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  • 1 Universidad Autónoma de Nuevo León. Apdo Postal 124-F Departmento de Microbiología e Inmunología, Facultad de Ciencias Biológicas San Nicolás N.L. 66451 México
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The aim of this study was to investigate the effect of some food additives used in foods on cold tolerance of Clostridium perfringens at pH close to neutral.Maximal concentrations recommended for foods of sodium benzoate, potassium sorbate, sodium nitrite, monosodium glutamate, or mixtures of those were added to cultures and their effects on C. perfringens tolerance to 10 °C were evaluated. The effect of a previous shock at 28 °C was also determined. Growth of C. perfringens was not inhibited by the substances examined. Sodium nitrite, applied at maximal permitted concentrations, increased C. perfringens survival at 10 °C. Mixtures of GRAS compounds had either no clear effect, or increased tolerance to 10 °C. A pre-shock (28 °C) of the cultures treated with sodium benzoate, sodium nitrite or monosodium glutamate increased survival and stimulated growth of the cultures treated at 10 °C.We conclude that the addition of these compounds including sodium benzoate, potassium sorbate, sodium nitrite and monosodium glutamate to cultures of C. perfringens can influence their cold tolerance. In some cases, the substances that would normally eliminate microorganisms at lower pH, can increase tolerance of this bacterium, permitting survival at low temperatures.

  • Banerjee, M. & Sarkar, P.K. (2004): Antibiotic resistance and susceptibility to some food preservative measures of spoilage and pathogenic microorganisms from spices. Fd Microbiol., 21, 335–342.

    Sarkar P.K. , 'Antibiotic resistance and susceptibility to some food preservative measures of spoilage and pathogenic microorganisms from spices ' (2004 ) 21 Fd Microbiol. : 335 -342.

    • Search Google Scholar
  • Beales, N. (2004): Adaptation of microorganisms to cold temperatures, weak acid preservatives, low pH, and osmotic stress: A review. Comp. Rev. Fd Sci. Fd Safety, 3, 1–20.

    Beales N. , 'Adaptation of microorganisms to cold temperatures, weak acid preservatives, low pH, and osmotic stress: A review ' (2004 ) 3 Comp. Rev. Fd Sci. Fd Safety : 1 -20.

    • Search Google Scholar
  • Buchanan, R.L., Edlosn-Mammel, S.G., Boyd, G. & Marmer, B.S. (2004): Influence of acidulant identity on the effects of pH and acid resistance on the radiation resistance of Escherichia coli O157:H7. Fd Microbiol., 21, 51–57.

    Marmer B.S. , 'Influence of acidulant identity on the effects of pH and acid resistance on the radiation resistance of Escherichia coli O157:H7 ' (2004 ) 21 Fd Microbiol. : 51 -57.

    • Search Google Scholar
  • Chipley, J.R. (2005): Sodium benzoate and benzoic acid. -in: Davidson, P.M., Sofos, J.N. & Branen, A.L. (Eds) Antimicrobials in foods. CRC Press, Boca Raton, pp. 11–48.

    Chipley J.R. , '', in Antimicrobials in foods , (2005 ) -.

  • Collie, R.E. & McClane, B.A. (1998): Evidence that the enterotoxin gene can be episomal in Clostridium perfringens isolates associated with non-food-borne human gastrointestinal disease. J. clin. Microbiol., 36, 30–36.

    McClane B.A. , 'Evidence that the enterotoxin gene can be episomal in Clostridium perfringens isolates associated with non-food-borne human gastrointestinal disease ' (1998 ) 36 J. clin. Microbiol. : 30 -36.

    • Search Google Scholar
  • Davidson, P.M. & Harrison, M.A. (2002): Resistance and adaptation to food antimicrobials, sanitizers, and other process controls. Fd Technol., 56, 69–78.

    Harrison M.A. , 'Resistance and adaptation to food antimicrobials, sanitizers, and other process controls ' (2002 ) 56 Fd Technol. : 69 -78.

    • Search Google Scholar
  • De Joung, A.E., Rombouts, F.M. & Beumer, R.R. (2004): Behavior of Clostridium perfringens at low temperatures. Int. J. Fd Microbiol., 97, 71–80.

    Beumer R.R. , 'Behavior of Clostridium perfringens at low temperatures ' (2004 ) 97 Int. J. Fd Microbiol. : 71 -80.

    • Search Google Scholar
  • Drummond, L.J., Smith, D.G.E. & Poxton, I.R. (2003): Effects of sub-MIC concentrations of antibiotics on growth of and toxin production by Clostridium difficile. J. med. Microbiol., 52, 1033–1038.

    Poxton I.R. , 'Effects of sub-MIC concentrations of antibiotics on growth of and toxin production by Clostridium difficile ' (2003 ) 52 J. med. Microbiol. : 1033 -1038.

    • Search Google Scholar
  • Garcia, S., Limon, J.C. & Heredia, N.L. (2001): Cross protection by heat and cold shocks to lethal temperatures in Clostridium perfringens. Braz. J. Microbiol., 32, 110–112.

    Heredia N.L. , 'Cross protection by heat and cold shocks to lethal temperatures in Clostridium perfringens ' (2001 ) 32 Braz. J. Microbiol. : 110 -112.

    • Search Google Scholar
  • Garcia-Alvarado, J.S., Rodriguez, M.A. & Labbee, R.G. (1992): Influence of elevated temperatures on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type A at 37 and 43 °C. Appl. environ. Microbiol., 58, 326–330.

    Labbee R.G. , 'Influence of elevated temperatures on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type A at 37 and 43 °C ' (1992 ) 58 Appl. environ. Microbiol. : 326 -330.

    • Search Google Scholar
  • Heredia, N.L. & Labbee, R.G. (2001): Clostridium perfringens. -in: Labbe, R.G. & Garcia, S. (Eds) Guide to foodborne pathogens. John Wiley & Sons, New York, pp. 133–142.

    Labbee R.G. , '', in Guide to foodborne pathogens , (2001 ) -.

  • Heredia, N.L., Garcia, G.A., Luevanos, R., Labbee, R.G. & Garcia-Alvarado, J.S. (1997): Elevation of the heat resistance of vegetative cells and spores of Clostridium perfringens type A by sublethal heat shock. J. Fd Prot., 60, 998–1000.

    Garcia-Alvarado J.S. , 'Elevation of the heat resistance of vegetative cells and spores of Clostridium perfringens type A by sublethal heat shock ' (1997 ) 60 J. Fd Prot. : 998 -1000.

    • Search Google Scholar
  • Jones, P.G. & Inouye, M. (1994): The cold-shock response — a hot topic. Molec. Microbiol., 11, 811–818.

    Inouye M. , 'The cold-shock response — a hot topic ' (1994 ) 11 Molec. Microbiol. : 811 -818.

    • Search Google Scholar
  • Juneja, V.K. & Marmer, B.S. (1998): Thermal inactivation of Clostridium perfringens vegetative cells in ground beef and turkey as affected by sodium pyrophosphate. Fd Microbiol., 15, 281–287.

    Marmer B.S. , 'Thermal inactivation of Clostridium perfringens vegetative cells in ground beef and turkey as affected by sodium pyrophosphate ' (1998 ) 15 Fd Microbiol. : 281 -287.

    • Search Google Scholar
  • Langsrud, S., Baardsen, B. & Sundheim, G. (2000): Potentiation of the lethal effect of peroxygen on Bacillus cereus spores by alkali and enzyme wash. Int. J. Fd Microbiol., 56, 81–86.

    Sundheim G. , 'Potentiation of the lethal effect of peroxygen on Bacillus cereus spores by alkali and enzyme wash ' (2000 ) 56 Int. J. Fd Microbiol. : 81 -86.

    • Search Google Scholar
  • Li, J. & McClane, B.A. (2006): Comparative effects of osmotic, sodium nitrite-induced and pH induced stress on growth and survival of Clostridium perfringens type A isolates carrying chromosomal or plasmid-borne enterotoxin genes. Appl. environ. Microbiol., 72, 7620–7625.

    McClane B.A. , 'Comparative effects of osmotic, sodium nitrite-induced and pH induced stress on growth and survival of Clostridium perfringens type A isolates carrying chromosomal or plasmid-borne enterotoxin genes ' (2006 ) 72 Appl. environ. Microbiol. : 7620 -7625.

    • Search Google Scholar
  • Lopez, M., Martinez, S., Gonzalez, J., Martin, R. & Bernardo, A. (1998): Sensitization of thermally injured spores of Bacillus stearothermophilus to sodium benzoate and potassium sorbate. Lett. appl. Microbiol., 27, 331–335.

    Bernardo A. , 'Sensitization of thermally injured spores of Bacillus stearothermophilus to sodium benzoate and potassium sorbate ' (1998 ) 27 Lett. appl. Microbiol. : 331 -335.

    • Search Google Scholar
  • Sagoo, S.K., Board, R. & Roller, S. (2002): Chitosan potentiates the antimicrobial action of sodium benzoate on spoilage yeasts. Lett. appl. Microbiol., 34, 168–172.

    Roller S. , 'Chitosan potentiates the antimicrobial action of sodium benzoate on spoilage yeasts ' (2002 ) 34 Lett. appl. Microbiol. : 168 -172.

    • Search Google Scholar
  • USFDA (2003): Food Additives, sec 170.30 [Eligibility for classification as generally recognized as safe (GRAS)]. United States Food and Drug Administration. Available at: http://www.foodsafety.gov/~lrd/fcf170.html . Accessed August 14, 2009

  • Villarreal, L., Heredia, N.L. & Garcia, S. (2002): Cold tolerance and changes in rates of synthesis of individual proteins of Clostridium perfringens as a result of cold pre-treatment. Acta Alimentaria, 31, 179–184.

    Garcia S. , 'Cold tolerance and changes in rates of synthesis of individual proteins of Clostridium perfringens as a result of cold pre-treatment ' (2002 ) 31 Acta Alimentaria : 179 -184.

    • Search Google Scholar
  • Zar, J.H. (1996): Biostatistical analysis. Prentice-Hall Inc., Upper Saddle River, New Jersey, pp. 124–130.

    Zar J.H. , '', in Biostatistical analysis , (1996 ) -.

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