Inactivation and removing efficiency of disinfectants (Descosal and Domestos) was tested against Pseudomonas aeruginosa and Candida albicans and their biofilms (2 and 72 h, 3, 7 and 14 days old, respectively). The survival of microbes was studied by pour plating and impedimetry, and the attachment by epifluorescent microscopy.In the surface test, both disinfectants were effective against both test organisms with no significant difference in their efficiency. The decreasing detection times during impedimetric measurements showed higher resistance of the older Ps. aeruginosa biofilms. Both disinfectants tested were fungicidal against C. albicans and bactericidal against Ps. aeruginosa but for biofilm removal Domestos was more efficient.