Applying antagonistic yeasts is one of the recent possibilities for controlling postharvest disease caused by blue mould (Penicillium expansum). In this work, antagonistic activity of several Kl. lactisstrains was tested against two strains of P. expansum. Three strains of Kl. lactiswere compared to three biocontrol yeasts: Metschnikowia pulcherrima, Sporobolomyces roseusand Pichia anomala. The investigations were carried out at 25 °C, 15 °C and 5 °C, applying different yeast cell densities and culture media. Statistical analysis showed no significant differences among the three Kl. lactisstrains. The inhibitory effect of the tested yeasts was different according to the applied mould strain temperature, culture medium and cell density. Application of antagonistic yeasts combined with reduced temperature enhanced the inhibitory effect. Direct relationship was observed between increasing cell density and the biocontrol efficiency of Kl. lactis. According to this work, Kl. lactisis a possible biocontrol agent.
Fan, Q. & Tian, S. (2001): Postharvest biological control of grey mould and blue mould on apple by Cryptococcus albidus (Saito) Skinner. Postharvest Biol. Technol., 21 341-350.
'Postharvest biological control of grey mould and blue mould on apple by Cryptococcus albidus (Saito) Skinner ' () 21 Postharvest Biol. Technol. : 341 -350.
Janisiewicz, W.J., Peterson, D.L. & Bors, R. (1994): Control of storage decay of apples with Sporobolomyces roseus. Plant Disease, 78, 466-470.
'Control of storage decay of apples with Sporobolomyces roseus ' () 78 Plant Disease : 466 -470.
Janisiewicz, W.J., Tworkoski, T.J. & Kurtzman, C.P. (2001): Biocontrol potential of Metschnikowia pulcherrima strains against blue mould of apple. Phytopathology, 91, 1098-1108.
'Biocontrol potential of Metschnikowia pulcherrima strains against blue mould of apple ' () 91 Phytopathology : 1098 -1108.
Jijakli, M. & Lepoivre, P. (1998): Characterization of an exo-β-1,3-glucanase produced by Pichia anomala strain K, antagonist of Botrytis cinerea on apples. Phytopathology, 88, 335-343.
'Characterization of an exo-β-1,3-glucanase produced by Pichia anomala strain K, antagonist of Botrytis cinerea on apples ' () 88 Phytopathology : 335 -343.
Karbulut, O.A., Cohen, L., Wiess, B., Daus, A., Lurie, S. & Droby, S. (2002): Control of brown rot and blue mould of peach and nectarine by short hot water brushing and yeast antagonists. Postharvest Biol. Technol., 24, 103-111.
'Control of brown rot and blue mould of peach and nectarine by short hot water brushing and yeast antagonists ' () 24 Postharvest Biol. Technol. : 103 -111.
Lund, B.M. & Snowdon, A.L. (2000): Fresh and processed fruits. -in: Lund, B.M., Baird-Parker, T.C., Gould, G.W. (Eds) The microbiological safety and quality of food. Aspen Publishers, Inc., Gaithersburg. pp. 738-758.
Fresh and processed fruits , () 738 -758.
Petersson, S. & Schnürer, J. (1995): Biocontrol of mould growth in high-moisture wheat stored under airtight conditions by Pichia anomala, Pichia guilliermondii and Saccharomyces cerevisiae. Appl. environ. Microbiol., 61, 1027-1032.
'Biocontrol of mould growth in high-moisture wheat stored under airtight conditions by Pichia anomala, Pichia guilliermondii and Saccharomyces cerevisiae ' () 61 Appl. environ. Microbiol. : 1027 -1032.
Björnberg, A. & Schnürer, J. (1993): Inhibition of growth of grain-storage moulds in vitro by the yeast Pichia anomala (Hansen) Kurtzman. Can. J. Microbiol. 39, 623-628.
'Inhibition of growth of grain-storage moulds in vitro by the yeast Pichia anomala (Hansen) Kurtzman ' () 39 Can. J. Microbiol. : 623 -628.
Dock, L.L., Nielsen, P.V. & Floros, J.D. (1998): Biological control of Botrytis cinerea growth on apples stored under modified atmospheres. J. Fd. Prot. 61, 1661-1665.
'Biological control of Botrytis cinerea growth on apples stored under modified atmospheres ' () 61 J. Fd. Prot. : 1661 -1665.
Sommer, N.F. (1985): Principles of disease suppression by handling practises. -in: Kader, A.A., Kasmire, R.F., Mitchell, F.G., Reid, M.S., Sommer, N.F. & Thompson, J.F. Postharvest technology of horticultural crops. University of California. Division of Agriculture and Natural Resources. pp. 75-82.
Principles of disease suppression by handling practises , () 75 -82.
Spadaro, D., Vola, R., Piano, S. & Gullino, M.L. (2002): Mechanism of action and efficacy of four isolates of the yeast Metschnikowia pulcherrima active against postharvest pathogens on apples. Postharvest Biol. Technol., 24, 123-134.
'Mechanism of action and efficacy of four isolates of the yeast Metschnikowia pulcherrima active against postharvest pathogens on apples ' () 24 Postharvest Biol. Technol. : 123 -134.
Petersson, S. & Schnürer, J. (1998): Pichia anomala as a biocontrol agent of Penicillium roqueforti in high-moisture wheat, rye, barley, and oats stored under airtight conditions. Can. J. Microbiol., 44, 471-476.
'Pichia anomala as a biocontrol agent of Penicillium roqueforti in high-moisture wheat, rye, barley, and oats stored under airtight conditions ' () 44 Can. J. Microbiol. : 471 -476.
Porat, R., Daus, A., Weiss, B., Cohen, L., Fallik, E. & Droby, S. (2000): Reduction of postharvest decay in organic citrus fruit by short hot water brushing treatment. Postharvest Biol. Technol., 18, 151-157.
'Reduction of postharvest decay in organic citrus fruit by short hot water brushing treatment ' () 18 Postharvest Biol. Technol. : 151 -157.
Yackel, W.C., Nelson, A.I., Wei, L.S. & Steinberg, M.P. (1971): Effect of controlled atmosphere on growth of mould on synthetic media and fruit. Appl. Microbiol., 22, 513-516.
'Effect of controlled atmosphere on growth of mould on synthetic media and fruit ' () 22 Appl. Microbiol. : 513 -516.
Usall, J., Teixidó, N., Torres, R., De Eribe, X.O. & Viñas, I. (2001): Pilot test of Candida sake (CPA-1) applications to control postharvest blue mould on apple fruit. Postharvest Biol. Technol., 21, 147-156.
'Pilot test of Candida sake (CPA-1) applications to control postharvest blue mould on apple fruit ' () 21 Postharvest Biol. Technol. : 147 -156.
Vero, S., Mondino, P., Burgueño, J., Soubes, M. & Wisniewski, M. (2002): Characterization of biocontrol activity of two yeast strains from Uruguay against blue mould of apple. Postharvest Biol. Technol., 26, 91-98.
'Characterization of biocontrol activity of two yeast strains from Uruguay against blue mould of apple ' () 26 Postharvest Biol. Technol. : 91 -98.