View More View Less
  • 1 Corvinus University of Budapest Department of Microbiology and Biotechnology, Faculty of Food Science H-1118 Budapest Somlói út 14-16 Hungary
  • 2 University of Zagreb Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology Pierottijeva 6 10000 Zagreb Croatia
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

Psychrotrophic Pseudomonas species P. fluorescens, P. fragi and P. lundensis were found as predominant bacteria of chicken meat stored at chill temperature, which showed high level of molecular diversity, while isolates of the psychrotrophic yeasts Candida zeylanoides, Metschnikowia pulcherrima, Rhodotorula glutinis and Rhodotorula mucilaginosa formed clusters of high level similarity within the different species as revealed by RAPD-PCR analysis.Combination of multiplex PCR and sequencing of the rpoB gene resulted correct identification of the Pseudomas isolates, while the routine diagnostic tests led to improper identification in case of half of the isolates, which indicated the extended biochemical and physiological heterogeneity of the food-borne pseudomonads. Majority of P. fluorescens and P. lundensis isolates were strong protease and lipase producers, while P. fragi strains were week or negative from this respect. Proteolytic and lipolytic activities of the isolated yeast strains were species specific and protease production was less frequent than lipolytic activities.

  • Abranches, J., Morais, P.B., Rosa, C.A., Mendonca-hagler, L.C. & Hagler, H.N. (1997): The incidence of killer activity and extracellular proteases in tropical yeast communities. Can. J. Microbiol., 43, 328–336.

    Hagler H.N. , 'The incidence of killer activity and extracellular proteases in tropical yeast communities ' (1997 ) 43 Can. J. Microbiol. : 328 -336.

    • Search Google Scholar
  • Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. (2000): Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int. J. syst. evol. Microbiol., 50, 1563–1589.

    Oyaizu H. , 'Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence ' (2000 ) 50 Int. J. syst. evol. Microbiol. : 1563 -1589.

    • Search Google Scholar
  • Arpigni, J.L. & Jeager, K-L. (1999): Bacterial lipolytic enzymes: classification and properties. Biochem. J., 343, 177–183.

    Jeager K.-L. , 'Bacterial lipolytic enzymes: classification and properties ' (1999 ) 343 Biochem. J. : 177 -183.

    • Search Google Scholar
  • Atlas, R.M. (1995): Handbook of microbiological media for the examination of food. CRC Press, Florida, pp. 1–273

    Atlas R.M. , '', in Handbook of microbiological media for the examination of food. , (1995 ) -.

    • Search Google Scholar
  • Baleiras Couto, M.M., Vogels J.T.W.E., Hofstra, H., Huis In’t Veld, J.H.J. & van Der Vossen, J.M.B.M. (1995): Random amplified polymorphic DNA and restriction enzyme analysis of PCR amplified rDNA in taxonomy: two identification techniques for food-borne yeasts. J. appl. Bacteriol., 79, 525–535.

    Vossen J.M.B.M. , 'Random amplified polymorphic DNA and restriction enzyme analysis of PCR amplified rDNA in taxonomy: two identification techniques for food-borne yeasts ' (1995 ) 79 J. appl. Bacteriol. : 525 -535.

    • Search Google Scholar
  • Barnes, E.M., Impey, C.S., Geeson, J.D. & Buhagiar, R.W.M. (1978): The effect of storage temperature on the shelf-life of eviscerated air chilled turkeys. Br. Poult. Sci., 19, 77–84.

    Buhagiar R.W.M. , 'The effect of storage temperature on the shelf-life of eviscerated air chilled turkeys ' (1978 ) 19 Br. Poult. Sci. : 77 -84.

    • Search Google Scholar
  • Boekhout, T. & Robert, V. (2003): Yeasts in food. Beneficial and detrimental aspects. B. Behr’s Verlag GmbH & Co., Hamburg, pp. 1–488

    Robert V. , '', in Yeasts in food. Beneficial and detrimental aspects. , (2003 ) -.

  • Corzo, G. & Revah, S. (1999): Production and characteristics of the lipase from Yarrowia lipolytica 681. Bioresource Technology, 70, 173–180.

    Revah S. , 'Production and characteristics of the lipase from Yarrowia lipolytica 681 ' (1999 ) 70 Bioresource Technology : 173 -180.

    • Search Google Scholar
  • Daud, H.B. & Mcmeekin, T.A. & Thomas, C.J. (1979): Spoilage association of chicken skin. Appl. environ. Microbiol., 37, 399–401.

    Thomas C.J. , 'Spoilage association of chicken skin ' (1979 ) 37 Appl. environ. Microbiol. : 399 -401.

    • Search Google Scholar
  • Deák, T. (2008): Handbook of food spoilage yeasts. CRC Press, Boca Raton, FL, pp. 1–325.

    Deák T. , '', in Handbook of food spoilage yeasts. , (2008 ) -.

  • Dogan, B. & Boor, K.J. (2003): Genetic diversity and spoilage potentials among Pseudomonas spp. isolated from fluid milk products and dairy processing plants. Appl. environ. Microbiol., 69, 130–138.

    Boor K.J. , 'Genetic diversity and spoilage potentials among Pseudomonas spp. isolated from fluid milk products and dairy processing plants ' (2003 ) 69 Appl. environ. Microbiol. : 130 -138.

    • Search Google Scholar
  • Ercolini, D., Russo, F., Blaiotta, G., Pepe, O., Mauriello, G. & Villani, F. (2007): Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene. Appl. environ. Microbiol., 73, 2354–2359.

    Villani F. , 'Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene ' (2007 ) 73 Appl. environ. Microbiol. : 2354 -2359.

    • Search Google Scholar
  • Ercolini, D., Russo, F., Nasi, A., Ferranti, P. & Villani, F. (2009): Mesophilic and psychrotrophic bacteria from meat and their spoiling potential in vitro and in beef. Appl. environ. Microbiol., 75, 1990–2001.

    Villani F. , 'Mesophilic and psychrotrophic bacteria from meat and their spoiling potential in vitro and in beef ' (2009 ) 75 Appl. environ. Microbiol. : 1990 -2001.

    • Search Google Scholar
  • Gallo, L., Schmitt, R.E. & Schmidt-Lorenz, W. (1998): Microbial spoilage of refrigerated fresh broilers. I. Bacterial flora and growth during storage. Lebensm. Wiss. Technol., 21, 216–223.

    Schmidt-Lorenz W. , 'Microbial spoilage of refrigerated fresh broilers. I. Bacterial flora and growth during storage ' (1998 ) 21 Lebensm. Wiss. Technol. : 216 -223.

    • Search Google Scholar
  • Geornaras, I., DE Jesus, A., VAN Zyl, E. & VON Holy, A. (1995): Microbiological survey of a South African poultry processing plant. J. basic Microbiol., 35, 73–82.

    Holy A. , 'Microbiological survey of a South African poultry processing plant ' (1995 ) 35 J. basic Microbiol. : 73 -82.

    • Search Google Scholar
  • Gilmour, A. & Rowe, W.T. (1990): Microorganisms associated with milk. -in: Robinson, N.K. (Ed.) Dairy microbiology Vol. 1, Elsevier Applied Science, London, pp. 37–75.

    Rowe W.T. , '', in Dairy microbiology Vol. 1 , (1990 ) -.

  • Gupta, R., Gupta, N. & Rathi, P. (2004): Bacterial lipases: an overview of production, purification and biochemical properties. Appl. Microbiol. Biotechnol., 64, 763–781.

    Rathi P. , 'Bacterial lipases: an overview of production, purification and biochemical properties ' (2004 ) 64 Appl. Microbiol. Biotechnol. : 763 -781.

    • Search Google Scholar
  • Hoffman, S. & Winston, F. (1987): A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57, 267–272.

    Winston F. , 'A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. ' (1987 ) 57 Gene : 267 -272.

    • Search Google Scholar
  • Horváth, K., Andrássy, É., Korbász, M. & Farkas, J. (2007a): Using automatic conductometry for monitoring spoilage bacteria on chilled pork cutlets. Acta Alimentaria, 36, 283–291.

    Farkas J. , 'Using automatic conductometry for monitoring spoilage bacteria on chilled pork cutlets ' (2007 ) 36 Acta Alimentaria : 283 -291.

    • Search Google Scholar
  • Horváth, K., Seregély, Zs., Dalmadi, I., Andrássy, É. & Farkas, J. (2007b): Estimation of bacteriological spoilage of pork cutlets by electronic nose. Acta Microbiol. Immunol. Hung., 54, 179–194.

    Farkas J. , 'Estimation of bacteriological spoilage of pork cutlets by electronic nose ' (2007 ) 54 Acta Microbiol. Immunol. Hung. : 179 -194.

    • Search Google Scholar
  • Hsieh, D.Y. & Jay, J.M. (1984): Characterization and identification of yeasts from fresh and spoiled ground beef. Int. J. Fd Microbiol., 1, 141–147.

    Jay J.M. , 'Characterization and identification of yeasts from fresh and spoiled ground beef ' (1984 ) 1 Int. J. Fd Microbiol. : 141 -147.

    • Search Google Scholar
  • Huis In’t Veld, J.H.J. (1996): Microbial and biochemical spoilage of foods: an overview. Int. J. Fd Microbiol., 33, 1–18.

    Huis In’t Veld J.H.J. , 'Microbial and biochemical spoilage of foods: an overview ' (1996 ) 33 Int. J. Fd Microbiol. : 1 -18.

    • Search Google Scholar
  • Ismail, S.A.S., Deak, T., Abd-Rahman, H.A., Zassien, M.A.M. & Beuchat, L.R. (2000): Presence and changes in populations of yeasts associated with raw and processed poultry products stored at refrigeration temperature. Int. J. Fd Microbiol, 62, 113–121.

    Beuchat L.R. , 'Presence and changes in populations of yeasts associated with raw and processed poultry products stored at refrigeration temperature ' (2000 ) 62 Int. J. Fd Microbiol : 113 -121.

    • Search Google Scholar
  • Jasson, V., Jacxsens, L., Luning, P., Rajkovic, A. & Uyttendaele, M. (2010): Alternative microbial methods: An overview and selection criteria. Fd Microbiol., 27, 710–730.

    Uyttendaele M. , 'Alternative microbial methods: An overview and selection criteria ' (2010 ) 27 Fd Microbiol. : 710 -730.

    • Search Google Scholar
  • Jay, J.M. & Margaritic, S. (1981): Incidence of yeasts in fresh and ground beef and their ratios to bacteria. J. Fd Sci., 46, 648–649.

    Margaritic S. , 'Incidence of yeasts in fresh and ground beef and their ratios to bacteria ' (1981 ) 46 J. Fd Sci. : 648 -649.

    • Search Google Scholar
  • Jay, J.M., Vilai, J.P. & Hughes, M.E. (2003): Profile and activity of the bacterial microbiota of ground beef held from freshness to spoilage at 5–7 °C. Int. J. Fd Microbiol., 81, 105–111.

    Hughes M.E. , 'Profile and activity of the bacterial microbiota of ground beef held from freshness to spoilage at 5–7 °C ' (2003 ) 81 Int. J. Fd Microbiol. : 105 -111.

    • Search Google Scholar
  • Jooste, P.J. & Hugo, C.J. (1999): The taxonomy, ecology and cultivation of bacterial genera belonging to the family Flavobacteriaceae. Int. J. Fd Microbiol., 53, 81–94.

    Hugo C.J. , 'The taxonomy, ecology and cultivation of bacterial genera belonging to the family Flavobacteriaceae. ' (1999 ) 53 Int. J. Fd Microbiol. : 81 -94.

    • Search Google Scholar
  • Justé, A., Thomma, B.P.H.J. & Lievens, B. (2008): Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes. Fd Microbiol., 25, 745–761.

    Lievens B. , 'Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes ' (2008 ) 25 Fd Microbiol. : 745 -761.

    • Search Google Scholar
  • Kulasakara, H., Lee, V., Brencic, A., Liberati, N., Urbach, J., Miyata, S., Lee, D.G., Neely, A.N., Hyodo, M., Hayakawa, Y., Ausubel, F.M. & Lory, S. (2006): Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis (3’-5’)-cyclic GMP in virulence. PNAS, 103, 2839–2844.

    Lory S. , 'Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis (3’-5’)-cyclic GMP in virulence ' (2006 ) 103 PNAS : 2839 -2844.

    • Search Google Scholar
  • Kurtzman, C.P. & Robnett, C.J. (1998): Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek, 73, 331–371.

    Robnett C.J. , 'Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences ' (1998 ) 73 Antonie van Leeuwenhoek : 331 -371.

    • Search Google Scholar
  • Lopandic, K., Zelger, S., Banszky, L.K., Eliskases-Lechner, F. & Prillinger, H. (2006): Identification of yeasts associated with milk products using traditional and molecular techniques. Fd Microbiol., 23, 341–350.

    Prillinger H. , 'Identification of yeasts associated with milk products using traditional and molecular techniques ' (2006 ) 23 Fd Microbiol. : 341 -350.

    • Search Google Scholar
  • Lucianez, A., Holmes, M.A. & Tucker, A.W. (2010): Relationships between processing delay and microbial load of broiler neck skin samples. J. Fd Prot., 73, 26–31.

    Tucker A.W. , 'Relationships between processing delay and microbial load of broiler neck skin samples ' (2010 ) 73 J. Fd Prot. : 26 -31.

    • Search Google Scholar
  • Mahenthiralingam, E., Campbell, M.E., Foster, J., Lam, J.S. & Speert, D.P. (1996): Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J. clin. Microbiol., 34, 1129–1135.

    Speert D.P. , 'Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis ' (1996 ) 34 J. clin. Microbiol. : 1129 -1135.

    • Search Google Scholar
  • Majoros, L., Kardos, G., Belák, Á., Maráz, A., Asztalos, L., Csánky, E., Barta, Z. & Szabó, B. (2003): Restriction enzyme analysis of ribosomal DNA shows that Candida inconspicua clinical isolates can be misidentified as Candida norvegensis with traditional diagnostic procedures. J. clin. Microbiol., 41, 5250–5253.

    Szabó B. , 'Restriction enzyme analysis of ribosomal DNA shows that Candida inconspicua clinical isolates can be misidentified as Candida norvegensis with traditional diagnostic procedures ' (2003 ) 41 J. clin. Microbiol. : 5250 -5253.

    • Search Google Scholar
  • Marchand, S., Heylen, K., Messens, W., Coudijzer, K., de Vos, P., Dewettinck, K. & Herman, L. (2009): Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples. Environ. Microbiol., 11, 467–482.

    Herman L. , 'Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples ' (2009 ) 11 Environ. Microbiol. : 467 -482.

    • Search Google Scholar
  • Mcmeekin, T.A. (1982): Microbial spoilage of meats. -in: Davies, R. (Ed.) Developments in food microbiology. Vol. 1, Applied Science Publ., London, pp. 1–40.

    Mcmeekin T.A. , '', in Developments in food microbiology , (1982 ) -.

  • Mead, G.C. (1982): The microbiology of poultry and game birds. -in: Brown, M.H (Ed.) Meat microbiology. Allied Science Publishers, New York, USA, pp. 67–101.

    Mead G.C. , '', in Meat microbiology. , (1982 ) -.

  • Michiels, C.W., Schellekens, M., Soontjens, C.C.F. & Hauben, K.J.A. (1997): Molecular and metabolic typing of resident and transient fluorescent pseudomonad flora from a meat mincer. J. Fd Prot., 60, 1515–1519.

    Hauben K.J.A. , 'Molecular and metabolic typing of resident and transient fluorescent pseudomonad flora from a meat mincer ' (1997 ) 60 J. Fd Prot. : 1515 -1519.

    • Search Google Scholar
  • Rajmohan, S., Dodd, C.E.R. & Waites, W.M. (2002): Enzymes from isolates of Pseudomonas fluorescens involved in food spoilage. J. appl. Microbiol., 93, 205–213.

    Waites W.M. , 'Enzymes from isolates of Pseudomonas fluorescens involved in food spoilage ' (2002 ) 93 J. appl. Microbiol. : 205 -213.

    • Search Google Scholar
  • Sasahara, K.C. & Zottola, E.A. (1993): Biofilm formation by Listeria monocytogenes utilizes a primary colonizing microorganism in flowing systems. J. Fd Prot., 56, 1022–1028.

    Zottola E.A. , 'Biofilm formation by Listeria monocytogenes utilizes a primary colonizing microorganism in flowing systems ' (1993 ) 56 J. Fd Prot. : 1022 -1028.

    • Search Google Scholar
  • Senses-Ergul, S., Agoston, R., Belák, A. & Deák, T. (2006): Characterization of some yeasts isolated from foods by traditional and molecular tests. Int. J. Fd Microbiol., 108, 120–124.

    Deák T. , 'Characterization of some yeasts isolated from foods by traditional and molecular tests ' (2006 ) 108 Int. J. Fd Microbiol. : 120 -124.

    • Search Google Scholar
  • Sharma, R., Chisti, Y. & Banerjee, U.C. (2001): Production, purification, characterization, and applications of lipases. Biotechnol. Adv., 19, 627–662.

    Banerjee U.C. , 'Production, purification, characterization, and applications of lipases ' (2001 ) 19 Biotechnol. Adv. : 627 -662.

    • Search Google Scholar
  • Stanbridge, L.H. & Davis, A.R. (1998): The microbiology of chill-stored meat. -in: Davis, A. & Board, R. (Eds) Microbiology of meat and poultry. Blackie Publ., London, UK, pp. 175–177.

    Davis A.R. , '', in Microbiology of meat and poultry. , (1998 ) -.

  • Tayeb, L.A., Ageron, E., Grimont, F. & Grimont, P.A.D. (2005): Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates. Res. Microbiol., 156, 763–773.

    Grimont P.A.D. , 'Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates ' (2005 ) 156 Res. Microbiol. : 763 -773.

    • Search Google Scholar
  • Thomas, C.J. & Mcmeekin, T.A. (1984): Effect of water uptake by poultry tissues on contamination by bacteria during immersion in bacterial suspensions. J. Fd Prot., 47, 398–402.

    Mcmeekin T.A. , 'Effect of water uptake by poultry tissues on contamination by bacteria during immersion in bacterial suspensions ' (1984 ) 47 J. Fd Prot. : 398 -402.

    • Search Google Scholar
  • Thomas, C.J., O’Rourke, R.D. & Mcmeekin, T.A. (1987): Bacterial penetration of chicken breast muscle. Fd Microbiol., 4, 87–95.

    Mcmeekin T.A. , 'Bacterial penetration of chicken breast muscle ' (1987 ) 4 Fd Microbiol. : 87 -95.

    • Search Google Scholar
  • Vanderzant, C. & Ousley, T.J. (1963): Utilization of some carbon and nitrogen sources by Pseudomonas fluorescens. J. Fd Sci., 28, 123–129.

    Ousley T.J. , 'Utilization of some carbon and nitrogen sources by Pseudomonas fluorescens. ' (1963 ) 28 J. Fd Sci. : 123 -129.

    • Search Google Scholar
  • Vassart, G., Georges, M., Monsieur, R., Brocas, H., Lequarre, A.S. & Cristophe, D. (1987): A sequence in M13-phage detects hypervariable minisatellites in human and animal DNA. Science, 235, 683–684.

    Cristophe D. , 'A sequence in M13-phage detects hypervariable minisatellites in human and animal DNA ' (1987 ) 235 Science : 683 -684.

    • Search Google Scholar
  • Viljoen, B.C., Gernaras, I., Lamprecht, A. & von Holy, A. (1998): Yeast populations associated with processed poultry. Fd Microbiol., 15, 113–117.

    Holy A. , 'Yeast populations associated with processed poultry ' (1998 ) 15 Fd Microbiol. : 113 -117.

    • Search Google Scholar
  • White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. -in: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds) PCR protocols (A guide to methods and applications). Academic Press Inc., San Diego, pp. 315–322.

    Taylor J. , '', in PCR protocols (A guide to methods and applications). , (1990 ) -.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 4 2
Jul 2020 6 5 2
Aug 2020 6 0 0
Sep 2020 9 0 0
Oct 2020 5 0 0
Nov 2020 8 8 0
Dec 2020 0 0 0