In vitro investigations for the antioxidant and free radical scavenging activity of Myrtilli folium-, Phaseoli fructus sine seminibus- and a drug mixture (Equiseti herba, Myrtilli folium, Phaseoli fructus sine seminibus, Urticae folium) extracts showed antioxidant (LPO inhibitory and chain-breaking antioxidant) activity and free radical (superoxide anion and hydroxyl radical) scavenging effect. The extracts inhibited lipid peroxidation induced enzymatically by adding NADPH and non-enzymatically by adding Fe2+ in brain microsomes and in brain homogenates, respectively. The extracts reduced the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), which showed chain-breaking antioxidant activity. The extracts scavenged superoxide radicals (O2-·) by inhibiting the reduction of nitro blue tetrazolium evoked by phenazine methosulphate. In addition, the extracts inhibited Fenton-reagent (Fe2+ and H2O2) induced deoxyribose degradation, therefore, it was concluded that the extracts have hydroxyl radical (OH·) scavenging property.
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Catella-Lawson, F. & FitzGerald, G. (1996): Oxidative stress and platelet activation in diabetes mellitus. Diabetes Res. clin. Pract, 30, S13-S18.
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Bertuglia, S., Malandrino, S. & Colantuoni, A. (1995): Effects of the natural flavonoid delphinidin on diabetic microangiopathy. Arzneim.-Forsch., 45, 481-485.
'Effects of the natural flavonoid delphinidin on diabetic microangiopathy. ' () 45 Arzneim.-Forsch. : 481 -485 .
Czinner, E., Hagymási, K., Blázovics, A., Kéry, Á., Szöke, É. & Lemberkovics, É. (2001): The in vitro effect of Helichrysi flos on microsomal lipid peroxidation. J. Ethnopharmac, 77, 31-35.
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DAB (1999): Deutsches Arzneibuch Ed. 10, Deutscher Apotheker Verlag, Stuttgart.
Duke, J. A. (2000): CRC Handbook of medicinal herbs. CRC Press, Inc., Boca Raton, Florida, pp. 1-677.
1 677
Ernst, E. (1997): Plants with hypoglycemic activity in humans. Phytomedicine, 4, 73-78.
'Plants with hypoglycemic activity in humans. ' () 4 Phytomedicine : 73 -78 .
European Pharmacopoeia (2002): Thin layer chromatography. Ed. 4. 2.2.27. Strasbourg, Council of Europe.