View More View Less
  • 1 Department of Food Microbiology and Food Hygiene, National Institute of Food Hygiene and Nutrition H-1476 Budapest, P.O. Box 52. Hungary
  • 2 Center for Food Safety and Applied Nutrition, US. Food and Drug Administration 5100 Paint Branch Parkway, College Park, MD 20740-3835. USA
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

Lactic acid bacteria isolated from commercially produced alfalfa sprouts were screened for activity against Listeria monocytogenes F4258. Most active isolates were identified as Lactococcus lactis subsp. lactis. The isolates fell into two categories, strains that inhibited by acid production only, and strains that appeared to have additional inhibitory activity. An acid-only isolate, SP26, was used to evaluate the effect of initial pH (5, 6, 7, 8) and temperature (10, 20, and 30 °C) on the interaction between the lactic acid bacterium and L. monocytogenes using “sprout juice” as a model system. The model system was inoculated with an initial level of approx. 103 CFU ml-1L. monocytogenes in both mono-culture controls and the co-cultures and the co-cultures with L. lactis (103-104 CFU ml-1). The primary inhibitory effect attributable to L. lactis was a 2 to 3 log cycle decrease in the maximum population density obtained by L. monocytogenes. The extent of the inhibition was decreased at 10 °C, but was only slightly affected by pH in the range of 6.0 to 8.0. L. monocytogenes did not grow in the sprout broth at pH 5.0 at any of the incubation temperatures.

  • Sizmur, K. & Walker, C. W. (1988): Listeria in prepacked salads. Lancet, 1, 1167.

    'Listeria in prepacked salads. ' () 1 Lancet : 1167.

  • Splittstoesser, D. F., Queale, D. T. & Andaloro, B. W. (1983): The microbiology of vegetable sprouts during commercial production. J. Fd Safety, 5, 79-86.

    'The microbiology of vegetable sprouts during commercial production. ' () 5 J. Fd Safety : 79 -86.

    • Search Google Scholar
  • Quinto, E. J., Franco, C. M., Fente, C. A., Vazquez, B. I. & Cepeda, A. (1996): Effects of Pseudomonas fluorescens on the growth of Listeria monocytogenes and Listeria innocua in skimmed milk. Arch. Lebensmittelhyg., 47, 107-110.

    'Effects of Pseudomonas fluorescens on the growth of Listeria monocytogenes and Listeria innocua in skimmed milk. ' () 47 Arch. Lebensmittelhyg. : 107 -110.

    • Search Google Scholar
  • Propokovich, D. & Blank, G. (1991): Microbiological evaluation of vegetable sprouts and seeds. J. Fd Prot., 54, 560-562.

    'Microbiological evaluation of vegetable sprouts and seeds. ' () 54 J. Fd Prot. : 560 -562.

    • Search Google Scholar
  • Shelef, L. A. (1994): Antimicrobial effects of lactates: A review. J. Fd Prot., 57, 445-450.

    'Antimicrobial effects of lactates: A review. ' () 57 J. Fd Prot. : 445 -450.

  • Patterson, J. E. & Woodburn, M. J. (1980): Klebsiella and other bacteria on alfalfa and bean sprouts at the retail level. J. Fd Sci., 45, 492-495.

    'Klebsiella and other bacteria on alfalfa and bean sprouts at the retail level. ' () 45 J. Fd Sci. : 492 -495.

    • Search Google Scholar
  • Pönka, A., Andersson, Y., Siitonen, A., De Jong, B., Jahkola, M., Haikala, O., Kuhmonen, A. & Pakkala, P. (1995) Salmonella in alfalfa sprouts. Lancet, 345, 462-463.

    'Salmonella in alfalfa sprouts. ' () 345 Lancet : 462 -463.

  • Breidt, F. & Fleming, H. P. (1998): Modeling of the competitive growth of Listeria monocytogenes and Lactococcus lactis in vegetable broth. Appl. environm. Microbiol., 64, 3159-3165.

    'Modeling of the competitive growth of Listeria monocytogenes and Lactococcus lactis in vegetable broth. ' () 64 Appl. environm. Microbiol. : 3159 -3165.

    • Search Google Scholar
  • Buchanan, R. L. & Bagi, L. K. (1997): Microbial competition: Effect of culture conditions on the suppression of Listeria monocytogenes Scott A by Carnobacterium piscicola. J. Fd Prot., 60, 254-261.

    'Microbial competition: Effect of culture conditions on the suppression of Listeria monocytogenes Scott A by ' () 60 Carnobacterium piscicola. J. Fd Prot. : 254 -261.

    • Search Google Scholar
  • Buchanan, R. L. & Bagi, L. K. (1999): Microbial competition: Effect of Pseudomonas fluorescens on the growth of Listeria monocytogenes. Fd Microbiol., 16, 523-529.

    'Microbial competition: Effect of Pseudomonas fluorescens on the growth of Listeria monocytogenes. ' () 16 Fd Microbiol. : 523 -529.

    • Search Google Scholar
  • Cai, Y., Ng, L.-K. & Farber, Y. M. (1997): Isolation and characterisation of nisin producing Lactococcus lactis subsp. lactis from bean-sprouts. J. appl. Microbiol., 83, 499-507.

    'Isolation and characterisation of nisin producing Lactococcus lactis subsp. lactis from bean-sprouts. ' () 83 J. appl. Microbiol. : 499 -507.

    • Search Google Scholar
  • Centers For Disease Control And Prevention (1997): Outbreaks of Escherichia coli O157:H7 infection associated with eating alfalfa sprouts - Michigan and Virginia, June-July 1997. Morbid. Mortal. Weekly Rep., 46, 741-744.

  • Damert, W. C. (1994): ABACUS: Interactive program for non-linear regression analysis. QCPE Bulletin, 14, 61.

    'ABACUS: Interactive program for non-linear regression analysis. ' () 14 QCPE Bulletin : 61.

    • Search Google Scholar
  • Farber, J. M., Sanders, G. W. & Johnston, M. A. (1989): A survey of various foods for the presence of Listeria species. J. Fd Prot., 52, 456-458.

    'A survey of various foods for the presence of Listeria species. ' () 52 J. Fd Prot. : 456 -458.

    • Search Google Scholar
  • Gibson, A., Bratchell, N. & Roberts, T. A. (1988): Predicting microbial growth: Growth responses of salmonellae in a laboratory medium as affected by pH, sodium chloride, and storage temperature. Int. J. Fd Microbiol., 6, 155-178.

    'Predicting microbial growth: Growth responses of salmonellae in a laboratory medium as affected by pH, sodium chloride, and storage temperature. ' () 6 Int. J. Fd Microbiol. : 155 -178.

    • Search Google Scholar
  • Hara-Kudo, Y., Konuma, H., Iwaki, M., Kasuga, F., Sugita-Konishi, Y., Ito, Y. & Kumagai, S. (1997): Potential hazard of radish sprouts as a vehicle of Escherichia coli O157:H7. J. Fd Prot., 60, 1125-1127.

    'Potential hazard of radish sprouts as a vehicle of Escherichia coli O157:H7. ' () 60 J. Fd Prot. : 1125 -1127.

    • Search Google Scholar
  • Harris, L. J., Daeschel, M. A., Stiles, M. E. & Klaenhammer, T. R. (1989): Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes. J. Fd Prot., 52, 384-387.

    'Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes. ' () 52 J. Fd Prot. : 384 -387.

    • Search Google Scholar
  • Heisick, J. E., Wagner, D. E., Nierman, M. L. & Peeler, J. T. (1989): Listeria spp. found on fresh market produce. Appl. environ. Microbiol., 55, 1925-1927.

    'Listeria spp. found on fresh market produce. ' () 55 Appl. environ. Microbiol. : 1925 -1927.

    • Search Google Scholar
  • Holzapfel, W. H., Geisen, R. & Schillinger, U. (1995): Biological preservation of foods with reference to protective cultures, bacteriocins and food grade enzymes. (Review) Int. J. Fd Microbiol., 24, 343-362.

    'Biological preservation of foods with reference to protective cultures, bacteriocins and food grade enzymes. (Review) ' () 24 Int. J. Fd Microbiol. : 343 -362.

    • Search Google Scholar
  • Itoh, Y., Sugita-Konishi, Y., Kasuga, F., Iwaki, M., Hara-Kudo, Y., Saito, N., Noguchi, Y., Konuma, H. & Kumagai, S. (1998): Enterohemorrhagic Escherichia coli O157:H7 present in radish sprouts. Appl. environ. Microbiol., 64, 1532-1535.

    'Enterohemorrhagic Escherichia coli O157:H7 present in radish sprouts. ' () 64 Appl. environ. Microbiol. : 1532 -1535.

    • Search Google Scholar
  • Mahon, B. E., Pönka, A., Hall, W. N., Komatsu, K., Dietrich, S. E., Siitonen, A., Cage, G., Hayes, P. S., Lambert-Fair, M. A., Bean, N. H., Griffin, P. M. & Slutsker, L. (1997): An international outbreak of Salmonella infections caused by alfalfa sprouts grown from contaminated seeds. J. infectious Dis., 175, 876-882.

    'An international outbreak of Salmonella infections caused by alfalfa sprouts grown from contaminated seeds. ' () 175 J. infectious Dis. : 876 -882.

    • Search Google Scholar
  • Buchanan, R. L., Stahl, H. G. & Whiting, R. C. (1989): Effects and interactions of temperature, pH, atmosphere, sodium chloride, and sodium nitrite on the growth of Listeria monocytogenes. J. Fd Prot., 52, 844-851.

    'Effects and interactions of temperature, pH, atmosphere, sodium chloride, and sodium nitrite on the growth of Listeria monocytogenes. ' () 52 J. Fd Prot. : 844 -851.

    • Search Google Scholar
  • Buchanan, R. L., Whiting, R. C. & Damert, W. C. (1997): When is simple good enough: a comparison of the Gompertz, Baranyi, and three phase linear models for fitting bacterial growth curves. Fd Microbiol., 14, 313-326.

    'When is simple good enough: a comparison of the Gompertz, Baranyi, and three phase linear models for fitting bacterial growth curves. ' () 14 Fd Microbiol. : 313 -326.

    • Search Google Scholar
  • Kelly, W. J., Davey, G. P. & Ward, L. J. H. (1998): Characterization of lactococci isolated from minimally processed fresh fruit and vegetables. Int. J. Fd Microbiol., 45, 85-92.

    'Characterization of lactococci isolated from minimally processed fresh fruit and vegetables. ' () 45 Int. J. Fd Microbiol. : 85 -92.

    • Search Google Scholar
  • Lewus, C. B., Kaiser, A. & Montville, T. J. (1991): Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Appl. environ. Microbiol., 57, 1683-1688.

    'Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. ' () 57 Appl. environ. Microbiol. : 1683 -1688.

    • Search Google Scholar
  • Pálmai, M. & Buchanan, R. L. (2002): Growth of Listeria monocytogenes during germination of alfalfa sprouts. Fd Microbiol., 19, 195-200.

    'Growth of Listeria monocytogenes during germination of alfalfa sprouts. ' () 19 Fd Microbiol. : 195 -200.

    • Search Google Scholar
  • Aabo, S. & Baggesen, D. L. (1997): Growth of Salmonella Newport in naturally contaminated alfalfa sprouts and estimation of infectious dose in a Danish Salmonella Newport outbreak due to alfalfa sprouts. Salmonella and Salmonellosis '97, 425-426.

    Salmonella and Salmonellosis '97 , () 425 -426.

  • Becker, B. & Holzapfel, W. H. (1997): Microbiological risk of prepacked sprouts and measures to reduce total counts. Arch. Lebensmittelhyg., 48, (4) 81-84.

    'Microbiological risk of prepacked sprouts and measures to reduce total counts ' () 48 Arch. Lebensmittelhyg. : 81 -84.

    • Search Google Scholar
  • Beuchat, L. R. (1995): Pathogenic microorganisms associated with fresh produce. J. Fd Prot., 59, 204-216.

    'Pathogenic microorganisms associated with fresh produce. ' () 59 J. Fd Prot. : 204 -216.

  • Beuchat, L. R. (1996): Listeria monocytogenes: incidence on vegetables. Fd Control, 7, 223-228.

    'Listeria monocytogenes: incidence on vegetables. ' () 7 Fd Control : 223 -228.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 2 0
Jul 2020 2 0 0
Aug 2020 2 0 0
Sep 2020 0 0 0
Oct 2020 1 0 0
Nov 2020 2 1 0
Dec 2020 0 0 0