In our study, we examined mycotoxin-producing fungi found in sorghum grains (Sorghum bicolor L. Moench). During the experiment, the internal infection of grains was examined on 3 different selective culture media, as individual literature differs as to which selective culture media are the most suitable for the isolation of Fusarium species under laboratory conditions (Leslie and Summerell, 2006). During our experiments, we also used molecular methods to identify the selected Fusarium species down to the species level. In the case of Fusarium species our experiment shows that the best media is Nash & Snyder media. During the morphological identification we worked with 18 Fusarium monosporal cultures and we detected Fusarium avenaceum.
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