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  • 1 Central Food Research Institute Food Safety Department H-1022 Budapest Herman Ottó u. 15 Hungary
  • 2 Corvinus University of Budapest Department of Postharvest, Faculty of Food Science H-1118 Budapest Ménesi út 43-45 Hungary
  • 3 Szent István University Department of Agricultural Biotechnology, Faculty of Agriculture and Environmental Science H-2103 Gödöllő Páter Károly u. 1 Hungary
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In our research we studied the occurrence of the main apple allergen coding gene-families (Mal d 1, Mal d 2, Mal d 3, Mal d 4) in 16 different and most preferably consumed apple varieties. After the DNA isolation by Wizard method the simple PCR reaction was used to examine the apple allergen-coding genes. To identify the presence of the four allergenic protein-coding genes two primer pairs were chosen. The presence of these allergens in most apple varieties could be confirmed. According to our results two varieties — Jonathan and Granny Smith — were found to contain the lowest amount of the coding genes of the allergenic apple proteins studied by us. Besides this, polymorph pattern was obtained by the use of Mal d 1 primer, which may lead to determine apple varieties with small amount of Mal d 1 allergens.The confirmation study of the presence of potential apple allergens by RNA and protein techniques is our plan in the near future.

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