The isolation of verocytotoxin-producing Escherichia coli (VTEC) strains from improperly pasteurised cow’s milk samples

R. Kiss; G. Szita; M. Herpay; Gy. Csikó; J. Pászti; T. Mag; P. Kovács; G. Kovács; J. Szita; P. Tóth; I. Szatmári; S. Bernáth

doi:10.1556/aalim.2010.0013

Acta Alimentaria

Volume/Issue:: Volume 40: Issue 1

The isolation of verocytotoxin-producing Escherichia coli (VTEC) strains from improperly pasteurised cow’s milk samples

Authors:

R. Kiss

R. Kiss A.L. Instruments H-2112 Veresegyház Mester u. 9 Hungary

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G. Szita

G. Szita Szent István University Faculty of Veterinary Science H-1400 Budapest P.O. Box 2 Hungary

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M. Herpay

M. Herpay National Centre for Epidemiology H-1097 Budapest Gyáli út 2-6 Hungary

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Gy. Csikó

Gy. Csikó Szent István University Faculty of Veterinary Science H-1400 Budapest P.O. Box 2 Hungary

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J. Pászti

J. Pászti National Centre for Epidemiology H-1097 Budapest Gyáli út 2-6 Hungary

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T. Mag

T. Mag National Centre for Epidemiology H-1097 Budapest Gyáli út 2-6 Hungary

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P. Kovács

P. Kovács Szent István University Faculty of Veterinary Science H-1400 Budapest P.O. Box 2 Hungary

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G. Kovács

G. Kovács Hungarian Defence Forces “Dr. György Radó” Military Medical Centre H-1097 Budapest Gyáli út 17-19 Hungary

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J. Szita

J. Szita National Institute of Traumatology and Emergency Medicine H-1081 Budapest Fiumei út 17 Hungary

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P. Tóth

P. Tóth Szent István University Faculty of Veterinary Science H-1400 Budapest P.O. Box 2 Hungary

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I. Szatmári

I. Szatmári Szent István University Faculty of Veterinary Science H-1400 Budapest P.O. Box 2 Hungary

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S. Bernáth

S. Bernáth Directorate of Veterinary Medicinal Products Central Agricultural Office H-1475 Budapest P.O. Box 318 Hungary

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Pages:: 32–37

Online Publication Date:: 23 Dec 2010

Publication Date:: 01 Mar 2011

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/aalim.2010.0013

Keywords:: verocytotoxin; isolation; pasteurized milk samples; low pasteurization temperature

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The authors investigated the possibility of the presence of VTEC strains in improperly pasteurized milk samples. A total of 64 Escherichia coli strains were isolated from 135 pasteurized milk samples originating from the same producer. The examined isolates contained 29 haemolysin-, 9 colicin- and 5 aerobactin-producing strains, but the investigations concerning heat-resistant and heat-sensitive toxins gave negative results.Six O128-type E. coli strains exerted a cytotoxic effect on the VERO cell line; 5 of them contained H12 antigen, while one could not be typed. Four of the 6 verocytotoxin-producing strains belonged in phage group 20, one in phage group (2)3(7), and one in phage group 4; four strains were of B3, one of A1, and one of A1(A2) phage type.Because of a technical failure the milk was pasteurized at 69 °C for 15 s, which is 2 °C less than required. The results underline the importance of the appropriate pasteurization temperature, as otherwise the milk may contain verocytotoxin-producing E. coli, which is a potentially great hazard for public health.

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Acta Alimentaria

Print ISSN:: 0139-3006

Online ISSN:: 1588-2535

Editorial Board

László Abrankó, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
Tamás Antal, University of Nyíregyháza, Nyíregyháza, Hungary
Diána Bánáti, University of Szeged, Szeged, Hungary
József Baranyi, Institute of Food Research, Norwich, UK
Ildikó Bata-Vidács, Eszterházy Károly Catholic University, Eger, Hungary
Ferenc Békés, FBFD PTY LTD, Sydney, NSW Australia
György Biró, Budapest, Hungary
Anna Blázovics, Semmelweis University, Budapest, Hungary
Francesco Capozzi, University of Bologna, Bologna, Italy
Marina Carcea, Research Centre for Food and Nutrition, Council for Agricultural Research and Economics Rome, Italy
Zsuzsanna Cserhalmi, Budapest, Hungary
Marco Dalla Rosa, University of Bologna, Bologna, Italy
István Dalmadi, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
Katarina Demnerova, University of Chemistry and Technology, Prague, Czech Republic
Mária Dobozi King, Texas A&M University, Texas, USA
Muying Du, Southwest University in Chongqing, Chongqing, China
Sedef Nehir El, Ege University, Izmir, Turkey
Søren Balling Engelsen, University of Copenhagen, Copenhagen, Denmark
Éva Gelencsér, Budapest, Hungary
Vicente Manuel Gómez-López, Universidad Católica San Antonio de Murcia, Murcia, Spain
Jovica Hardi, University of Osijek, Osijek, Croatia
Hongju He, Henan Institute of Science and Technology, Xinxiang, China
Károly Héberger, Research Centre for Natural Sciences, ELKH, Budapest, Hungary
Nebojsa Ilić, University of Novi Sad, Novi Sad, Serbia
Dietrich Knorr, Technische Universität Berlin, Berlin, Germany
Hamit Köksel, Hacettepe University, Ankara, Turkey
Katia Liburdi, Tuscia University, Viterbo, Italy
Meinolf Lindhauer, Max Rubner Institute, Detmold, Germany
Min-Tze Liong, Universiti Sains Malaysia, Penang, Malaysia
Marena Manley, Stellenbosch University, Stellenbosch, South Africa
Miklós Mézes, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary
Áron Németh, Budapest University of Technology and Economics, Budapest, Hungary
Perry Ng, Michigan State University, Michigan, USA
Quang Duc Nguyen, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary
Laura Nyström, ETH Zürich, Switzerland
Lola Perez, University of Cordoba, Cordoba, Spain
Vieno Piironen, University of Helsinki, Finland
Alessandra Pino, University of Catania, Catania, Italy
Mojmir Rychtera, University of Chemistry and Technology, Prague, Czech Republic
Katharina Scherf, Technical University, Munich, Germany
Regine Schönlechner, University of Natural Resources and Life Sciences, Vienna, Austria
Arun Kumar Sharma, Department of Atomic Energy, Delhi, India
András Szarka, Budapest University of Technology and Economics, Budapest, Hungary
Mária Szeitzné Szabó, Budapest, Hungary
Sándor Tömösközi, Budapest University of Technology and Economics, Budapest, Hungary
László Varga, Széchenyi István University, Mosonmagyaróvár, Hungary
Rimantas Venskutonis, Kaunas University of Technology, Kaunas, Lithuania
Barbara Wróblewska, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olsztyn, Poland

Acta Alimentaria
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Acta Alimentaria
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Acta Alimentaria
Language	English
Size	B5
Year of Foundation	1972
Volumes per Year	1
Issues per Year	4
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
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ISSN	0139-3006 (Print)
ISSN	1588-2535 (Online)