View More View Less
  • 1 University of Molise Department of Agricultural, Food, Environmental and Microbiological Science and Technologies (DiSTAAM) via De Sanctis 86100 Campobasso Italy
  • | 2 Indian Institute of Technology, Kharagapur Natural Product Laboratories, Chemistry Department 721 302 West Bengal India
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

In this laboratory research, we produced and compared different phenol extracts from olive-oil mill wastewaters. The extracts and sample preparation was as follows: 1 CW-Acetone (wastewater, previously centrifuged and treated with acetone to precipitate colloid substances); 2 CW-Ac-HCl-EtAc (this extract was recovered by ethyl acetate from the 1 CW-Acetone prior to being hydrolysed by 1N HCL at 80 °C for 1 h); 3.2 CW-20% MetOH and 4.2 CW-80% MetOH (fractions separated by solid-liquid extraction (SPE) with methanol:water mixtures at 20% and 80% (v/v), respectively).Determined in each sample were: (i) total phenols and ortho -di-phenol conducive to spectrophotometric methods; (ii) phenol composition by HPLC analysis and finally, (iii) antioxidant activity on a lard sample, using rancimat test under 120 °C temperature and 20 l h −1 air flow. The extract 1 CW-Acetone, composed essentially of local wastewater phenols, was less effective than the other extracts. Best antioxidant extract was 3.2 CW-20% MetOH that prolonged the lard’s oxidative stability 3.5 and 7.0 times at doses of 100 and 200 mg kg −1 , respectively. Antioxidant effectiveness of the various extracts were found to be directly correlating with percentage of free hydroxytyrosol. Finally, antioxidant properties of olive-oil mill wastewater extract samples was found to be the result of their phenol composition rather than their phenol content.

  • Agalias, A. , Magiatis, P. , Skaòtsounis, A.L. , Mikros, E. , Tsarbopoulos, A. , Gikas, E. , Spanos, I. & Manios, T. (2007): A new process for the management of olive oil mill waste water and recovery of natural antioxidants. J. agric. Fd Chem. , 55 , 2671–2676.

    Manios T. , 'A new process for the management of olive oil mill waste water and recovery of natural antioxidants ' (2007 ) 55 J. agric. Fd Chem. : 2671 -2676.

    • Search Google Scholar
  • Bianchi, A. & Pozzi, N. (1994): 3,4-Dihydroxyphenylglycol, a major C6-C2 phenolic in Olea europaea fruits. Phytochemistry , 35 , 1335–1337.

    Pozzi N. , '3,4-Dihydroxyphenylglycol, a major C6-C2 phenolic in Olea europaea fruits ' (1994 ) 35 Phytochemistry : 1335 -1337.

    • Search Google Scholar
  • Bisignano, G. , Tomaino, A. , Lo Cascio, R. , Crisafi, G. , Uccella, N. & Saija, A. (1999): On the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol. J. Pharmacy Pharmacol , 51 , 971–974.

    Saija A. , 'On the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol ' (1999 ) 51 J. Pharmacy Pharmacol : 971 -974.

    • Search Google Scholar
  • Capasso, R. , Evidente, A. , Avolio, S. & Solla, F. (1999): A highly convenient synthesis of hydroxytyrosol and its recovery from agricultural waste waters. J. agric. Fd Chem. , 47 , 1745–1748.

    Solla F. , 'A highly convenient synthesis of hydroxytyrosol and its recovery from agricultural waste waters ' (1999 ) 47 J. agric. Fd Chem. : 1745 -1748.

    • Search Google Scholar
  • Chimi, H. , Rahmani, M. , Cillard, J. & Cillard, P. (1990): Autooxidation des huiles d’olive: rôle des composés phénoliques. Rev. Fr. Corps Gras , 37 , 363–367.

    Cillard P. , 'Autooxidation des huiles d’olive: rôle des composés phénoliques ' (1990 ) 37 Rev. Fr. Corps Gras : 363 -367.

    • Search Google Scholar
  • De Leonardis, A. , Macciola, V. , Lembo, G. , Aretini, A. & Nag, A. (2007a): Studies on oxidative stabilisation of lard by natural antioxidants recovered from olive-oil mill wastewater. Fd Chem. , 100 , 998–1004.

    Nag A. , 'Studies on oxidative stabilisation of lard by natural antioxidants recovered from olive-oil mill wastewater ' (2007 ) 100 Fd Chem. : 998 -1004.

    • Search Google Scholar
  • De Leonardis, A. , Aretini, A. , Alfano, G. , Macciola, V. & Ranalli, G. (2007b): Isolation of a hydroxytyrosol-rich extract from olive leaves ( Olea europaea L.) and evaluation of its antioxidant properties and bioactivity. Eur. Fd Res. Technol. (press on line: DOI 10.1007/s00217-007-0574-3).

  • De Marco, E. , Savarese, M. , Paduano, A. & Sacchi, R. (2007): Characterization and fraction of phenolic compounds extracted from olive oil mill wastewaters. Fd Chem. , 104 , 858–867.

    Sacchi R. , 'Characterization and fraction of phenolic compounds extracted from olive oil mill wastewaters ' (2007 ) 104 Fd Chem. : 858 -867.

    • Search Google Scholar
  • Fernández-Bolaňos, J. , Rodríguez, G. , Rodríguez, R. , Heredia, A. , Guillén, R. & Jiménez, A. (2002): Production in large quantities of highly purified hydroxytyrosol from liquid solid waste of two-phase olive oil processing or “Alperujio”. J. agric. Fd Chem. , 50 , 6804–6811.

    Jiménez A. , 'Production in large quantities of highly purified hydroxytyrosol from liquid solid waste of two-phase olive oil processing or “Alperujio” ' (2002 ) 50 J. agric. Fd Chem. : 6804 -6811.

    • Search Google Scholar
  • Fki, I. , Allouche, N. & Sayadi, S. (2005): The use of polyphenolic extract, purified hydroxytyrosol and 3,4-dihydroxyphenyl acetic acid from olive mill wastewaters for the stabilization of refined oils: a potential alternative to synthetic antioxidants. Fd Chem. , 93 , 197–204.

    Sayadi S. , 'The use of polyphenolic extract, purified hydroxytyrosol and 3,4-dihydroxyphenyl acetic acid from olive mill wastewaters for the stabilization of refined oils: a potential alternative to synthetic antioxidants ' (2005 ) 93 Fd Chem. : 197 -204.

    • Search Google Scholar
  • Folin, O. & Ciocalteau, V. (1927): On tyrosine and tryptophan determination in protein. J. biol. Chem. , 73 , 627–650.

    Ciocalteau V. , 'On tyrosine and tryptophan determination in protein ' (1927 ) 73 J. biol. Chem. : 627 -650.

    • Search Google Scholar
  • Frega, N. , Mozzon, M. , Servidio, G. & Lercker, G. (1995): Studio della resistenza all’ossidazione forzata degli oli extra vergini mediante Rancimat test. (A study by using Rancimat test on controlled oxidation of extra-virgin olive oil.) Riv. Ital. Sostanze Grasse , 72 , 493–496.

    Lercker G. , 'Studio della resistenza all’ossidazione forzata degli oli extra vergini mediante Rancimat test ' (1995 ) 72 Riv. Ital. Sostanze Grasse : 493 -496.

    • Search Google Scholar
  • Gariboldi, P. , Jommi, G. & Verrotta, L. (1986): Secoiridoids from Olea europeae . Phytochemistry , 25 , 865–869.

    Verrotta L. , 'Secoiridoids from Olea europeae ' (1986 ) 25 Phytochemistry : 865 -869.

  • Japon-Lujan, R. & De Castro, M.D.L. (2007): Static-dynamic superheated liquid extraction of hydroxytyrosol and other biophenols from alperujo (a semisolid residue of the olive oil industry). J. agric. Fd Chem. , 55 , 3629–3643.

    Castro M.D.L. , 'Static-dynamic superheated liquid extraction of hydroxytyrosol and other biophenols from alperujo (a semisolid residue of the olive oil industry) ' (2007 ) 55 J. agric. Fd Chem. : 3629 -3643.

    • Search Google Scholar
  • Macciola, V. & De Leonardis, A. (2006): Natura e proprietà antiossidanti di fenoli estratti da un olio extra-vergine di oliva e suoi sottoprodotti. (Nature and antioxidant properties of phenolic extracts from an extra-virgin olive oil ( Olea europaea L.) and related by-products.) Ingredienti Alim. , 5 (6), 7–14.

    Leonardis A. , 'Natura e proprietà antiossidanti di fenoli estratti da un olio extra-vergine di oliva e suoi sottoprodotti ' (2006 ) 5 Ingredienti Alim. : 7 -14.

    • Search Google Scholar
  • Medina, I. , Tombo, I. , Satue-Gracia, M.T. , German, J.B. & Frankel, E.N. (2002): Effects of natural phenolic compounds on the antioxidant activity of lactoferrin in Liposomes and oil-in-water emulsions. J. agric. Fd Chem. , 50 , 596–601.

    Frankel E.N. , 'Effects of natural phenolic compounds on the antioxidant activity of lactoferrin in Liposomes and oil-in-water emulsions ' (2002 ) 50 J. agric. Fd Chem. : 596 -601.

    • Search Google Scholar
  • Niaounakis, M. & Halvadakis, C.P. (2004): Olive-mill waste management literature review and patent survey . Typothito-George Dardanos, Atene, Greek, pp. 2–30.

    Halvadakis C.P. , '', in Olive-mill waste management literature review and patent survey , (2004 ) -.

  • Paronetto, L. (1979): Polifenoli e tecniche enologiche . (Polyphenols and enologic techniques.) Edagricole, Bologna. Italy, pp. 125–130.

    Paronetto L. , '', in Polifenoli e tecniche enologiche , (1979 ) -.

  • Rodis, P.S. , Karathanos, V.T. & Mantzavinou, A. (2002): Partitioning of olive oil antioxidants between oil and water phases. J. agric. Fd Chem. , 50 , 596–601.

    Mantzavinou A. , 'Partitioning of olive oil antioxidants between oil and water phases ' (2002 ) 50 J. agric. Fd Chem. : 596 -601.

    • Search Google Scholar
  • Servili, M. , Baldioli, M. , Selvaggini, R. , Miniati, E. , Maccioni, A. & Montedoro, G. (1999): HPLC evaluation of phenols in olive fruit, virgin olive oil, vegetation waters and pomace, and 1D-and 2D-NMR characterization. J. Am. Oil Chem. Soc. , 76 , 873–882.

    Montedoro G. , 'HPLC evaluation of phenols in olive fruit, virgin olive oil, vegetation waters and pomace, and 1D-and 2D-NMR characterization ' (1999 ) 76 J. Am. Oil Chem. Soc. : 873 -882.

    • Search Google Scholar
  • Soler-Rivas, C. , Espín, J.C. & Wichers, H.J. (2000): Oleuropein and related compounds. J. Sci. Fd Agric. , 80 , 1013–1023.

    Wichers H.J. , 'Oleuropein and related compounds ' (2000 ) 80 J. Sci. Fd Agric. : 1013 -1023.

  • Tuck, K.L. & Hayball, P.J. (2002): Major phenolic compounds in olive oil: metabolism and health effects. J. Nutr. Biochem. , 13 , 636–644.

    Hayball P.J. , 'Major phenolic compounds in olive oil: metabolism and health effects ' (2002 ) 13 J. Nutr. Biochem. : 636 -644.

    • Search Google Scholar
  • Vásquez, R. , MAESTRO DURÁN , R. & Graciani, C. (1974): Componentes fenólicos de la aceituna. Polifenoles del alpechin. (Phenol components of olive oil mill waste water.) Grasas y aceites , 25 , 341–345.

    Graciani C. , 'Componentes fenólicos de la aceituna. Polifenoles del alpechin ' (1974 ) 25 Grasas y aceites : 341 -345.

    • Search Google Scholar
  • Visioli, F. & Galli, C. (1998): The effect of minor constituents of olive oil on cardiovascular disease: new finding. Nutr. Rev. , 56 , 142–147.

    Galli C. , 'The effect of minor constituents of olive oil on cardiovascular disease: new finding ' (1998 ) 56 Nutr. Rev. : 142 -147.

    • Search Google Scholar
  • Visioli, F. , Romani, A. , Mulinacci, N. , Zarini, S. , Conte, D. & Vincieri, F. (1999): Antioxidant and other biological activities in olive oil mill waste water. J. agric. Fd Chem. , 47 , 3397–3401.

    Vincieri F. , 'Antioxidant and other biological activities in olive oil mill waste water ' (1999 ) 47 J. agric. Fd Chem. : 3397 -3401.

    • Search Google Scholar
All Time Past Year Past 30 Days
Abstract Views 23 23 0
Full Text Views 9 8 0
PDF Downloads 2 1 0