The seed-borne (Pyrenophora graminea; Pg) and foliar
(Blumeria graminis; Bg) are two economically important
fungal pathogens of barley worldwide. Barley plant resistance genes, as the
pathogenesis related proteins play an important role in defense mechanisms. This
study aimed to monitor the expression of PR2 and
PAL pathogenesis related genes during
compatible/incompatible barley interaction with Pg and
Bg at different time points of disease development using
the Quantitative Real-time PCR technique (qRT-PCR).
Comparison of data showed that PR2 and PAL were
significantly over expressed in infected resistant and susceptible plants as
against their lower expression in controls,. Upregulation of these
defense-related genes during Pg and Bg
infections was companied with a slow development of disease symptoms at the time
course in the resistant genotype. qRT-PCR analysis revealed higher gene
expression in resistant barley plants inoculated with Pg as
compared with Bg, with a maximum expression for
PR2 (13.8 and 5.06-fold) and PAL (14.8 and
4.51-fold) respectively, at the latest stage of each disease development. It was
also noteworthy that PR2 and PAL genes, had
higher constitutive expression and faster induction for the both pathogens in
the resistant genotype as compared with the susceptible one.
Obtained results suggest that both genes, PR2 and
PAL, positively regulate Pg- and
Bg-resistance in barley plants during disease progress.
These expression patterns can provide useful insights to better understanding of
the barley–fungus interactions with different fungal lifestyles.
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