev. Phytopathol. 39, 285–312.

    Sun Q. , 'Resistance gene complexes: evolution and utilization ' (2001 ) 39 Annu. Rev. Phytopathol. : 285 -312.

    • Search Google Scholar
  • Johal, G. S. and Briggs, S. P. (1992): Reductase activity encoded by HM1 disease resistance gene in maize. Science 258, 985–987.

    Briggs S. P. , 'Reductase activity encoded by HM1 disease resistance gene in maize ' (1992 ) 258 Science : 985 -987.

    • Search Google Scholar
  • Kanazin, V., Marek, L. F. and Shoemaker, R. C. (1996): Resistance gene analogs are conserved and clustered in soybean. Proc. Natl Acad. Sci. USA, 93, 11746–11750.

    Shoemaker R. C. , 'Resistance gene analogs are conserved and clustered in soybean ' (1996 ) 93 Proc. Natl Acad. Sci. USA : 11746 -11750.

    • Search Google Scholar
  • Kumar, J., Singh, U. S. and Beniwal, S. P. S. (1993): Mango malformation: one hundred years of research. Annu. Rev. Phytopathol. 31, 217–232.

    Beniwal S. P. S. , 'Mango malformation: one hundred years of research ' (1993 ) 31 Annu. Rev. Phytopathol. : 217 -232.

    • Search Google Scholar
  • Kumar, S. P., Dalal, V., Singh, N. K. and Sharma, T. R. (2007): Cloning and in silico mapping of resistance gene analogues isolated from rice lines containing known genes for blast resistance. J. Phytopathol. 155, 273–280.

    Sharma T. R. , 'Cloning and in silico mapping of resistance gene analogues isolated from rice lines containing known genes for blast resistance ' (2007 ) 155 J. Phytopathol. : 273 -280.

    • Search Google Scholar
  • Kvas, M., Steenkamp, E. T., Al Adawi, A. O., Deadman M. L., Al Jahwari, A. A., Marasas, W. F. O., Wingfield, B. D., Ploetz, R. C. and Wingfield, M. J. (2008): Fusarium mangiferae associated with mango malformation in the Sultanate of Oman. Eur. J. Plant Pathol. 121, 195–199.

    Wingfield M. J. , 'Fusarium mangiferae associated with mango malformation in the Sultanate of Oman ' (2008 ) 121 Eur. J. Plant Pathol. : 195 -199.

    • Search Google Scholar
  • Leister, D., Ballova, A., Salamini, F. and Gebhardt, C. (1996): A PCR-based approach for isolating pathogen resistance genes from potato with a potential wide application in plants. Nat. Genet. 14, 421–429.

    Gebhardt C. , 'A PCR-based approach for isolating pathogen resistance genes from potato with a potential wide application in plants ' (1996 ) 14 Nat. Genet. : 421 -429.

    • Search Google Scholar
  • Leister, D., Kurth, J., Laurie, D. A., Yano, M., Sasaki, T., Devos, K., Graner, A. and Schulze-Lefert, P. (1998): Rapid reorganization of resistance gene homologues in cereal genome. Prot. Natl Acad. Sci. USA. 95, 370–375.

    Schulze-Lefert P. , 'Rapid reorganization of resistance gene homologues in cereal genome ' (1998 ) 95 Prot. Natl Acad. Sci. USA. : 370 -375.

    • Search Google Scholar
  • Mago, R., Nair, S. and Mohan, M. (1999): Resistance gene analogues from rice. Theor. Appl. Genet. 99, 50–57.

    Mohan M. , 'Resistance gene analogues from rice ' (1999 ) 99 Theor. Appl. Genet. : 50 -57.

  • Martin, G. B., Brommonshcenkel, S. H., Chunwonge, J., Frary, A., Ganal, M. W., Spivey, R., Wu, T., Earle, E. D. and Tanksley, S. D. (1993): Map-based cloning of a protein kinase gene conferring sisease resistance in tomato. Science 262, 1432–1436.

    Tanksley S. D. , 'Map-based cloning of a protein kinase gene conferring sisease resistance in tomato ' (1993 ) 262 Science : 1432 -1436.

    • Search Google Scholar
  • Meyers, B. C., Kozik, A., Griego, A., Kuang, H. and Michelmore, R. W. (2003): Genome-wide analysis of NBSLRR encoding genes in Arabidopsis. Pl. Cell. 15, 809–834.

    Michelmore R. W. , 'Genome-wide analysis of NBSLRR encoding genes in Arabidopsis ' (2003 ) 15 Pl. Cell. : 809 -834.

    • Search Google Scholar
  • Michelmore, R. W. (1995): Molecular approaches to manipulation of disease resistance genes. Annu. Rev. Phytopathol. 15, 393–427.

    Michelmore R. W. , 'Molecular approaches to manipulation of disease resistance genes ' (1995 ) 15 Annu. Rev. Phytopathol. : 393 -427.

    • Search Google Scholar
  • Murray, M. and Thopson, W. F. (1980): Rapid isolation of molecular weight plant DNA. Nucl. Acids Res. 8, 4321–4325.

    Thopson W. F. , 'Rapid isolation of molecular weight plant DNA ' (1980 ) 8 Nucl. Acids Res. : 4321 -4325.

    • Search Google Scholar
  • Negi, S. S., Rajan, S. and Kumar, R. (1996): Improvement of mango by breeding. CISH Annual Report, 1995–96. Lucknow (India), pp. 9–14.

  • Noir, S., Combes, M. C., Anthony, F. and Lashermes, P. (2001): Origin, diversity and evolution of NBS-type disease-resistance gene homologues in coffee trees (Coffea L.). Mol. Genet. Genomics 265, 654–662.

    Lashermes P. , 'Origin, diversity and evolution of NBS-type disease-resistance gene homologues in coffee trees (Coffea L.) ' (2001 ) 265 Mol. Genet. Genomics : 654 -662.

    • Search Google Scholar
  • Noriega, C. D. H., Teliz, D., Mora, A. G., Rodriquez, A. J., Zavaleta, M. E., Otero C. G. and Lee, C. C. (1999): Epidemiology of mango malformation in Guerrero, Mexico with traditional and integrated management. Plant Dis. 83, 223–228.

    Lee C. C. , 'Epidemiology of mango malformation in Guerrero, Mexico with traditional and integrated management ' (1999 ) 83 Plant Dis : 223 -228.

    • Search Google Scholar
  • Peraza-Echeverria, S., Dale, J. L., Harding, R. M. and Collet, C. (2009): Molecular cloning and in silico analysis of potential Fusarium resistance genes in banana. Mol. Breed. 23, 431–443.

    Collet C. , 'Molecular cloning and in silico analysis of potential Fusarium resistance genes in banana ' (2009 ) 23 Mol. Breed. : 431 -443.

    • Search Google Scholar
  • Ploetz, R. C. and Gregory, N. F. (1993): Mango malformation in Florida: Distribution of Fusarium subglutinans in affected trees and relationships among strains within and among different orchards. Acta Hort. 341, 388–394.

    Gregory N. F. , 'Mango malformation in Florida: Distribution of Fusarium subglutinans in affected trees and relationships among strains within and among different orchards ' (1993 ) 341 Acta Hort. : 388 -394.

    • Search Google Scholar
  • Ploetz, R., Zheng, Q. I., Vazquez, A. and Abdel, S. M. A. (2002): Current status and impact of mango malformation in Egypt. Inter. J. Pest Manag. 48, 279–285.

    Abdel S. M. A. , 'Current status and impact of mango malformation in Egypt ' (2002 ) 48 Inter. J. Pest Manag. : 279 -285.

    • Search Google Scholar
  • Ram Nath, K. R. S and Sachan, I. P. (1987): Studies on mango malformation. Indian J. Mycol. Pl. Pathol. 17, 29–33.

    Sachan I. P. , 'Studies on mango malformation ' (1987 ) 17 Indian J. Mycol. Pl. Pathol. : 29 -33.

    • Search Google Scholar
  • Richter, T. E. and Ronald, P. C. (2000): The evolution of disease resistance genes. Pl. Mol. Biol. 42, 195–204.

    Ronald P. C. , 'The evolution of disease resistance genes ' (2000 ) 42 Pl. Mol. Biol. : 195 -204.

    • Search Google Scholar
  • Samuelian, S. K., Angela, M. B., Jeremy, A. P. and Courtney, A. W. (2008): Isolation and linkage mapping of NBS-LRR resistance gene analogs in red raspberry (Rubus idaeus L.) and classification among 270 Rosaceae NBS-LRR genes. Tree Genet. Genomes 4, 881–896.

    Courtney A. W. , 'Isolation and linkage mapping of NBS-LRR resistance gene analogs in red raspberry (Rubus idaeus L.) and classification among 270 Rosaceae NBS-LRR genes ' (2008 ) 4 Tree Genet. Genomes : 881 -896.

    • Search Google Scholar
  • Sharma, T. R., Kumar, Alok, Das, S. P. and Lodha, M. L. (2009): Resistance gene analogues as a tool for rapid identification and cloning of disease resistance genes in plants — a review. J. Plant Bioc. Biotech. 18, 1–11.

    Lodha M. L. , 'Resistance gene analogues as a tool for rapid identification and cloning of disease resistance genes in plants — a review ' (2009 ) 18 J. Plant Bioc. Biotech. : 1 -11.

    • Search Google Scholar
  • Song, W. Y., Wang, G. L., Chen, L. L., Kim, H. S., Pi, L. Y., Holsten, T., Gardner, J., Wang, B., Zhai, W. X., Zhu, L. H., Fauquet, C. and Ronald, P. (1995): A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science 270, 1804–1806.

    Ronald P. , 'A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21 ' (1995 ) 270 Science : 1804 -1806.

    • Search Google Scholar
  • Tan, H., Callahan, F. E., Zhang, X. D., Karaca, M., Saha, S., Jenkins, J. N., Creech, R. G. and Ma, D. P. (2003): Identification of resistance gene analogs in cotton (Gossypium hirsutum L.). Euphytica. 134, 1–7.

    Ma D. P. , 'Identification of resistance gene analogs in cotton (Gossypium hirsutum L.) ' (2003 ) 134 Euphytica. : 1 -7.

    • Search Google Scholar
  • Timmerman-Vaughan, G. M., Frew, T. J. and Weeden, N. F. (2000): Characterization and linkage mapping of R-gene analogous DNA sequences in pea (Pisum sativum L.). Theor. Appl. Genet. 101, 241–247.

    Weeden N. F. , 'Characterization and linkage mapping of R-gene analogous DNA sequences in pea (Pisum sativum L.) ' (2000 ) 101 Theor. Appl. Genet. : 241 -247.

    • Search Google Scholar
  • Xu, Q., Wen, X. and Deng, X. (2005): Isolation of TIR and non TIR NBS-LRR resistance gene analogues and identification of molecular markers linked to a powdery mildew resistance locus in chestnut rose (Rosa roxburghii Tratt). Theor. Appl. Genet. 111, 819–830.

    Deng X. , 'Isolation of TIR and non TIR NBS-LRR resistance gene analogues and identification of molecular markers linked to a powdery mildew resistance locus in chestnut rose (Rosa roxburghii Tratt) ' (2005 ) 111 Theor. Appl. Genet. : 819 -830.

    • Search Google Scholar
  • Zhang, L. P., Lin, G. Y., Nino-Liu, D. and Foolad, M. R. (2003): Mapping QTLs conferring early blight (Alternaria solani) resistance in a Lycopersicon esculentum × L. hirsutum cross by selective genotyping. Mol. Breed. 12, 3–19.

    Foolad M. R. , 'Mapping QTLs conferring early blight (Alternaria solani) resistance in a Lycopersicon esculentum × L. hirsutum cross by selective genotyping ' (2003 ) 12 Mol. Breed. : 3 -19.

    • Search Google Scholar
  • Zhou, T., Wang, T., Chen, J. Q., Araki, H., Jing, Z., Jiang, K., Shen, J. and Tian, D. (2004): Genome-wide identification of NB S genes in japonica rice reveals significant expansion of divergent non-TIR NB S-LRR genes. Mol. Genet. Genomics 271, 402–415.

    Tian D. , 'Genome-wide identification of NB S genes in japonica rice reveals significant expansion of divergent non-TIR NB S-LRR genes ' (2004 ) 271 Mol. Genet. Genomics : 402 -415.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 0 0
Jul 2020 0 1 0
Aug 2020 1 0 0
Sep 2020 2 0 0
Oct 2020 1 0 0
Nov 2020 4 14 6
Dec 2020 0 0 0