Mineral composition of grain of some new winter wheat genotypes in Croatia was studied. The following genotypes were selected: Lara, Lenta Kruna Fiesta, AG-45 and Perla which were taken from macrotrials during two vegetation periods of 1997/98 and 1998/99 at two locations in Donji Miholjac and Kutjevo. N-concentration was determined by the micro-Kjeldahl method, P spectrophotometrically and concentration of K, Ca, Mg, Zn, Cu, Fe and Mn by the AAS method (atomic absorption spectrophotometry). Interaction between the examined parameters was defined by multiple regression and correlation analysis. The results showed higher concentrations of N, P, K, Mg, Zn, Cu, and Mn, except for Fe, in the grain from Donji Miholjac than from Kutjevo. High concentration variability of macro and microelements in the grain of the examined genotypes depended on location and growing year. Concentration of macro and microelements in the grain did not have significant correlation with the grain yield for the examined genotypes, apart from N and Mg concentration.