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  • 1 Laboratoire de Biotechnologie des Productions Végétales, Département de Biotechnologies, Faculté des Sciences de la Nature et de la Vie, Université Saad Dahleb-Blida 1, Blida, Algeria
  • 2 Laboratoire de culture in vitro, Département Central, Institut Technique de I’Arboriculture Fruitière et de la Vigne, ITAFV, Algiers, Algeria
  • 3 Laboratoire de Phytopathologie et Biologie Moléculaire, Ecole Nationale Supérieure d’Agronomie (ENSA, Rue Hassan Badi, Belfort, El Harrach, 16000, Algeria
  • 4 Laboratoire Amélioration Intégrative Des Productions Végétales (AIPV, C2711100), Département de Productions Végétales, Ecole Nationale Supérieure Agronomique (ENSA), Hassan Badi, El Harrach, Algeria
  • 5 Laboratoire Central, Service des Contrôles phytosanitaires du Centre National de Contôle et de Certification des semences et plants (CNCC), Hassan Badi, El Harrach, 16200, Algeria
  • 6 Laboratoire Ressources Génétiques et Biotechnologiques, Ecole Nationale Supérieure d’Agronomie, Rue Hassan Badi, Belfort, El Harrach, 16000, Algeria
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Several grapevine viruses were reported in Algeria and especially in grapevine germplasm collection, therefore it is a great challenge to free these varieties from virus infection before any breeding programs. Our study focused on the development of chemotherapy on autochthonous varieties collected in the grapevine germplasm collection of ITAFV. All these varieties were tested by DAS-ELISA and the presence of GLRaV-3 and GFLV was confirmed in all used samples for the sanitation. After 8 weeks of shoot tips in vitro culture in a modified M S medium containing ribavirin, DAS-ELISA test revealed that GLRaV-3 was completely eliminated and GFLV to a significant rate.

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