Authors:
Barbara Katalin Szabó Department of Integrated Plant Protection, Plant Protection Institute, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary

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Klaudia Dóra Molnár Department of Integrated Plant Protection, Plant Protection Institute, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary

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Sára Dóra Vlaskality Department of Integrated Plant Protection, Plant Protection Institute, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary

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Katalin Körösi Department of Integrated Plant Protection, Plant Protection Institute, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary

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Abstract

In our study, we examined mycotoxin-producing fungi found in sorghum grains (Sorghum bicolor L. Moench). During the experiment, the internal infection of grains was examined on 3 different selective culture media, as individual literature differs as to which selective culture media are the most suitable for the isolation of Fusarium species under laboratory conditions (Leslie and Summerell, 2006). During our experiments, we also used molecular methods to identify the selected Fusarium species down to the species level. In the case of Fusarium species our experiment shows that the best media is Nash & Snyder media. During the morphological identification we worked with 18 Fusarium monosporal cultures and we detected Fusarium avenaceum.

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  • Astoreca, A.L., Lucia G., Emateguy, L.G., and Alconada, T.M. (2019). Fungal contamination and mycotoxins associated with sorghum crop: its relevance today. European Journal of Plant Pathology, 155(2): 381392.

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  • Booth, C. (1971). The genus Fusarium. Commonwealth Agricultural Bureaux for the Commonwealth Mycological Institute, Michigan University, USA, pp. 1237.

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  • Gautier, C., Pinson-Gadais, L., and Richard-Forget, F. (2020). Fusarium mycotoxins enniatins: an updated review of their occurrence, the producing Fusarium species, and the abiotic determinants of their accumulation in crop harvests. Journal of Agricultural and Food Chemistry, 68(17): 47884798.

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  • Geiser, D.M., del Mar Jiménez-Gasco, M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T.J., Zhang, N., Kuldau, G.A., and O’Donnell, K. (2004). FUSARIUM-ID v 1.0.: a DNA sequence database for identifying Fusarium. European Journal of Plant Pathology, 110: 473479.

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  • Hornok, L., Békési, P., Giczey, G., Jeney, A., Nicholson, P., Parry, D., Ritieni, A., and Xu, X. (2005). Kalászfuzáriózis-kórokozók előfordulása és a mikotoxin-szennyeződés mértéke magyarországi őszibúza-állományokban 2001–2004 között. Növénytermelés, 54(4): 217235. [in Hungarian].

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  • Leslie, J.F., Pearson, C.S.A., Nelson, P.E., and Toussoun, T.A. (1990). Fusarium spp. from corn, sorghum and soybean fields in the Central and Eastern United States. Phytopathology, 80: 343350.

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  • Leslie, J.F. and Summerell, B.A. (2006). The Fusarium laboratory manual. Blackwell Publishing Ltd, Oxford, UK, pp. 1416.

  • Mulè, G., Susca, A., Stea, G., and Moretti, A. (2004). A species-specific PCR assay based on the calmodulin partial gene for identification of Fusarium verticillioides, F. proliferatum and F. subglutinans. European Journal of Plant Pathology, 110(5): 495502.

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  • Nagaraja, H., Chennappa, G., Poorna Chandra Rao, K., Mahadev Prasad, G., and Sreenivasa, M.Y. (2016). Diversity of toxic and phytopathogenic Fusarium species occurring on cereals grown in Karnataka state, India. 3 Biotech, 6(1): 18.

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  • Nicholson, P., Simpson, D.R., Weston, G., Rezanoor, H.N., Lees, A.K., Parry, D.W., and Joyce D. (1998). Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology, 53(1): 1737.

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  • Parry, D.W. and Nicholson, P. (1996). Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathology, 45: 383391. https://doi.org/10.1046/j.1365-3059.1996.d01-133.x.

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  • Peerzada, A.M., Ali, H.H., and Chauhan, B.S. (2017). Weed management in sorghum [Sorghum bicolor (L.) Moench] using crop competition: a review. Crop Protection, 95(1): 7480.

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  • Pena, G.A., Cavaglieri, L.R., and Chulze, S.N. (2019). Fusarium species and moniliformin occurrence in sorghum grains used as ingredient for animal feed in Argentina. Journal of the Science of Food and Agriculture, 99(1): 4754.

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  • Ponts, N., Gautier, C., Gouzy, J., Pinson-Gadais, L., Foulongne-Oriol, M., Ducos, C., Richard-Forget, F., Savoie, J.-M., Zhao, C., and Barroso, R. (2020). Evolution of Fusarium trinticum and Fusarium avenaceum mitochondrial genomes is driven by mobility of introns and of a new type of palindromic microsatellite repeats. BMC Genomics, 21: 116.

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  • Serna-Saldivar, S.O. (2016). Cereal grains: properties, processing, and nutritional attributes. CRC Press, Taylor and Francis Group, UK, pp. 1794.

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  • Sorghum ID – európai, szakmaközi cirokszervezet: L’avenir mise sur le sorgho. https://www.sorghum-id.com/ (accessed: October 10, 2022).

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  • Turner, A.S., Lees, A.K., Rezanoor, H.N., and Nicholson, P. (1998). Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathology, 47(3): 278288.

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  • Varga, J., Téren, J., Tóth, B., Kocsubé, S., és Rigó, K. (2009). Gombák másodlagos anyagcseretermékei: Mikotoxinok, gombamérgek. JATEPress, Szeged, Hungary, pp. 1114.

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Editor-in-Chief

Jenő KONTSCHÁN Centre for Agricultural Research, Hungary

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Ágnes TURÓCI Centre for Agricultural Research, Hungary

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Z BOZSÓ Centre for Agricultural Research, Hungary
PE CHETVERIKOV Saint-Petersburg State University, Russia
JX CUI Henan Institute of Science and Technology, China
J FODOR Centre for Agricultural Research, Hungary
Z IMREI Centre for Agricultural Research, Hungary
BM KAYDAN Çukurova University, Turkey
L KISS University of Southern Queensland, Australia
V MARKÓ Hungarian University of Agriculture and Life Sciences, Hungary
MW NEGM Ibaraki University, Japan
L PALKOVICS Széchenyi István University, Hungary
M POGÁNY Centre for Agricultural Research, Hungary
D RÉDEI National Chung Hsing University, Taiwan
A TOLSTIKOV University of Tyumen, Russia
J VUTS Rothamsted Research, UK
GQ WANG Guangxi University, China

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Acta Phytopathologica et Entomologica Hungarica
Language English
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1966
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Founder Magyar Tudományos Akadémia  
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