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  • 1 Department of Brewing and Distilling, Faculty of Food Science, Szent István University H-1118 Budapest, Ménesi út 45. Hungary
  • 2 Department of Brewing and Distilling, Faculty of Food Science, Szent István University H-1118 Budapest, Ménesi út 45. Hungary
  • 3 Department of Brewing and Distilling, Faculty of Food Science, Szent István University H-1118 Budapest, Ménesi út 45. Hungary
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a-Galactosidase activity from Thermomyces lanuginosus strain of CBS 395.62/b was investigated in cultivation media with various compositions. Among the seven nitrogen sources only three L-asparagine, yeast extract and ammonium acetate supported the a-galactosidase synthesis. Ammonium acetate proved to be the best candidate as nitrogen source. When glucose or galactose was used as main carbon source, very low level, constitutive a-galactosidase activity was observed. In presence of raffinose, considerable a-galactosidase activity was detected. Raffinose can be replaced by sucrose in the cultivation medium, because the productivity reached by it was superior to that of raffinose. a-Galactosidase activity was improved by the optimisation of the concentrations of sucrose and ammonium acetate in the medium. Applying medium composition with 3% (w/v) sucrose and 0.6% (w/v) ammonium acetate led to at least 5 times higher activity which was observed in the reference medium containing 1.5% (w/v) raffinose and 0.45% (w/v) L-asparagine.

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