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  • 1 Department of Molecular Biology and Biotechnology, AECS, P. O. Box 6091 Damascus, Syria
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Powdery mildew (Blumeria graminis) is a major fungal disease of barley causing economical yield losses worldwide. Breeding for resistance to this disease is crucial due to the rapid change in pathotype patterns of B. graminis in fields. In the present work, powdery mildew-resistant barley germplasm was developed by crossing four cultivars currently used in Europe and West Asia. Out of 265 doubled haploid lines derived from these crosses, 40 lines were evaluated at seedling and adult stages. Data showed significant differences among barley lines with a continuum of resistance levels ranging from highly susceptible to tolerant which were consistent during the two growth stages. Two promising lines were more tolerant to powdery disease than the others. Across lines, there was a high correlation between field and greenhouse reaction (r=0.80, P<0.01), indicating the utility of greenhouse evaluations for screening barley for powdery mildew. This study suggests that, the newly identified resistance lines can serve as potential donors for ongoing powdery mildew resistance breeding program, and both types of seedling and adult plant resistance identified here can offer promising genetic stocks for accumulating both resistances to acquire durable resistance and long lasting control against B. graminis in Mediterranean and similar environments.

  • Anonymous, (1988): STAT-ITCF, Programme, MICROSTA, realized by ECOSOFT, 2nd Version. Institut Technique des cereals et des Fourrages, Paris, 55 p.

    • Search Google Scholar
    • Export Citation
  • Arabi, M. I. E. and Jawhar, M. (2012): Expression of resistance to Blumeria graminis in barley genotypes (Hordeum vulgare L.) under field and controlled conditions. J. Plant Biol. Res. 2, 107112.

    • Search Google Scholar
    • Export Citation
  • Arabi, M. I. E., Al-Safadi, B., Jawhar, M. and Mir-Ali, N. (2005): Enhancement of embryogenesis and plant regeneration from barley anther culture by low doses of gamma irradiation. In Vitro Cell. Dev. Biol. 41, 762764.

    • Search Google Scholar
    • Export Citation
  • Bargougul, M. A. (2016): Diallel analysis of the resistance to powdery mildew (Erysiphe graminis f.sp. hordei ) in barley (Hordeum vulgare L.). J. New Sci. 27, 14661473.

    • Search Google Scholar
    • Export Citation
  • Boyd, A., Smith, P. H., Foster , E. M. and Brown, J. K. M. (1995): The effect of allelic variation at the Mla resistance locus in barley on the early development of Erysiphe graminis f.sp. hordei and host responses. The Plant J. 7, 959968.

    • Search Google Scholar
    • Export Citation
  • Ceccarelli, S., Grando, S., and Van Leur, J. A. G. (1995): Barley landraces of the Fertile Crescent offer new breeding options for stress environments. Diversity 11, 112113.

    • Search Google Scholar
    • Export Citation
  • Das, M. K. and Griffy, C. A. (1994): Diallel analysis of adult-plant resistance to powdery mildew in wheat. Crop Sci. 34, 948952.

  • Dreiseitl, A. (2016): Emerging Blumeria graminis f. sp. hordei pathotypes reveal ‘Psaknon’ resistance in European barley varieties. The J. Agri. Sci. 154, 10821089.

    • Search Google Scholar
    • Export Citation
  • Dreiseitl, A. and Zavřelová, M. (2018): Identification of barley powdery mildew resistances in gene bank accessions and the use of gene diversity for verifying seed purity and authenticity. PLoS ONE 13(12).

    • Search Google Scholar
    • Export Citation
  • Foroughi-Wehr, B., Mix, C., Gaul, H. and Wilson, H. M. (1976): Plant production from cultured anthers of Hordeum vulgare L . Z. Pflanzenzuchtg. 77, 198204.

    • Search Google Scholar
    • Export Citation
  • Halward, T. M. and Wynne, J. C. (1991): Generation means analysis for productivity in two diverse peanut crosses. Theor. Appl. Genet. 82, 784792.

    • Search Google Scholar
    • Export Citation
  • Hysing, S. C., Rosenqvist, H. and Wiik, L. (2012): Agronomic and economic effects of host resistance vs. fungicide control of barley powdery mildew in southern Sweden. Crop Prot. 41, 122127.

    • Search Google Scholar
    • Export Citation
  • ICARDA (1998): ICARDA Annual Report (1997): International Center for Agricultural Research in the Dry Areas, Aleppo, Syria.

    • Search Google Scholar
  • Jørgensen, J. H. (1992): Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley. Euphytica 63, 141152.

    • Search Google Scholar
    • Export Citation
  • Jørgensen, J. H. (1994): Genetics of powdery mildew resistance in barley. Crit. Rev. Plant Sci. 13, 97119.

  • Kasha, K. J. and Kao, K. N. (1970): High frequency haploid production in barley (Hordeum vulgare L.). Nature 225, 874876.

  • Koga, H, Bushnell, W. R. and Zeyen, R. J. (1990): Specificity of cell type and timing of events associated with papilla formation and the hypersensitive reaction in leaves of Hordeum vulgare attacked by Erysiphe graminis f.sp. hordei. Can. J. Bot. 68, 23442352.

    • Search Google Scholar
    • Export Citation
  • Kundsen, J. C. N., Dalsgaard, H. H. and Jørgensen, J. H. (1986): Field assessment of partial resistance to powdery mildew in spring barley . Euphytica, 35, 233243.

    • Search Google Scholar
    • Export Citation
  • Leur van, J. A. G., Ceccarrelli, S. and Grando, S. (1989): Diversity for disease resistance in barley landraces from Syria and Jordan. Plant Breed. 103, 324335.

    • Search Google Scholar
    • Export Citation
  • Moseman, J. G. and Baenziger, P. S. (1981): Genes conditioning resistance of Hordeum spontaneum to Erysiphe graminins f. sp. Hordi. Crop Sci. 21, 229232.

    • Search Google Scholar
    • Export Citation
  • Rsaliyev, A., Pahratdinova, Z. and Rsaliyev, S. (2017): Characterizing the pathotype structure of barley powdery mildew and effectiveness of resistance genes to this pathogen in Kazakhstan. BMC Plant Biol. 17, 178.

    • Search Google Scholar
    • Export Citation
  • Subrahmanyam, N. C. and Kasha, K. J. (1975): Chomosome doubling of barley haploids by nitrous oxide and colchicines treatments. Can. J. Genet. Cytol. 17, 573583.

    • Search Google Scholar
    • Export Citation
  • Wolter, M., Hollricher, K., Salamini F. and Schulze-Lefert, P. (1993): The mlo resistance alleles to powdery mildew infection in barley trigger a developmentally controlled defence mimic phenotype. Mol. Gen. Genet. 239, 122128.

    • Search Google Scholar
    • Export Citation
  • Zadoks, J. C., Chang, T. T. and Konzak, C. F. (1974): A decimal code for the growth stages of cereals. Weed Res . 14, 415421.