Acta Alimentaria
Volume/Issue:
Volume 40: Issue Issue Supplement-1
Molecular analysis of poultry meat spoiling microbiota and heterogeneity of their proteolytic and lipolytic enzyme activities
Authors: Á. Belák 1 , M. Kovács 1 , Zs. Hermann 1 , Á. Holczman 1 , D. Márta 1 , S. Stojakovič 2 , N. Bajcsi 1 , and A. Maráz 1
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  • 1 Corvinus University of Budapest Department of Microbiology and Biotechnology, Faculty of Food Science H-1118 Budapest Somlói út 14-16 Hungary
  • 2 University of Zagreb Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology Pierottijeva 6 10000 Zagreb Croatia
DOI:
https://doi.org/10.1556/aalim.40.2011.suppl.2
Page Count:
3–22
Publication Date:
01 Jan 2011
Online Publication Date:
07 Sep 2011
Article Category:
Research Article
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Psychrotrophic Pseudomonas species P. fluorescens, P. fragi and P. lundensis were found as predominant bacteria of chicken meat stored at chill temperature, which showed high level of molecular diversity, while isolates of the psychrotrophic yeasts Candida zeylanoides, Metschnikowia pulcherrima, Rhodotorula glutinis and Rhodotorula mucilaginosa formed clusters of high level similarity within the different species as revealed by RAPD-PCR analysis.Combination of multiplex PCR and sequencing of the rpoB gene resulted correct identification of the Pseudomas isolates, while the routine diagnostic tests led to improper identification in case of half of the isolates, which indicated the extended biochemical and physiological heterogeneity of the food-borne pseudomonads. Majority of P. fluorescens and P. lundensis isolates were strong protease and lipase producers, while P. fragi strains were week or negative from this respect. Proteolytic and lipolytic activities of the isolated yeast strains were species specific and protease production was less frequent than lipolytic activities.

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Cover Acta Alimentaria
Acta Alimentaria
Print ISSN:
0139-3006
Online ISSN:
1588-2535
Keywords:
poultry meat; spoilage; Pseudomonas ; yeasts; molecular characterisation; protease; lipase

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