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  • 1 University of Maribor Faculty of Agriculture Vrbanska 30 SI-2000 Maribor Slovenia
  • | 2 Agricultural Institute of Slovenia Hacquetova 17 SI-1001 Ljubljana Slovenia
  • | 3 University of Maribor Medical Faculty Slomškov trg 15 SI-2000 Maribor Slovenia
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The influence of three milling techniques (MT1: industrial roller-grinder, MT2: grain hammer crasher, and MT3: traditional millstone) and two baking methods (BM1: industrial oven, BM2: traditional ceramic stove heated by wood (log fire oven)) on mycotoxin deoxynivalenol (DON) and nivalenol (NIV) levels in bread were investigated. The DON and NIV concentrations in wheat grain, flour, and bread were analysed using high performance liquid chromatography with UV-detection methods. The 2 500 kg lot of wheat grain containing 1 400–1 900 μg kg −1 deoxynivalenol and 130–200 μg kg −1 nivalenol was divided into sub-lots which were processed to get three types of flour (F1: industrial bread flour, F2: industrial wholegrain flour and F3: traditional wholegrain flour). The concentrations of DON and NIV measured after milling the grain according to MT1 (yielding F1) amounted to 310–370 \g kg −1 and <50–70 μg kg t1 , respectively. After applying MT2 to the grain (yielding F2), the DON and NIV levels were measured to be 1 060–1 400 μg kg −1 and 60–87 μg kg −1 , respectively. Applying MT3 (yielding F3) produced a DON level of 1 100–1 770 μg kg 1 and a NIV level of 80–95 μg kg −1 . Six types of bread were baked from the three types of flour according to BM1 or BM2, and the mycotoxin levels were analysed. The average reduction in DON concentration after baking (70 min at 195–235 °C) was 47.2% for bread baked in the industrial oven and 48.7% for bread baked in the log fire oven. Concentrations of DON in bread prepared by the industrial MT1 were under the permitted limit of 500 μg kg −1 stated in EC (2006) regulation, despite the fact that the bread was baked from grains highly contaminated with mycotoxins. In the bread baked from traditional wholegrain flour, mycotoxin concentrations were higher (850–950 μg kg −1 ).

  • Abbas, H.K., Mirocha, C.J., Pawlovsky, R.J. & Pusch, D.J. (1985): Effect of cleaning, milling, and baking on deoxynivalenol in wheat. Appl. environ. Microbiol. , 50 , 482–486.

    Pusch D.J. , 'Effect of cleaning, milling, and baking on deoxynivalenol in wheat ' (1985 ) 50 Appl. environ. Microbiol. : 482 -486.

    • Search Google Scholar
  • Bennet, G.A. & Richard, J.L. (1996): Influence of processing on Fusarium mycotoxins in contaminated grains. Fd Technol., 50 , 235–239.

    Richard J.L. , 'Influence of processing on Fusarium mycotoxins in contaminated grains ' (1996 ) 50 Fd Technol. : 235 -239.

    • Search Google Scholar
  • Birzele, B., Meier, A., Hindorf, H., Kramer, J. & Dehne, H.W. (2002): Epidemiology of Fusarium infection and deoxynivalenol content in winter wheat in the Rhineland, Germany. Eur. J. Pl. Pathol. , 108 , 667–673.

    Dehne H.W. , 'Epidemiology of Fusarium infection and deoxynivalenol content in winter wheat in the Rhineland, Germany ' (2002 ) 108 Eur. J. Pl. Pathol. : 667 -673.

    • Search Google Scholar
  • Bretz, M., Beyer, M., Cramer, B., Knecht, A. & Humpf, H.U. (2006): Thermal degradation of the Fusarium mycotoxin deoxynivalenol. J. agric. Fd Chem. , 54 , 6445–6451.

    Humpf H.U. , 'Thermal degradation of the Fusarium mycotoxin deoxynivalenol ' (2006 ) 54 J. agric. Fd Chem. : 6445 -6451.

    • Search Google Scholar
  • Cirillo, T., Ritieni, A., Visone, M. & AMODIO COCCHIERI , R. (2003a): Evaluation of conventional and organic Italian foodstuffs for deoxynivalenol and fumonisins B1 and B2. J. agric. Fd Chem. , 51 , 8128–8131.

    Amodio Cocchieri R. , 'Evaluation of conventional and organic Italian foodstuffs for deoxynivalenol and fumonisins B1 and B2 ' (2003 ) 51 J. agric. Fd Chem. : 8128 -8131.

    • Search Google Scholar
  • Cirillo, T., Ritieni, A., Galvano, F. & AMODIO COCCHIERI , R. (2003b): Natural co-occurrence of deoxynivalenol and fumonisins B1 and B2 in Italian marketed foodstuffs. Fd Addit. Contam. , 20 , 566–571.

    Amodio Cocchieri R. , 'Natural co-occurrence of deoxynivalenol and fumonisins B1 and B2 in Italian marketed foodstuffs ' (2003 ) 20 Fd Addit. Contam. : 566 -571.

    • Search Google Scholar
  • Chelkowski, J. & Perkowski, J. (1992): Mycotoxins in cereal grains’ Distribution of deoxynivalenol in naturally contaminated wheat kernels. Mycotoxin Res. , 8 , 27–30.

    Perkowski J. , 'Mycotoxins in cereal grains’ Distribution of deoxynivalenol in naturally contaminated wheat kernels ' (1992 ) 8 Mycotoxin Res. : 27 -30.

    • Search Google Scholar
  • EC (2005): Commission regulation No. 856/2005 of 6 June 2005 amending Regulation (EC) No 466/2001 as regards Fusarium toxins . Off. J. European Union , L 143/3–143/8.

  • EC (2006): Commission regulation No. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off. J. European Union , L 364/5–364/24.

  • FAO (2002): Discussion paper on deoxynivalenol . Codex Alimentarius Commission, Codex Committee of Food Additives and Contaminants, 35th Session, Arusha, Tanzania, 17–21 March, pp. 1–12.

  • Fuchs, E., Handel, J. & Binder, E.M. (2004): LC-MS/LC-UV analysis of type-A and-B trichothecenes after multifunctional MycoSep® clean-up.-in: Yoshizava, T. (Ed) Proceedings of ISMYCO Conference. New horizons of mycotoxicology for assuring food safety . Kagawa, Japan, 2003, pp. 225–232.

  • Harcz, P. , De Temmerman, L. , De Voghel, S., Waegeneers, N., Wilmart, O., Vromman, V., Schmit, J.F., Moons, E. , Van Peteghem, C. , De Saeger, S., Schneider, Y.J. & Pussemier, L. (2007): Contaminants in organically and conventionally produced winter wheat ( Triticum aestivum ) in Belgium. Fd Addit. Contam., 24 , 713–720.

    Pussemier L. , 'Contaminants in organically and conventionally produced winter wheat (Triticum aestivum) in Belgium ' (2007 ) 24 Fd Addit. Contam. : 713 -720.

    • Search Google Scholar
  • Hazel, C.M. & Patel, S. (2004): Influence of processing on trichothecene levels. Toxicol. Lett. , 153 , 51–59.

    Patel S. , 'Influence of processing on trichothecene levels ' (2004 ) 153 Toxicol. Lett. : 51 -59.

    • Search Google Scholar
  • Larsen, J.C., Hunt, J., Perrin, I. & Ruckenbauer, P. (2004): Workshop on trichothecenes with a focus on DON: summary report. Toxicol. Lett. , 153 , 1–22.

    Ruckenbauer P. , 'Workshop on trichothecenes with a focus on DON: summary report ' (2004 ) 153 Toxicol. Lett. : 1 -22.

    • Search Google Scholar
  • Lauren, D.R. & Smith, W.A. (2001): Stability of the Fusarium mycotoxins nivalenol, deoxynivalenol and zearalenon in ground maize under typical cooking environments. Fd Addit. Contam. , 18 , 1011–1016.

    Smith W.A. , 'Stability of the Fusarium mycotoxins nivalenol, deoxynivalenol and zearalenon in ground maize under typical cooking environments ' (2001 ) 18 Fd Addit. Contam. : 1011 -1016.

    • Search Google Scholar
  • Magkos, F., Arvaniti, F. & Zampelas, A. (2006): Organic food: buying more safety or just peace of mind? A Critical review of the literature. Crit. Rev. Fd Sci. Nutr. , 46 , 23–56.

    Zampelas A. , 'Organic food: buying more safety or just peace of mind? A Critical review of the literature ' (2006 ) 46 Crit. Rev. Fd Sci. Nutr. : 23 -56.

    • Search Google Scholar
  • Malmauret, L., Parent-Massin, D., Hardy, J.L. & Verger, P. (2002): Contaminants in organic and conventional foodstuffs in France. Fd Addit. Contam. , 19 , 524–532.

    Verger P. , 'Contaminants in organic and conventional foodstuffs in France ' (2002 ) 19 Fd Addit. Contam. : 524 -532.

    • Search Google Scholar
  • Murphy, P.A., Hendrich, S., Landgren, C. & Bryant, C.M. (2006): Food mycotoxins: An update (Scientific status summary). J. Fd Sci. , 71 , 51–65.

    Bryant C.M. , 'Food mycotoxins: An update (Scientific status summary) ' (2006 ) 71 J. Fd Sci. : 51 -65.

    • Search Google Scholar
  • Nowicki, T.W., Gaba, D.G., Dexter, J.E., Matsuo, R.R. & Clear, M.R. (1988): Retention of DON in wheat during processing and cooking of spaghetti and noodles. J. Cereal Sci. , 8 , 189–202.

    Clear M.R. , 'Retention of DON in wheat during processing and cooking of spaghetti and noodles ' (1988 ) 8 J. Cereal Sci. : 189 -202.

    • Search Google Scholar
  • Samar, M.M., Ferro-Fontan, C., Resnik, S.L. & Pacin, A.M. (2001): Effect of fermentation on naturally occurring DON in Argentinean bread processing technology. Fd Addit. Contam. , 18 , 1004–1010.

    Pacin A.M. , 'Effect of fermentation on naturally occurring DON in Argentinean bread processing technology ' (2001 ) 18 Fd Addit. Contam. : 1004 -1010.

    • Search Google Scholar
  • Samar, M.M., Ferro-Fontan, C., Resnik, S.L., Pacin, A.M. & Castillo, M.D. (2003): Distribution of deoxynivalenol in wheat, wheat flour, bran, and gluten and variability associated with the test procedure. J. AOC int. , 86 , 551–556.

    Castillo M.D. , 'Distribution of deoxynivalenol in wheat, wheat flour, bran, and gluten and variability associated with the test procedure ' (2003 ) 86 J. AOC int. : 551 -556.

    • Search Google Scholar
  • Schollenberger, M., Suchy, S., Jara, H.T., Drochner, W. & Müller, H.M. (2002): A survey of Fusarium toxins in cereal-based foods marketed in an area of southwest Germany. Int. J. Fd Microbiol. , 72 , 85–89.

    Müller H.M. , 'A survey of Fusarium toxins in cereal-based foods marketed in an area of southwest Germany ' (2002 ) 72 Int. J. Fd Microbiol. : 85 -89.

    • Search Google Scholar
  • Schollenberger, M., Drochner, W., Rüfle, M., Jara, H.T. & Müller, H.M. (2005): Trichothecene toxins in different groups of conventional and organic bread of the German market. J. Fd Compos. Anal. , 18 , 69–78.

    Müller H.M. , 'Trichothecene toxins in different groups of conventional and organic bread of the German market ' (2005 ) 18 J. Fd Compos. Anal. : 69 -78.

    • Search Google Scholar
  • Scott, P.M., Kanhere, S.R., Lau, P.Y. & Dexter, J.E. (1983): Effects of experimental flour milling and bread baking on retention of deoxynivalenol (vomitoxin) in hard red spring wheat. Cereal Chem. , 60 , 421–424.

    Dexter J.E. , 'Effects of experimental flour milling and bread baking on retention of deoxynivalenol (vomitoxin) in hard red spring wheat ' (1983 ) 60 Cereal Chem. : 421 -424.

    • Search Google Scholar
  • Scott, P.M., Kanhere, S.R., Dexter, J.E., Brennan, P.W. & Trenhom, H.L. (1984): Distribution of the trichothecene mycotoxin Deoxynivalenol (Vomitoxin) during the milling of naturally contaminated hard red spring wheat and its fate in baked products. Fd Addit. Contam. , 1 , 313–322.

    Trenhom H.L. , 'Distribution of the trichothecene mycotoxin Deoxynivalenol (Vomitoxin) during the milling of naturally contaminated hard red spring wheat and its fate in baked products ' (1984 ) 1 Fd Addit. Contam. : 313 -322.

    • Search Google Scholar
  • Seitz, L.M., Eustace, W.D., Mohr, H.E., Shogren, M.D. & Yamazaki, V.T. (1986): Cleaning, milling, and baking tests with hard red winter wheat containing deoxynivalenol. Cereal Chem. , 63 , 146–150.

    Yamazaki V.T. , 'Cleaning, milling, and baking tests with hard red winter wheat containing deoxynivalenol ' (1986 ) 63 Cereal Chem. : 146 -150.

    • Search Google Scholar
  • Sugita-Konishi, Y., Park, B.J., Kobayashi-Hattori, K., Tanaka, T., Yoshikawa, K. & Kumagai, S. (2006): Effect of cooking process on the deoxynivalenol content and its subsequent cytotoxicity in wheat products. Bioscience Biotechnol. Biochem. , 70 , 1764–1768.

    Kumagai S. , 'Effect of cooking process on the deoxynivalenol content and its subsequent cytotoxicity in wheat products ' (2006 ) 70 Bioscience Biotechnol. Biochem. : 1764 -1768.

    • Search Google Scholar
  • Tkachuk, R., Dexter, J.E., Tipples, K.H. & Nowicki, T.W. (1991): Removal by specific gravity table of tombstone kernels and associated trichothecene from wheat infected with Fusarium head blight. Cereal Chem. , 68 , 428–431.

    Nowicki T.W. , 'Removal by specific gravity table of tombstone kernels and associated trichothecene from wheat infected with Fusarium head blight ' (1991 ) 68 Cereal Chem. : 428 -431.

    • Search Google Scholar
  • Trigo-Stockli, D.M., Deyoe, C.W., Satumbaga, R.F. & Pedersen, J.R. (1996): Distribution of deoxynivalenol and zearalenon in milled fractions of wheat. Cereal Chem. , 73 , 388–391.

    Pedersen J.R. , 'Distribution of deoxynivalenol and zearalenon in milled fractions of wheat ' (1996 ) 73 Cereal Chem. : 388 -391.

    • Search Google Scholar
  • Visconti, A., Haidukowski, E.M., Pascale, M. & Silvestri, M. (2004): Reduction of deoxynivalenol during durum wheat processing and spaghetti cooking. Toxicol. Lett. , 153 , 181–189.

    Silvestri M. , 'Reduction of deoxynivalenol during durum wheat processing and spaghetti cooking ' (2004 ) 153 Toxicol. Lett. : 181 -189.

    • Search Google Scholar
  • Wolf-Hall, C., Hanna, M. & Bullerman, L. (1999): Stability of deoxynivalenol in heat-treated foods. J. Fd Prot. , 62 , 962–964.

    Bullerman L. , 'Stability of deoxynivalenol in heat-treated foods ' (1999 ) 62 J. Fd Prot. : 962 -964.

    • Search Google Scholar
  • Young, J.C., Fulcher, R.G., Hayhoe, J.H., Scott, P.M. & Dexter, J.E. (1984): Effect of milling and baking on deoxynivalenol (vomitoxin) content of eastern Canadian wheats. J. agric. Fd Chem. , 32 , 659–664.

    Dexter J.E. , 'Effect of milling and baking on deoxynivalenol (vomitoxin) content of eastern Canadian wheats ' (1984 ) 32 J. agric. Fd Chem. : 659 -664.

    • Search Google Scholar
  • Yumbe-Guevera, B.E., Imoto, T. & Yoshizava, T. (2003): Effects of heating procedures on deoxynivalenol, nivalenol and zearalenon levels in naturally contaminated barley and wheat. Fd Addit. Contam. , 20 , 1132–1140.

    Yoshizava T. , 'Effects of heating procedures on deoxynivalenol, nivalenol and zearalenon levels in naturally contaminated barley and wheat ' (2003 ) 20 Fd Addit. Contam. : 1132 -1140.

    • Search Google Scholar
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