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  • 1 Department of Agriculture, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria
  • | 2 Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria
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Abstract

Fusarium head blight (FHB) is consistently one of the most important barley diseases worldwide. This study aimed to evaluate the pathogenicity of 16 isolates of four Fusarium species under controlled conditions and their genetic variability using 22 random amplified polymorphic DNA (RAPD) markers. Pathogenic variation was characterized based on disease development rates and disease index on two Syrian barley landraces with varying resistance to FHB, Arabi Aswad (AS) and Arabi Abiad (AB). Significant differences in intra- and inter-Fusarium species pathogenicity and in susceptibility between the above-mentioned cultivars were highlighted. Overall, the two barley landraces showed moderately susceptible to moderately resistance levels to fungal infection and FHB spread within the head. Quantitative traits showed significant correlation with previous data generated in vitro and under field conditions, suggesting that growth chamber indices can predict fungal pathogenicity and quantitative disease resistance generated under various experimental conditions. Based on PCR amplification with seven different primers, the isolates showed genetic variation. Dendrogram generated by cluster analysis based on RAPD markers data showed two main groups, suggesting that a possible clonal origin could exist in the four Fusarium species. RAPD fingerprints are not useful to distinguish the 16 Fusarium isolates with different levels of pathogenicity.

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Jenő KONTSCHÁN 
(Centre for Agricultural Research, Plant Protection Institute)

Technical editor: Ágnes TURÓCI (Centre for Agricultural Research, Plant Protection Institute)

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  • Pál BENEDEK (Hungarian University of Agriculture and Life Sciences)
  • José Antonio Hernández CORTÉS (CEBAS – Spanish National Research Council)
  • Tibor ÉRSEK (Hungarian University of Agriculture and Life Sciences)
  • Wittko FRANCKE (University of Hamburg)
  • László HORNOK (Hungarian University of Agriculture and Life Sciences)
  • József HORVÁTH (University of Pannonia, Faculty of Georgikon)
  • Mehmet Bora KAYDAN (Cukurova University)
  • Zoltán KIRÁLY (Centre for Agricultural Research, Plant Protection Institute)
  • Levente KISS (University of Southern Queensland)
  • Karl-Heinz KOGEL (University of Giessen)
  • Jenő KONTSCHÁN (Centre for Agricultural Research, Plant Protection Institute)
  • Tamás KŐMÍVES (Centre for Agricultural Research, Plant Protection Institute)
  • László PALKOVICS (Hungarian University of Agriculture and Life Sciences)
  • Miklós POGÁNY (Centre for Agricultural Research, Plant Protection Institute)
  • James E. SCHOELZ (University of Missouri)
  • Stefan SCHULZ (Technical University of Braunschweig)
  • Andrzej SKOCZOWSKI (Pedagogical University of Kraków)
  • Gábor SZŐCS (Centre for Agricultural Research, Plant Protection Institute)
  • Miklós TÓTH (Centre for Agricultural Research, Plant Protection Institute)
  • Ferenc VIRÁNYI (Hungarian University of Agriculture and Life Sciences)
  • Pedro Díaz VIVANCOS (CEBAS – Spanish National Research Council)

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2020  
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2019  
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212
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Acta Phytopathologica et Entomologica Hungarica
Language English
Size B5
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1966
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2020 Volume 55
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per Year
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2
Founder Magyar Tudományos Akadémia
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ISSN 0238-1249 (Print)
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