View More View Less
  • 1 Central Food Research Institute Unit of Biology H-1022 Budapest Herman Ottó út 15. Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $878.00

The main goal of our work was to develop a rapid, simple, and economical DNA extraction method for food (especially for meat products) analysis. This extraction and purification procedure was based on the three-phase partitioning (TPP) method. The developed new DNA-TPP method and Wizard DNA Clean-Up System (Promega, USA) have been compared concerning extraction efficiency, purity and DNA suitability for amplification. The quality and quantity of the purified DNA solutions were controlled by spectrophotometer and the amplification efficiency by simple qualitative PCR. All of prepared DNA solutions were pure enough for the PCR and contained appropriate quantity of DNA. Thus, 118 bp length amplicons could have been obtained by the specific lectin-gene PCR in all cases. This method proved to be an alternative one to isolate DNA from meat samples simply and economically.

  • Dennison, C. & Lovrien, R. (1997): Three phase partitioning: Concentration and purification of proteins. Protein Expression and Purification, 11, 149–161.

    Lovrien R. , 'Three phase partitioning: Concentration and purification of proteins ' (1997 ) 11 Protein Expression and Purification : 149 -161.

    • Search Google Scholar
  • Di Pinto, A., Forte, V.T., Guastadisegni, M.C., Martino, C., Schena, F.P. & Tantillo, G. (2007): A comparison of DNA extraction methods for food analysis. Fd Control, 18, 76–80. http://golab.unl.edu/protocols/Extraction.html

    Tantillo G. , 'A comparison of DNA extraction methods for food analysis ' (2007 ) 18 Fd Control : 76 -80.

    • Search Google Scholar
  • Jánosi, A. & Szamos, J. (2001): Comparison of two methods in purification of meat-DNA for PCR. Acta Alimentaria, 30, 113–118.

    Szamos J. , 'Comparison of two methods in purification of meat-DNA for PCR ' (2001 ) 30 Acta Alimentaria : 113 -118.

    • Search Google Scholar
  • Kakihara, Y., Matsufuji, H., Chino, M. & Takeda, M. (2006): Extraction and detection of endogenous soybean DNA from fermented foods. Fd Control, 17, 808–813.

    Takeda M. , 'Extraction and detection of endogenous soybean DNA from fermented foods ' (2006 ) 17 Fd Control : 808 -813.

    • Search Google Scholar
  • Kansal, S., Sharma, A. & Gupta, M.N. (2006): An integrated process for obtaining oil, protease inhibitors and lectin from soybean flour. Fd Res. int., 39, 499–502.

    Gupta M.N. , 'An integrated process for obtaining oil, protease inhibitors and lectin from soybean flour ' (2006 ) 39 Fd Res. int. : 499 -502.

    • Search Google Scholar
  • Meyer, R., Chardonnens, F., Hübner, P. & Lüthy, J. (1996): Polymerase chain reaction (PCR) in the quality and safety assurance of food: detection of soya in processed meat products. Z. Lebensmittel-unters.-Forsch. A, 203, 339–344.

    Lüthy J. , 'Polymerase chain reaction (PCR) in the quality and safety assurance of food: detection of soya in processed meat products ' (1996 ) 203 Z. Lebensmittel-unters.-Forsch. A : 339 -344.

    • Search Google Scholar
  • Odegaard, B.H., Anderson, P.C. & Lovrien, R.E. (1984): Resolution of the multienzyme cellulase complex of Trichoderma reesei QM9414. J. appl. Biochem., 6, 156–183.

    Lovrien R.E. , 'Resolution of the multienzyme cellulase complex of Trichoderma reesei QM9414 ' (1984 ) 6 J. appl. Biochem. : 156 -183.

    • Search Google Scholar
  • Pike, R.N. & Dennison, C. (1989): Protein fractionation by three phase partitioning (TPP) in aqueous/t-butanol mixtures. Biotechnol. Bioengng., 33, 221–228.

    Dennison C. , 'Protein fractionation by three phase partitioning (TPP) in aqueous/t-butanol mixtures ' (1989 ) 33 Biotechnol. Bioengng. : 221 -228.

    • Search Google Scholar
  • Szamos, J. & Hoschke, Á. (1992): Purification of horseradish peroxidase by three-phase partitioning (TPP). Acta Alimentaria, 21, 253–260.

    Hoschke , 'Purification of horseradish peroxidase by three-phase partitioning (TPP) ' (1992 ) 21 Acta Alimentaria : 253 -260.

    • Search Google Scholar
  • Szamos, J. & Kiss, É. (1995): Three-phase partitioning of crude protein extracts. J. Colloid Interface Sci., 170, 290–292.

    Kiss , 'Three-phase partitioning of crude protein extracts ' (1995 ) 170 J. Colloid Interface Sci. : 290 -292.

    • Search Google Scholar
  • Szamos, J., Aubrecht, E. & Gelencsér, É. (1998): Detection of wheat by adapted polymerase chain reaction (PCR) methodology. Acta Alimentaria, 27, 87–95.

    Gelencsér , 'Detection of wheat by adapted polymerase chain reaction (PCR) methodology ' (1998 ) 27 Acta Alimentaria : 87 -95.

    • Search Google Scholar
  • Zimmermann, A., Lüthy, J. & Pauli, U. (1998): Quantitative and qualitative evaluation of nine different extraction methods for nucleic acids on soya bean food samples. Z. Lebensmittel-unters.-Forsch. A, 207, 81–90.

    Pauli U. , 'Quantitative and qualitative evaluation of nine different extraction methods for nucleic acids on soya bean food samples ' (1998 ) 207 Z. Lebensmittel-unters.-Forsch. A : 81 -90.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2020 1 1 0
Dec 2020 1 0 0
Jan 2021 2 0 0
Feb 2021 1 0 0
Mar 2021 1 0 0
Apr 2021 2 0 0
May 2021 0 0 0