Effects of Meloidogyne incognita, Pseudomonas syringae pv. pisi and Rhizobium leguminosarum were studied on growth and biochemical parameters of pea (Pisum sativum L.) in three soil types. Plants grown in 20% fly ash attained higher plant growth, chlorophyll and carotenoid followed by loam soil and 20% sand. Inoculation of R. leguminosarum resulted in increased plant growth, nodulation, chlorophyll and carotenoid over control. Root nodulation and proline contents were high in plants grown in 20% sand and least in 20% fly ash. Inoculation of M. incognita prior to P. syringae pv. pisi resulted in a greater reduction in plant growth, nodulation, chlorophyll and carotenoid content and least where P. syringae pv. pisi was inoculated prior to M. incognita. Inoculation of pathogens increased proline contents. Galling and population of M. incognita was high in 20% sand followed by loam soil and fly ash amended soil. P. syringae pv. pisi and R. leguminosarum had adverse effect on galling and nematode population. The principal component analysis identifies interaction of pathogens and showed segregation of various treatments in the plots.
Ahmad, L. and Siddiqui, Z.A. (2017). Effects of Meloidogyne incognita, Alternaria dauci and Fusarium solani on carrot in different types of soil. Acta Phytopathologica. et Entomologica Hungarica, 52: 39–47.
Ali, M.A., Trabulsi, 1.Y., and Abd-Elsamea, M.E. (1981). Antagonistic interaction between Meloidogyne incognita and Rhizobium leguminosarum on cowpea. Plant Disease, 65: 432–435.
Arora, N.K., Kang, S.C., and Maheshwari, D.K. (2001). Isolation of siderophore producing strains of Rhizobium meliloti and their biocontrol potential against Macrophomina phaseolina that causes charcoal rot of groundnut. Current Science, 81: 673–677.
Back, M.A., Haydock, P.P.J., and Jenkinson, P. (2002). Disease complexes involving plant parasitic nematodes and soil-borne pathogens. Plant Pathology, 51: 683–697.
Bardin, S.D., Huang, H.C., Pinto, J., Amundsen, E.J., and Erickson, R.S. (2004). Biological control of Pythium damping-off of pea and sugar beet by Rhizobium leguminosarum bv. viceae. Canadian Journal of Botany, 82: 291–296.
Barker, K.R., Huising, D., and Johnston, S.A. (1972). Antagonistic interaction between Heterodera glycines and Rhizobium japonicum on soybean. Phytopathology, 62: 1201–1205.
Basu, M., Pande, M., Bhadoria, P.B.S., and Mahapatra, S.C. (2009). Potential fly-ash utilization in agriculture: A global review. Progress in Natural Science, 19: 1173–1186.
Bates, L.S., Waldren, R.T., and Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39: 205–207.
Begon, M., Townsend, C.R., and Harper, J.L. (2006). Ecology, individuals, populations and communities, 4th ed. Blackwell Science, London, p. 759.
Carson, K.C., Meyer, J.M., and Dilworth, M.J. (2000). Hydroxamate siderophore of root nodule bacteria. Soil Biology and Biochemistry, 32: 11–21.
Cecchini, N.M., Monteoliva, M.I., and Alvarez, M.E. (2011). Proline dehydrogenase contributes to pathogen defense in Arabidopsis. Plant Physiology, 155: 1947–1959.
Chahal, P.P.K., Singh, I., and Chahal, V.P.S. (1983). Interaction between different population levels of Meloidogyne incognita and Rhizobium on green gram. Journal of Research Punjab Agricultural University, 20: 399–402.
Chahal, P.P.K., Singh, I., and Chhabra, J.K. (1985). Effect of Meloidogyne incognita and Rhizobium on growth of mungbean. Journal of Research Punjab Agricultural University, 22: 181–183.
Chandra, S., Choure, K., Dubey, R.C., and Maheshwari, D.K. (2007). Rhizosphere competent Mesorhizobium loti MP6 induces root hair curling, inhibits Sclerotinia sclerotiorum and enhances growth of Indian mustard (Brassica campestris). Brazilian Journal of Microbiology, 38: 128–130.
Chopra, S.L. and Kanwar, J.S. (1982). Analytical agricultural chemistry .Kalyani, New Delhi, p. 162.
Deshwal, V.K., Dubey R.C., and Maheshwari, D.K. (2003a). Isolation of plant growth promoting strains of Bradyrhizobium (Arachis) sp. with biocontrol potential against Macrophomina phaseolina causing charcoal rot of peanut. Current Science, 84: 443–444.
Deshwal, V.K., Pandey, P., Kang, S.C., and Maheshwari, D.K. (2003b). Rhizobia as a biological control agent against soil borne plant pathogenic fungi. Indian Journal of Experimental Biology, 41: 1160–1164.
Dhall, R.K. (2017). Pea cultivation book. Printed at PAU Printing Press, Ludhiana. Punjab, India. Bulletin No. PAU/2017/Elec/FB/. ISBN: 978-93-86267-37-5.
Doney, D.L., Fife, J.M., and Whitney, E.D. (1970). The effects of sugarbeet nematode Heterodera schachtii on the free amino acids in resistant and susceptible Beta species. Phytopathology, 60: 1727–1729.
Dubey, P. S., Pawar, K., and Trivedi, L. (1982). Effect of fly ash deposition on photosynthetic pigment and dry matter production of wheat and gram. Agro-Ecosystem, 8: 137–140.
Fabro, G., Kovacs, I., Pavet, V., Szabados, L., and Alvarez, M.E. (2004). Proline accumulation and AtP5CS2 gene activation are induced by plant-pathogen incompatible interactions in Arabidopsis. Molecular Plant Microbe Interactions, 17: 343–350.
Francois, L.E. (1984). Effect of excess boron on tomato yield, fruit size and vegetable growth. Journal of American Society of Horticulture Science, 109: 322–324.
Garbeva, P., van Veen, J.A., and van Elsas, J.D. (2004). Microbial diversity in soil: selection of microbial populations by plant and soil type and implications for disease suppressiveness. Annual Review of Phytopathology, 42: 243–270.
Gill, J.S. (1989). Nematodes associated with pulse crops. In: Proceeding of All India Nematology Workshop on Pulses and oil seed crops. Udaipur, Rajasthan, pp. 1–8.
Gopalakrishnan, S., Sathya, A., Vijayabharathi, R., Varshney, R.K., Gowda, C.L.L., and Krishnamurthy, L. (2015). Plant growth promoting rhizobia: challenges and opportunities. 3 Biotech, 5(4): 355–377.
Hagedorn, D.J. (1991). Hand book of pea diseases .University of Wisconsin, Madison Extension, Bulletin A1167, p. 35.
Harmankaya, M., Özcan, M.M., Kardas, S., and Ceyhan, E. (2010). Protein and mineral contents of pea (Pisum sativum L.) genotypes grown in central Anatolian region of Turkey. South – Western Journal of Horticulture Biology and Environment, 1(2): 159–165.
Hazarika, K. (2003). Interrelationship of Meloidogyne incognita and Pseudomonas solanacearum on jute and management of the disease complex caused by them. Ph.D. (Nematology) Thesis, submitted to Assam Agricultural University Johrat-13, India.
Hussain, Z. and Bora, B.C. (2009): Interrelationship of Meloidogyne incognita and Ralstonia solanacearum complex in brinjal. Indian Journal of Nematology, 39: 41–45.
Jackson, M.L. (1958). Soil chemical analysis .Prentice hall, Englewood Cliffs, NJ, p. 498.
Khan, M.R. and Khan, M.W. (1996). Effect of fly ash on plant growth and yield of tomato. Environmental Pollution, 92: 105–112.
Khan, M.R., Khan, M.W., and Singh, K. (1997). Management of root-knot disease of tomato by the application of fly ash in soil. Plant Pathology, 46: 33–43.
Khan, M. and Siddiqui, Z.A. (2017). Interactions of Meloidogyne incognita, Ralstonia solanacearum and Phomopsis vexans on eggplant in sand mix and fly ash mix soils. Scientia Horticulturae, 225: 177–184.
Mackinney, G. (1941). Absorption of light by chlorophyll solutions. Journal of Biological Chemistry, 140: 315–322.
Malek, R.B. and Jenkins, W.R. (1964). Aspects of host-parasite relationship of nematodes and Hairy vetch. New Jersey Agricultural Experiment Station Bulletin, 813: 31.
Mallesh, S. B., Lingraju, S., Byadgi, A. S., Hegde, Y. R., Mokashi, A. N., and Krishnaraj, P. U. (2009). Bioefficacy of rhizobacteria on root-knot/wilt disease complex in coleus and ashwganda. Karnataka Journal of Agricultural Science, 22: 1116–1120.
Martín-Sanz, A., De LaVega, M.P., Murillo, J., and Caminero, C. (2013). Strains of Pseudomonas syringae pv. syringae from pea are phylogenetically and pathogenically diverse. Phytopathology, 103(7): 673–681.
Minchin, R.L. and Pate, J.S. (1973). The carbon balance of a legume and the functional economy of its root nodules. Journal of Experimental Botany, 24: 259–271.
Nelson, D.W. and Sommers, L.F. (1972). A simple digestion procedure for estimation of total nitrogen in soil and sediments. Journal of Environmental Quality, 1: 423–425.
Nesha, R. and Siddiqui, Z.A. (2013). Interactions of Pectobacterium carotovorum pv. carotovorum, Xanthomonas campestris pv. carotae, and Meloidogyne javanica on the disease complex of carrot. International Journal of Vegetable Science, 19(4): 403–411.
Ozkoc, I. and Deliveli, M.H. (2001). In vitro inhibition of the mycelial growth of some root rot fungi by Rhizobium leguminosarum biovar phaseoli isolates. Turkish Journal of Biology, 25: 435–445.
Panda, R.B. and Biswal, T. (2018). Impact of fly ash on soil properties and productivity. International Journal of Agriculture Environment and Biotechnology, 11(2): 275–283.
Parveen, G., Noreen, R., Shafique, H.A., Sultana, V., Haque, S.E., and Athar, M. (2019). Role of rhizobia in suppressing the root diseases of soybean under soil amendment. Planta daninha, 37: https://doi.org/10.1590/s0100-83582019370100038.
Ravichandra, N.G. (2014). Horticultural Nematology. Springer, New Delhi, India, pp. 1–412.
Reddy, P.P. (1985). Estimation of crop losses in pea due to Meloidogyne incognita. Indian Journal of Nematology, 15: 226.
Rubio-Cabetas, M., Minot, J., Voisin, R., and Esmenjaud, D. (2001). Interaction of root-knot nematodes (RKN) and the bacterium Agrobacterium tumefaciens in roots of Prunus cerasifera: evidence of the protective effect of the Ma RKN resistance genes against expression of crown gall symptoms. European Journal of Plant Pathology, 107: 433–441.
Rungruangmaitree, R. and Jiraungkoorskul, W. (2017). Pea, Pisum sativum, and its anticancer activity. Pharmacognosy Reviews, 11(21): 39–42.
Sarangi, P. K., Mishra, T. K., and Mishra, P. C. (1997). Soil metabolism, growth and yield of Oryza sativa L. in fly ash amended soil. Indian Journal of Environmental Science, 1: 17–24.
Schmit, J, Cousin, R., and Rousseau, M.T. (1992). Race distribution of Pseudomonas syringae pv. pisi in relation to susceptibility of cultivars of protein peas in France. In: Plancquaert, P. (Ed.), Proceeding of 1st European Conference on Grain Legumes, Angers, France, pp. 335–336.
Senthil-Kumar, M. and Mysore, K.S. (2012). Ornithine-delta-aminotransferase and proline dehydrogenase genes play a role in non-host disease resistance by regulating pyrroline-5-carboxylate metabolism-induced hypersensitive response. Plant Cell and Environment, 35: 1329–1343.
Sharma, G.L. (1989). Estimated losses due to root-knot nematode Meloidogyne incognita and M. javanica in pea crop. International Nematology Network Newsletter, 6: 28–29.
Sharma, P.D. (2005). Microbiology. Rastogi and Company, Meerut, India, pp. 1–539.
Siddiqui, Z.A. and Mahmood, I. (2001). Effects of rhizobacteria and root symbionts on the reproduction of Meloidogyne javanica and growth of chickpea. Bioresource Technology, 79: 41–45.
Siddiqui, Z.A., Nesha, R., Singh N., and Alam S. (2012). Interactions of plant parasitic nematodes and plant pathogenic bacteria. In: Maheshwari, D.K. (Ed.), Bacteria in Agrobiology. Plant Probiotics, Springer-Verlag Berlin Heidelberg, pp. 251–267. ISBN .
Sistani, N.R., Kaul, H.P., Desalegn, G., and Wienkoop, S. (2017). Rhizobium impacts on seed productivity, quality, and protection of Pisum sativum upon disease stress caused by Didymella pinodes: Phenotypic, Proteomic, and Metabolomic Traits. Frontiers in Plant Science, 8: 1–15. https://doi.org/10.3389/fpls.2017.01961.
Sitaramaiah, K. and Pathak, K.N. (1993). Nematode bacterial disease interactions. In: Khan, M.W. (Ed.), Nematode interactions , pp. 232–250. Chapman and Hall, New York, pp. 1–377.
Slack, D.A. (1963): Introduction. Symposium of interrelationships between nematodes and other agents causing plant diseases. Phytopathology, 53: 27–47.
Sneath, P.H. and Sokal, R.R. (1973). Numerical taxonomy. The principles and practice of numerical classification, W. H. Freeman and Company, San Francisco, USA, pp. 1–573.
Southey, J.F. (1986): Laboratory methods for work with plant and soil nematodes. Ministry of Agric. Fisheries and Food, Her Majesties Stationary Office, London, pp. 1–202.
Swain, P.K., Rath, J.C., and Mishra, S.K. (1987). Interaction between Meloidogyne incognita and Pseudomonas solanacearum on brinjal. Indian Journal of Nematology, 17: 61–71.
Trudgill, D.L. and Phillips, M.S. (1997). Nematode population dynamics, threshold levels and estimation of crop losses. Plant Production and Protection Paper, Food and Agriculture Organization, 144, Rome.
Upadhyay K.D. and Dwivedi K. (1987). Analysis of crop losses in pea and gram due to Meloidogyne incognita. International Nematology Network Newsletter, 4: 6–7.
Verslues, P.E. and Sharma, S. (2010). Proline metabolism and its implications for plant-environment interaction. Arabidopsis Book, 8: e0140. Published online Nov 3. https://doi.org/10.1199/tab.0140. PMCID: PMC3244962, PMID: 22303265.
Vovlas, N., Rapoport, H.F., Jiménez Díaz, R.M., and Castillo, P. (2005). Differences in feeding sites induced by root-knot nematodes, Meloidogyne spp., in chickpea. Phytopathology, 95: 368–375.