The efficiency of seven disinfectants (Divomil Forte, Nobactel, Domestos, SU 392, Buraton, Descosal, Cidex) was tested against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC 10231, Bacillus subtilis ATCC 6633, Aspergillus niger ATCC 16404. Surface test was used in order to evaluate the efficiency of disinfectants during the everyday sanitizing practice on special industrial surfaces. Test organisms represented pathogenic, spore forming bacteria, yeast and mould. Surface test was started with minimal concentration of agents and was not increased above the maximal concentrations, which were recommended by manufacturers in order not to corrode surfaces or risk the safety of use. Test organisms were inoculated on test areas and after drying inoculated surfaces were treated with disinfectants. Seven disinfectants were tested and four were effective against every test organism. (Buraton: 1%, Descosal: 1%, Cidex: 100%, SU 392: 75%.) Two disinfectants were ineffective against Aspergillus niger (Nobactel: 2%, Domestos: 2%) and 1 against Staphylococcus aureus and Aspergillus niger (Divomil Forte: 2%).