Author: J. Szamos 1
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  • 1 Department of Nutrition, Central Food Research Institute H-1022 Budapest, Herman Ottó út 15. Hungary
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In three-phase partitioning (TPP) ammonium sulphate and 2-methyl-2-propanol (tert-butanol) are used for separation of proteins by collecting and concentrating them into the middle layer or third phase of the system. During the last two decades, TPP has been primarily applied to enzyme purification at laboratory scale, however, its analytical type application for investigating meat has also been reported. Here, an experimental set of TPP feasible to analytical scale experiments is described to study partitioning behaviour of carp and corn proteins. In partitioning experiments, corn proteins extractable with borate buffer, and sarcoplasmic proteins of carp were investigated. In the pH-range of 4.8-7.9, composition of partitioned corn proteins showed independence of pH as it was indicated by nonequilibrium pH gradient electrophoresis (NEpHGE) patterns. In case of carp, the alterations of protein composition in the pH-range of 3.7-8.0 unambiguously indicated the pH-dependent partitioning of carp proteins. The analytical scale method, based on three-phase partitioning is a new approach to investigate food proteins.

  • A.O.A.C. (1996): Fish and other marine products 937.07

  • Hamaker, B.R., Mohamed, A.A., Habben, J.E., Huang, C.P. & Larkins, B.A. (1995): Efficient procedure for extracting maize and sorghum kernel proteins reveals higher prolamin contents than the conventional method. Cereal Chem., 72, 583-588.

    'Efficient procedure for extracting maize and sorghum kernel proteins reveals higher prolamin contents than the conventional method ' () 72 Cereal Chem. : 583 -588.

    • Search Google Scholar
  • Janosi, A. & Szamos, J. (2001): Comparison of two methods in purification of meat-DNA for PCR. Acta Alimentaria, 30, 113-118.

    'Comparison of two methods in purification of meat-DNA for PCR ' () 30 Acta Alimentaria : 113 -118.

    • Search Google Scholar
  • Kocher, P.N. & Foegeding, E.A. (1993): Microcentrifuge-based method for measuring water-holding of protein gels. J. Fd Sci., 58, 1040-1046.

    'Microcentrifuge-based method for measuring water-holding of protein gels ' () 58 J. Fd Sci. : 1040 -1046.

    • Search Google Scholar
  • Neuhoff, V., Arold, N., Taube, D. & Ehrhardt, W. (1988): Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis, 9, 255/262.

    'Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250 ' () 9 Electrophoresis : 255 -262.

    • Search Google Scholar
  • Odegaard, B.H., Anderson, P.C. & Lovrien, R.E. (1984): Resolution of the multienzynie cellulase complex of Trichoderma reesei QM9414. J. appl. Biochem., 6, 156-183.

    'Resolution of the multienzynie cellulase complex of Trichoderma reesei QM9414 ' () 6 J. appl. Biochem. : 156 -183.

    • Search Google Scholar
  • Penny, I.F. (1975): Use of a centrifuging method to measure the drip of pork Longissimus dorsi slices before and after freezing and thawing. J. Sci. Fd Agric., 26, 1593-1602.

    'Use of a centrifuging method to measure the drip of pork Longissimus dorsi slices before and after freezing and thawing ' () 26 J. Sci. Fd Agric. : 1593 -1602.

    • Search Google Scholar
  • Pike, R.N. & Dennison, C. (1989): Protein fractionation by three phase partitioning (TPP) in aqueous/t/butanol mixtures. Biotechnol Bioengng., 33, 221-228.

    'Protein fractionation by three phase partitioning (TPP) in aqueous/t/butanol mixtures ' () 33 Biotechnol Bioengng. : 221 -228.

    • Search Google Scholar
  • Sharma, A. & Gupta, M.N. (2001): Three phase partitioning as a large-scale separation method for purification of a wheat germ bifunctional protease/amylase inhibitor. Process Biochem., 37, 193-196.

    'Three phase partitioning as a large-scale separation method for purification of a wheat germ bifunctional protease/amylase inhibitor ' () 37 Process Biochem. : 193 -196.

    • Search Google Scholar
  • Szamos, J. & Hoschke, Á. (1992): Purification of horseradish peroxidase by three-phase partitioning. Acta Alimentaria, 21, 253-260.

    'Purification of horseradish peroxidase by three-phase partitioning ' () 21 Acta Alimentaria : 253 -260.

    • Search Google Scholar
  • Szamos, J., JÁnosi, A., Tamás, B. & Kiss, É. (1998): A novel partitioning method as a possible tool for investigating meat. I. Z. Lebensmittelunters. Forsch. A, 206, 208-212.

    'A novel partitioning method as a possible tool for investigating meat. I. Z ' () 206 Lebensmittelunters. Forsch. : 208 -212.

    • Search Google Scholar
  • Dennison, C. & Lovrien, R. (1997): Three phase partitioning: concentration and purification of proteins. Protein Expression Purification, 11, 149-161.

    'Three phase partitioning: concentration and purification of proteins ' () 11 Protein Expression Purification : 149 -161.

    • Search Google Scholar

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