To date, monosodium glutamate is the most used flavour enhancing food additive. Because high levels of glutamate are toxic to brain concerns appeared regarding the safe use of glutamate and there is a 10 g kg
concentration limit in foodstuff. A simple HPLC-UV method, based on a derivatization procedure with
-phthaldialdehyde, was developed for determination of glutamate in meat products, soup bases and vegetable concentrates. Even if our method is less sensitive than the HPLC-fluorescence ones widely available, it is able to measure amounts at least 200 times smaller than the maximum allowed one, has good reproducibility (CV under 2% for intraday and under 3% for interday determinations), linearity and accuracy. Less expensive HPLC systems are required and the formed derivative is very stable (at least 1 week), good separation is obtained with the less expensive 5 μm particle C
columns and methanol as organic phase. Concentration of free glutamate ranged between 0.14 g kg
in sausage without added glutamate to as high as 2.16 g kg
in a pork sausage. Concentration in vegetable mixes and soup bases were between 80–120 g kg
Effective determination of glutamic acid by HPLC with ELSD
. Varian Application Note SI-01214/TB1046.
Daniels, D.H., Joe, F.L. & Diachenko, G.W.
(1995): Determination of free glutamic acid in a variety of foods by high-performance liquid chromatography.
Fd Addit. Contam.
Diachenko G.W., 'Determination of free glutamic acid in a variety of foods by high-performance liquid chromatography' (1995) 12Fd Addit. Contam.: 21-29.
Diachenko G.W.Determination of free glutamic acid in a variety of foods by high-performance liquid chromatographyFd Addit. Contam.1995122129)| false
Ioannou, N., Georgopoulos, A., Liapi, C. & Palaiologos, G.
(1998): Determination of glutamate by high-performance liquid chromatography with fluorescence detection in superfusates of rat brain preparations.
Palaiologos G., 'Determination of glutamate by high-performance liquid chromatography with fluorescence detection in superfusates of rat brain preparations' (1998) 264Anal. Biochem.: 82-86.
Palaiologos G.Determination of glutamate by high-performance liquid chromatography with fluorescence detection in superfusates of rat brain preparationsAnal. Biochem.19982648286)| false