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  • 1 A.L. Instruments H-2112 Veresegyház Mester u. 9 Hungary
  • 2 Szent István University Faculty of Veterinary Science H-1400 Budapest P.O. Box 2 Hungary
  • 3 National Centre for Epidemiology H-1097 Budapest Gyáli út 2-6 Hungary
  • 4 Hungarian Defence Forces “Dr. György Radó” Military Medical Centre H-1097 Budapest Gyáli út 17-19 Hungary
  • 5 National Institute of Traumatology and Emergency Medicine H-1081 Budapest Fiumei út 17 Hungary
  • 6 Directorate of Veterinary Medicinal Products Central Agricultural Office H-1475 Budapest P.O. Box 318 Hungary
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The authors investigated the possibility of the presence of VTEC strains in improperly pasteurized milk samples. A total of 64 Escherichia coli strains were isolated from 135 pasteurized milk samples originating from the same producer. The examined isolates contained 29 haemolysin-, 9 colicin- and 5 aerobactin-producing strains, but the investigations concerning heat-resistant and heat-sensitive toxins gave negative results.Six O128-type E. coli strains exerted a cytotoxic effect on the VERO cell line; 5 of them contained H12 antigen, while one could not be typed. Four of the 6 verocytotoxin-producing strains belonged in phage group 20, one in phage group (2)3(7), and one in phage group 4; four strains were of B3, one of A1, and one of A1(A2) phage type.Because of a technical failure the milk was pasteurized at 69 °C for 15 s, which is 2 °C less than required. The results underline the importance of the appropriate pasteurization temperature, as otherwise the milk may contain verocytotoxin-producing E. coli, which is a potentially great hazard for public health.

  • Aidar-Ugrinovich, L., Blanco, J., Blanco, M., Blanco, J.E., Leomil, L., Dahbi, G., Mora, A., Onuma, D.L., Silveira, W.D. & Pestana De Castro, A.F. (2007): Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in Sao Paulo, Brazil. Int. J. Fd Microbiol., 115, 297–306.

    Pestana De Castro A.F. , 'Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in Sao Paulo, Brazil ' (2007 ) 115 Int. J. Fd Microbiol. : 297 -306.

    • Search Google Scholar
  • Blanco, M., Blanco, J.E., Mora, A., Rey, J., Alonso, J.M., Hermoso, M., Hermoso, J., Alonso, M.P., Dahbi, G., González, E.A., Bernárdez, M.I. & Blanco, J. (2003): Serotypes, virulence genes, and intimin types of shiga toxin (verotoxin)-producing Escherichia coli isolates from healthy sheep in Spain. J. clin. Microbiol., 41, 1351–1356.

    Blanco J. , 'Serotypes, virulence genes, and intimin types of shiga toxin (verotoxin)-producing Escherichia coli isolates from healthy sheep in Spain ' (2003 ) 41 J. clin. Microbiol. : 1351 -1356.

    • Search Google Scholar
  • Brooks, H.J.L., Bettelheim, K.A., Todd, B. & Holdaway, M.D. (1997): Non-O157 vero cytotoxin producing Escherichia coli: aetiological agents of diarrhoea in children in Dunedin, New Zealand. Comparative Immunol., Microbiol. inf. Dis., 20, 163–170.

    Holdaway M.D. , 'Non-O157 vero cytotoxin producing Escherichia coli: aetiological agents of diarrhoea in children in Dunedin, New Zealand ' (1997 ) 20 Comparative Immunol., Microbiol. inf. Dis. : 163 -170.

    • Search Google Scholar
  • Caprioli, A., Morabito, S., Scheutz, F., Chart, H., Oswald, E., Brigotti, M., Monnens, L., Aspan, A., La Ragione, R., Low, C. & Newell, D. (2006): Pathogenesis of verotoxin/shiga toxin producing Escherichia coli infection. Emerg. inf. Dis. (serial on the Internet). Conference summary, Rome, Italy. December 5–7, 2005) Vol. 12, No. 8. Available from http://www.cdc.gov/ncidod/EID/vol12no08/06-0170.htm

  • Denny, J., Hernandez-Pezzi, G., Threfall, J., Westrell, T. & Fischer, I. (2008): A quarterly update on food- and waterborne diseases in Europe — summary of data for the third quarter of 2007. Euro. Surveill. 13(11). Available online: http://www.eurosurveillance.org/edition/v13n11/080313_4.asp

  • European Commission (2003): Opinion of the Scientific Committee on veterinary measures relating to public health on verotoxigenic E. coli (VTEC) in foodstuffs. (Adopted on 21–22 January 2003). http://ec.europa.eu/food/fs/sc/scv/out58_en.pdf

  • Herpay, M., Czirók, É., Nyomárkai, I., Mitro, E. & Pászti, J. (1994): Verocytotoxin-producing Escherichia coli isolates in Hungary. Alpe-Adria Microbiol. J., 3, 205–207.

    Pászti J. , 'Verocytotoxin-producing Escherichia coli isolates in Hungary ' (1994 ) 3 Alpe-Adria Microbiol. J. : 205 -207.

    • Search Google Scholar
  • Hungarian Patent (1987): Eljárás és szintetikus, szelektív táptalaj kóliform baktériumok kimutatására. (Procedure and synthetic medium for selective determination of coliform bacteria.) No. C112 N 1620, Hungarian Patent Office.

  • ISO/DIS (1979): Meat and meat products — Detection and enumeration of presumptive coliform bacteria and presumptive Escherichia coli. No 3811, International Organisation For Standardisation (1979).

  • Konowalchuk, J., Speirs, J.I. & Stavric, S. (1977): Vero response to a cytotoxin of Escherichia coli. Infect. Immunity, 18, 775–779.

    Stavric S. , 'Vero response to a cytotoxin of Escherichia coli ' (1977 ) 18 Infect. Immunity : 775 -779.

    • Search Google Scholar
  • Lynn, R.M., O’Brien, S.J., Taylor, C.M., Adak, G.K., Chart, H., Cheasty, T., Coia, J.E., Gillespie, I.A., Locking, M.E., Reilly, W.J., Smith, H.R., Waters, A. & Willshaw, G.A. (2005): Childhood hemolytic uremic syndrome in United Kingdom and Ireland. Emerg. inf. Dis., 11, 590–596.

    Willshaw G.A. , 'Childhood hemolytic uremic syndrome in United Kingdom and Ireland ' (2005 ) 11 Emerg. inf. Dis. : 590 -596.

    • Search Google Scholar
  • Mora, A., León, S.L., Blanco, M., Blanco, J.E., López, C., Dahbi, G., Echeita, A., Gonzalez, E.A. & Blanco, J. (2007): Phage types, virulence genes and PFGE profiles of Shiga toxin-producing Escherichia coli O157:H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru). Int. J. Fd Microbiol., 114, 204–210.

    Blanco J. , 'Phage types, virulence genes and PFGE profiles of Shiga toxin-producing Escherichia coli O157:H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru) ' (2007 ) 114 Int. J. Fd Microbiol. : 204 -210.

    • Search Google Scholar
  • Nguyen, R.N., Taylor, L.S., Tauschek, M. & Robins-Browne, R.M. (2006): Atypical enteropathogenic Escherichia coli infection and prolonged diarrhea in children. Emerg. inf. Dis., 12, 597–603.

    Robins-Browne R.M. , 'Atypical enteropathogenic Escherichia coli infection and prolonged diarrhea in children ' (2006 ) 12 Emerg. inf. Dis. : 597 -603.

    • Search Google Scholar
  • Quinto, E.J. & Cepeda, A. (1997): Incidence of toxigenic Escherichia coli in soft cheese made with raw or pasteurized milk. Lett. appl. Microbiol., 24, 291–295.

    Cepeda A. , 'Incidence of toxigenic Escherichia coli in soft cheese made with raw or pasteurized milk ' (1997 ) 24 Lett. appl. Microbiol. : 291 -295.

    • Search Google Scholar
  • [scRILEY, L.W., REMIS, R.S., HELGERSON, S.D., MCGEE, H.B., WELLS, J.G., DAVIS, B.R., HERBERT, R.J., OLCOTT, E.S., JOHNSON, L.M., HARGRETT, N.T., BLAKE, P.A. & COHEN, M.L. (1983): Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med., 308, 681–685.

    Cohen M.L. , 'Hemorrhagic colitis associated with a rare Escherichia coli serotype ' (1983 ) 308 N. Engl. J. Med. : 681 -685.

    • Search Google Scholar
  • Sakuma, M., Urashima, M. & Okabe, N. (2006): Verotoxin-producing Escherichia coli, Japan, 1999–2004. Emerg. inf. Dis., 12, 323–325.

    Okabe N. , 'Verotoxin-producing Escherichia coli, Japan, 1999–2004 ' (2006 ) 12 Emerg. inf. Dis. : 323 -325.

    • Search Google Scholar
  • Szita, G., Schmidt, E., Bernáth, S., Faludi, G., Fábián, A., Tabajdi, V. & Kiss, R. (2003): A synthetic culture medium evaluated for detection of coliform bacteria in tomato paprika. Fd Microbiol., 20, 483–486.

    Kiss R. , 'A synthetic culture medium evaluated for detection of coliform bacteria in tomato paprika ' (2003 ) 20 Fd Microbiol. : 483 -486.

    • Search Google Scholar
  • Timm, C.D.K., Irino, T.A.T., Gomes, M.M., Vieira, B.E.C., Guth, T.M.I. & Vaz, C. (2007): Virulence markers and serotyes of shiga toxin-producing Escherichia coli, isolated from cattle in Rio Grande do Sul, Brazil. Lett. appl. Microbiol., 44, 419–425.

    Vaz C. , 'Virulence markers and serotyes of shiga toxin-producing Escherichia coli, isolated from cattle in Rio Grande do Sul, Brazil ' (2007 ) 44 Lett. appl. Microbiol. : 419 -425.

    • Search Google Scholar
  • Vali, L., Hamouda, A., Pearce, M.C., Knight, H.I., Evans, J. & Amyes, S.G.B. (2007): Detection of genetic diversity by pulsed-field gel electrophoresis among Escherichia coli O157 isolated from bovine faecal samples by immunomagnetic separation technique. Lett. appl. Microbiol., 44, 19–23.

    Amyes S.G.B. , 'Detection of genetic diversity by pulsed-field gel electrophoresis among Escherichia coli O157 isolated from bovine faecal samples by immunomagnetic separation technique ' (2007 ) 44 Lett. appl. Microbiol. : 19 -23.

    • Search Google Scholar
  • Varela-Hernández, J.J., Cabrera-Diaz, E., Cardona-López, M.A., Ibarra-Velázquez, L.M., Rangel-Villalobos, H., Castillo, A., Torres-Vitela, M.R. & Ramírez-Alvarez, A. (2007): Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 from beef carcasses at a slaughter plant in Mexico. Int. J. Fd Microbiol., 113, 237–241.

    Ramírez-Alvarez A. , 'Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 from beef carcasses at a slaughter plant in Mexico ' (2007 ) 113 Int. J. Fd Microbiol. : 237 -241.

    • Search Google Scholar
  • Vaz, T.M.I., Irino, K., Kato, M.A.M.F., Dias, A.M.G., Gomes, T.A.T. & Medeiros, M.I.C. (2004): Virulence properties and characteristics of shiga toxin-producing Escherichia coli in São Paulo, Brazil, from 1976 through 1999. J. clin. Microbiol., 42, 903–905.

    Medeiros M.I.C. , 'Virulence properties and characteristics of shiga toxin-producing Escherichia coli in São Paulo, Brazil, from 1976 through 1999 ' (2004 ) 42 J. clin. Microbiol. : 903 -905.

    • Search Google Scholar
  • Wachsmuth, I.K., Sparling, P.H., Barret, T.J. & Potter, M.E. (1997): Enterohemorrhagic Escherichia coli in the United States. FEMS, Immunol. med. Microbiol., 18, 2343–2349.

    Potter M.E. , 'Enterohemorrhagic Escherichia coli in the United States ' (1997 ) 18 FEMS, Immunol. med. Microbiol. : 2343 -2349.

    • Search Google Scholar

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